Crystal structure and computational analyses provide insights into the catalytic mechanism of 2,4-diacetylphloroglucinol hydrolase PhlG from Pseudomonas fluorescens

Journal of Biological Chemistry

Volumn 285, Issue 7, 2010, Pages 4603-4611

  He, Yong Xing ^a   Huang, Liang ^a   Xue, Yanyan ^a   Fei, Xue ^a   Teng, Yan Bin ^a   Rubin Pitel, Sheryl B ^b   Zhao, Huimin ^b   Zhou, Cong Zhao ^a  

^a UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA   (China)

^b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYL GROUPS; ACTIVE SITE; AMPHIPHILICS; BOND CLEAVAGES; C-C BONDS; C-TERMINAL DOMAINS; CATALYTIC MECHANISMS; COMPUTATIONAL ANALYSIS; COMPUTATIONAL DOCKINGS; CONFORMATIONAL CHANGE; HYDROLASES; HYDROXYL GROUPS; N-TERMINAL DOMAINS; NUCLEOPHILIC ATTACK; PSEUDOMONAS FLUORESCENS; RARE CLASS; SITE DIRECTED MUTAGENESIS; SUBSTRATE BINDING; SUBSTRATE SPECIFICITY; THREE-DIMENSIONAL STRUCTURE; WATER MOLECULE; ZINC IONS;

BIOCHEMISTRY; CHEMICAL BONDS; COORDINATION REACTIONS; ENZYME ACTIVITY; ENZYMES; STRUCTURAL ANALYSIS; SUBSTRATES; WIND TUNNELS; X RAY CRYSTALLOGRAPHY; ZINC;

CRYSTAL STRUCTURE;

2,4 DIACETYLPHLOROGLUCINOL HYDROLASE; ASPARAGINE; HYDROLASE; HYDROXYL GROUP; PROTEIN PHLG; TYROSINE; UNCLASSIFIED DRUG; WATER; ZINC;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; CRYSTAL STRUCTURE; DIFFRACTION; DIMERIZATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME DEGRADATION; ENZYME MECHANISM; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; HYDROGEN BOND; MATHEMATICAL ANALYSIS; MOLECULAR DOCKING; NONHUMAN; NUCLEOPHILICITY; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN HYDROLYSIS; PSEUDOMONAS FLUORESCENS; SITE DIRECTED MUTAGENESIS; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CATALYSIS; CHROMATOGRAPHY, LIQUID; COMPUTATIONAL BIOLOGY; CRYSTALLOGRAPHY, X-RAY; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PHLOROGLUCINOL; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; PSEUDOMONAS FLUORESCENS; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY; TANDEM MASS SPECTROMETRY;

PSEUDOMONAS FLUORESCENS;

EID: 77951203140     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M109.044180     Document Type: Article

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