The presence of high-molecular-weight viral RNAs interferes with the detection of viral small RNAs

RNA

Volumn 16, Issue 5, 2010, Pages 1062-1067

  Smith, Neil A ^a   Eamens, Andrew L ^a,b   Wang, Ming Bo ^a  

^a CSIRO   (Australia)

^b UNIVERSITY OF SYDNEY   (Australia)

Author keywords

RNA silencing; Satellite RNA; siRNA; Small RNA; Virus

Indexed keywords

GENOMIC RNA; SMALL INTERFERING RNA; VIRUS RNA;

ARTICLE; GEL ELECTROPHORESIS; HYBRIDIZATION; IN VITRO STUDY; MOLECULAR WEIGHT; NORTHERN BLOTTING; PRIORITY JOURNAL; VIRUS DETECTION;

BASE SEQUENCE; CUCUMBER MOSAIC VIRUS SATELLITE; CUCUMOVIRUS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; RNA INTERFERENCE; RNA PROCESSING, POST-TRANSCRIPTIONAL; RNA, SMALL INTERFERING; RNA, VIRAL; TOBACCO;

EID: 77951171711     PISSN: 13558382     EISSN: 14699001     Source Type: Journal    
DOI: 10.1261/rna.2049510     Document Type: Article

References (13)

1. Ahlquist P. 2002. RNA-dependent RNA polymerase, viruses, and RNA silencing. Science 296: 1270-1273.
2. Buck KW. 1996. Comparison of the replication of positive-stranded RNA viruses of plants and animals. Adv Virus Res 47: 159-251.
3. Curtin SJ, Watson JM, Smith NA, Eamens AL, Blanchard CL, Waterhouse PM. 2008. The roles of plant dsRNA-binding proteins in RNAi-like pathways. FEBS Lett 582: 2753-2760.
4. Diaz-Pendon JA, Li F, Li WX, Ding SW. 2007. Suppression of antiviral silencing by cucumber mosaic virus 2b protein in Arabidopsis is associated with drastically reduced accumulation of three classes of viral small interfering RNAs. Plant Cell 19: 2053-2063.
5. Ding SW, Voinnet O. 2007. Antiviral immunity directed by small RNAs. Cell 130: 413-426.
6. Donaire L, Barajas D, Martínez-García B, Martínez-Priego L, Pagán I, Llave C. 2008. Structural and genetic requirements for the biogenesis of Tobacco Rattle Virus-derived small interfering RNAs. J Virol 82: 5167-5177.
7. Ebhardt HA, Unrau PJ. 2009. Characterizing multiple exogenous and endogenous small RNA populations in parallel with subfemtomolar sensitivity using a streptavidin gel-shift assay. RNA 15: 724-731.
8. Gómez G, Martínez G, Pallás V. 2008. Viroid-induced symptoms in Nicotiana benthamiana plants are dependent on RDR6 activity. Plant Physiol 148: 414-423.
9. Lu C, Meyers BC, Green PJ. 2007. Construction of small RNA cDNA libraries for deep sequencing. Methods 43: 110-117.
10. Molnár A, Csorba T, Lakatos L, Várallyay E, Lacomme C, Burgyán J. 2005. Plant virus-derived small interfering RNAs originate predominantly from highly structured single-stranded viral RNAs. J Virol 79: 7812-7818.
11. Qi X, Bao FS, Xie Z. 2009. Small RNA deep sequencing reveals role for Arabidopsis thaliana RNA-dependent RNA polymerases in viral siRNA biogenesis. PLoS One 4: e4971. doi: 10.1371/journal.pone.0004971.
12. Takanami Y. 1981. A striking change in symptoms on cucumber mosaic-virus-infected tobacco plants induced by a satellite RNA. Virol 109: 120-126.
13. Wang MB, Bian XY, Wu LM, Liu LX, Smith NA, Isenegger D, Wu RM, Masuta C, Vance VB, Watson JM, et al. 2004. On the role of RNA silencing in the pathogenicity and evolution of viroids and viral satellites. Proc Natl Acad Sci 101: 3275-3280.