Purification and functional characterization of a Camelid-like single-domain antimycotic antibody by engineering in affinity tag

Protein Expression and Purification

Volumn 72, Issue 1, 2010, Pages 59-65

  Kabir, M Enamul ^a   Krishnaswamy, Senthilkumar ^a   Miyamoto, Masahiko ^a   Furuichi, Yasuhiro ^b   Komiyama, Tadazumi ^a  

^a NIIGATA UNIVERSITY OF PHARMACY AND APPLIED LIFE SCIENCES   (Japan)

^b GeneCare Research Institute   (Japan)

Author keywords

Affinity tag purification; Camelid like antimycotic antibody; HM 1 killer toxin anti idiotypic antibody; PCR based mutagenesis

Indexed keywords

ANTIFUNGAL AGENT; FUNGUS ANTIBODY; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; ANIMAL; ARTICLE; BOVIDS; CANDIDA; CHEMISTRY; DRUG EFFECT; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MUTAGENESIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN TERTIARY STRUCTURE; SACCHAROMYCES; WESTERN BLOTTING;

ANIMALS; ANTIBODIES, FUNGAL; ANTIFUNGAL AGENTS; BLOTTING, WESTERN; CANDIDA; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE EXPRESSION; IMMUNOGLOBULIN VARIABLE REGION; MUTAGENESIS; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; RUMINANTS; SACCHAROMYCES;

ARTIODACTYLA; CAMELIDAE; CANDIDA; ESCHERICHIA COLI; SACCHAROMYCES;

EID: 77951134682     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.01.002     Document Type: Article

References (30)

1. J. Carlsson, J.C. Janson, M. Sparrman, in: J.-C. Christer, L. Ryden (Eds.), Affinity Chromatography, in Protein Purification: Principles, High-resolution, Methods, and Applications, second ed., Wiley-Liss, New York, 1998, pp. 375-442.
2. Waugh D.S. Making the most of affinity tags. Trends Biotechnol. 23 (2005) 316-320
3. Nilsson J., Stahl S., Lunderberg J., Uhlen M., and Nygren P.A. Affinity fusion strategies for detection. Protein Expr. Purif. 11 (1997) 1-16
4. Jarvick J.W., and Telmer C.A. Annu. Rev. Genet. 32 (1998) 601-618
5. DelProposto J., Majmudar C.Y., Smith J.L., and Brown W.C. Mocr: a novel fusion tag for enhancing solubility that is compatible with structural biology applications. Protein Expr. Purif. 63 (2009) 40-49
6. Ford C.F., Suominen I., and Glatz C.E. Fusion tails for the recovery and purification of recombinant proteins. Protein Expr. Purif. 2 (1991) 95-107
7. Porath J., Carlsson J., Olsson I., and Belfrage G. Metal chelate affinity chromatography, a new approach to protein fractionation. Nature 258 (1975) 598-599
8. Porath J. Immobilized metal ion affinity chromatography. Protein Expr. Purif. 4 (1992) 263-281
9. Janknecht R., de Martynoff G., Lou J., Hipskind R.A., Nordheim A., and Stunnenberg H.G. Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus. Proc. Natl. Acad. Sci. USA 88 (1991) 8972-8976
10. Gutierrez R., del Valle E., and Galan M. Immobilized metal-ion affinity chromatography: status and trends. Sep. Purif. Rev. 36 (2007) 71-111
11. Gaberc-Porekar V., and Menart V. Potential for using histidine tags in purification of proteins at large scale. Chem. Eng. Tech. 28 (2005) 1306-1314
12. Changa G. Twenty-five years of immobilized metal ion affinity chromatograph: past present and future. J. Biochem. Biophys. Methods 49 (2001) 313-334
13. Kasahara S., Inoue S.B., Mio T., Yamada T., Nakajima T., Ichishima E., Furuichi Y., and Yamada H. Involvement of cell wall β-glucan in the action of HM-1 killer toxin. FEBS Lett. 348 (1994) 27-32
14. Komiyama T., Ohta T., Urakami H., Shiratori Y., Takasuka T., Sato M., T W., and Y F. Pore formation on proliferating yeast Saccharomyces cerevisiae cell buds by HM-1 killer toxin. J. Biochem. 119 (1996) 731-736
15. Selvakumar D., Miyamoto M., Furuichi Y., and Komiyama T. Inhibition of fungal ß-1, 3-glucan synthase and cell growth by hm-1 killer toxin single-chain anti-idiotypic antibodies. Antimicrob. Agents Chemother. 50 (2006) 3090-3097
16. Krishnaswamy S., Kabir M.E., Miyamoto M., Furuichi Y., and Komiyama T. Cloning antifungal single-chain fragment variable antibodies by phage display and competitive panning elution. Anal. Biochem. 395 (2009) 16-24
17. Selvakumar D., Miyamoto M., Furuichi Y., and Komiyama T. Inhibition of β-1, 3-glucan synthase and cell growth of Cryptococcus species by recombinant single-chain anti-idiotypic antibodies. J. Antibiot. 59 (2006) 73-79
18. Kabir M.E., Krishnaswamy S., Miyamoto M., Furuichi Y., and Komiyama T. An improved phage-display panning method to produce an HM-1 killer toxin anti-idotypic antibody. BMC Biotechnol. 9 (2009) 99
19. Harmsen M.M., and De Haard H.J. Properties, production, and applications of Camelid single-domain antibody fragments. Appl. Microbiol. Biotechnol. 77 (2007) 13-22
20. Conrath K.E., Vincke C., Stijlemans B., Schymkowitz J., Decanniere K., Wyns L., Muyldermans S., and Loris R. Antigen binding and solubility effects upon the veneering of a camel VHH in framework-2 to mimic a VH. J. Mol. Biol. 350 (2005) 112-125
21. L genes, minigenes, and complementarity-determining regions to binding of antibody-combining sites. J. Immunol. 147 (1991) 1709-1719
22. Yau K.Y.F., Tout N.L., Trevors J.T., Lee H., and Hall J.C. Bacterial expression and characterization of a picloram-specific recombinant Fab for residue analysis. J. Agric. Food Chem. 46 (1998) 4457-4463
23. Laemmli U.K. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
24. Komiyama T., Shirai T., Ohta T., Urakami H., Furuichi Y., Ohta Y., and Tsukada Y. Action properties of HYI killer toxin from Williopsis saturnus var. saturnus, and antibiotics, aculeacin A and papulacandin B. Biol. Pharm. Bull. 21 (1998) 1013-1019
25. Schmitt J., Hess H., and Stunnenberg H.G. Affinity purification of histidine-tagged proteins. Mol. Biol. Rep. 18 (1993) 223-230
26. Franken K.L., Hiemstra H.S., Van Meijgaarden K.E., Subronto Y., Den Hartigh J., Ottenhoff T.H., and Drijfhout J.W. Purification of His-tagged proteins by immobilized chelate affinity chromatography: the benefits from the use of organic solvent. Protein Expr. Purif. 18 (2000) 95-99
27. Jiang W., and Hearn M.T. Protein interaction with immobilized metal ion affinity ligands under high ionic strength conditions. Anal. Biochem. 242 (1996) 45-54
28. David N.E., Gee M., Andersen B., Naider F., Thorner J., and Stevens R.C. Expression and purification of the Saccharomyces cerevisiae alpha-factor receptor (Ste2p), a 7-transmembrane-segment G protein-coupled receptor. J. Biol. Chem. 272 (1997) 15553-15561
29. Ko J.K., and Ma J. A rapid and efficient PCR-based mutagenesis method applicable to cell physiology study. Am. J. Physiol. Cell Physiol. 288 (2005) 1273-1278
30. Lu T., Van Dyke M.W., and Sawadogo M. Protein-protein interaction studies using immobilized oligohistidine fusion proteins. Anal. Biochem. 213 (1993) 318-322