Mesenchymal stem or stromal cells: Toward a better understanding of their biology?

Transfusion Medicine and Hemotherapy

Volumn 37, Issue 2, 2010, Pages 75-83

  Lindner, Ulrich ^a   Kramer, Jan ^a   Rohwedel, Jürgen ^a   Schlenke, Peter ^b  

^a UNIVERSITY OF LÜBECK   (Germany)

^b UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

Differentiation; Fibroblasts; MSC; Pericytes; Stem cells

Indexed keywords

CELL DIFFERENTIATION; CELL FUNCTION; CELL ISOLATION; CELL LINEAGE; HUMAN; IMMUNOPHENOTYPING; MESENCHYMAL STEM CELL; MESODERM; NONHUMAN; PHENOTYPE; REVIEW; STROMA CELL;

EID: 77951123227     PISSN: 16603796     EISSN: None     Source Type: Journal    
DOI: 10.1159/000290897     Document Type: Review

References (115)

1. Pittenger MF, Mackay AM, Beck, SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simmonetti DW, Craig S, Marshak DR: Multilineage potential of adult human mesenchymal stem cells. Science 1999;284:143-147.
2. da Silva Meirelles L, Chagastelles PC, Nardi NB: Mesenchymal stem cells reside in virtually all postnatal organs and tissues. J Cell Sci 2006;119:2204-2213.
3. Ferrari G, Cusella-De Angelis G, Coletta M, Paolucci E, Stornaiuolo A, Cossu G, Mavilio F: Muscle regeneration by bone marrow-derived myogenic progenitors. Science 1998;279:1528-1530.
4. Makino S, Fukuda K, Miyoshi S, Konishi F, Kodama H, Pan J, Sano M, Takahashi T, Hori S, Abe H, Hata J, Umezawa A, Ogawa S: Cardiomyocytes can be generated from marrow stromal cells in vitro. J Clin Invest 1999;5:697-705.
5. Hakuno D, Fukuda K, Makino S, Konishi F, Tomita Y, Manabe T, Suzuki Y, Umezawa A, Ogawa S: BM-derived regenerated cardiomyocytes (CMG cells) express functional adrenergic and muscarinic receptors. Circulation 2002;105:380-386.
6. Franco Lambert AP, Fraga Zandonai A, Bonatto D, Cantarelli Machado D, Pêgas Henriques JA: Differentiation of human adipose-derived adult stem cells into neuronal tissue: does it work? Differentiation 2009;77:221-228.
7. Schäfer R, Northoff H: Cardioprotection and cardiac regeneration by mesenchymal stem cells. Panminerva Med 2008;50:31-39.
8. Wagers AJ, Weissman IL: Plasticity of adult stem cells. Cell 2004;116:639-648.
9. Mackay AM, Beck SC, Murphy JM, Barry FP, Chichester CO, Pittenger MF: Chondrogenic differentiation of cultured human mesenchymal stem cells from marrow. Tissue Eng 1998;4:415-428.
10. Lee KL, Kuo TK, Chen WM, Lee KD, Hsieh SL, Chen TH: Isolation of multi-potent mesenchymal stem cells from umbilical cord blood. Blood 2003; 103:1669-1675.
11. Kern S, Eichler H, Stoeve J, Klüter H, Bieback K: Comparative analysis of mesenchymal stem cells from bm, umbilical cord blood, or adipose tissue. Stem Cells 2006;24:1294-1301.
12. Rotter N, Oder J, Schlenke P, Lindner U, Böhrnsen F, Kramer J, Rohwedel J, Huss R, Brandau S, Wollenberg B, Lang S: Isolation and characterization of adult stem cells from human salivary glands. Stem Cells Dev 2008;17:509-518.
13. Lanzoni G, Alviano F, Marchionni C, Bonsi L, Costa R, Foroni L, Roda G, Belluzzi A, Caponi A, Ricci F, Luigi Tazzari P, Pagliaro P, Rizzo R, Lanza F, Roberto Baricordi O, Pasquinelli G, Roda E, Paolo Bagnara G: Isolation of stem cell populations with trophic and immunoregulatory functions from human intestinal tissues: potential for cell therapy in inflammatory bowel disease. Cytotherapy 2009;8:1020-1031.
14. Moore KA, Lemischka IR: Stem cells and their niches. Science 2006;311:1880-1885.
15. Bradley A, Evans M, Kaufman MH, Robertson E: Formation of germ-line chimaeras from embryoderived teratocarcinoma cell lines. Nature 1984; 309:255-256.
16. Baksh D, Song L, Tuan RS: Adult mesenchymal stem cells: characterization, differentiation, and application in cell and gene therapy. J Cell Mol Med 2004;8:301-316.
17. Prockop DJ: Marrow stromal cells as stem cells for nonhematopoietic issues. Science 1997;276:71-74.
18. Schäfer R, Dominici M, Müller I, Dazzi F, Bieback K, Godthardt K, Le Blanc K, Meisel R, Pochampally R, Richter R, Skutella T, Steinhoff G, Mitterberger M, Wendel H, Wiskirchen J, Handgretinger R, Northoff H: Progress in characterization, preparation and clinical applications of nonhematopoietic stem cells, 29-30 September 2006, Tübingen, Germany. Cytotherapy 2007;9:397-405.
19. Ratajczak MZ, Zuba-Surma EK, Wysoczynski M, Wan W, Ratajczak J, Wojakowski W, Kucia M: Hunt for pluripotent stem cell - regenerative medicine search for almighty cell. J Autoimmun 2008; 30:151-162
20. Jiang Y, Jahagirdar BN, Reinhardt RL, Schwartz RE, Keene CD, Ortiz-Gonzalez XR, Reyes M, Lenvik T, Lund T, Blackstad M, Du J, Aldrich S, Lisberg A, Low WC, Largaespada DA, Verfaillie CM: Pluripotency of mesenchymal stem cells derived from adult marrow. Nature 2002;418:41-49.
21. D'Ippolito G, Diabira S, Howard GA, Menei P, Roos BA, Schiller PC: Marrow-isolated adult multilineage inducible (MIAMI) cells, a unique population of postnatal young and old human cells with extensive expansion and differentiation potential. J Cell Sci 2004;117:2971-2981.
22. Beltrami AP, Cesselli D, Bergamin N, Marcon P, Rigo S, Puppato E, D'Aurizio F, Verardo R, Piazza S, Pignatelli A, Poz A, Baccarani U, Damiani D, Fanin R, Mariuzzi L, Finato N, Masolini P, Burelli S, Belluzzi O, Schneider C, Beltrami CA: Multipotent cells can be generated in vitro from several adult human organs (heart, liver, and bone marrow). Blood 2007;110:3438-3446.
23. Kolf CM, Cho E, Tuan RS: Mesenchymal stromal cells. biology of adult stem cells: regulation of niche, self-renewal and differentiation. Arthritis Res Ther 2007;7:204.
24. Meirelles LdS, Caplan AI, Nardi NB: In search of the in vivo identity of mesenchymal stem cells. Stem Cells 2008;26:2287-2299.
25. Quinn C, Flake AW: In vivo differentiation potential of mesenchymal stem cells: prenatal and postnatal model systems. Transfus Med Hemother 2008; 35:239-247.
26. Kramer J, Böhrnsen F, Lindner U, Behrens P, Schlenke P, Rohwedel J: In vivo matrix-guided human mesenchymal stem cells. Cell Mol Life Sci 2006;63:616-626.
27. Friedenstein AJ, Chailakhjan RK, Lalykina KS: The development of fibroblast colonies in monolayer cultures of guinea-pig BM and spleen cells. Cell Tissue Kinet 1970;3:393-403.
28. Friedenstein AJ, Goskaja UF, Julagina NN: Fibroblast precursors in normal and irradiated mouse hematopoietic organs. Exp Hematol 1976;4:267-274.
29. Sacchetti B, Funari A, Michienzi S, Di Cesare S, Piersanti S, Saggio I, Tagliafico E, Ferrari S, Robey PG, Riminucci M, Bianco P: Self-renewing osteoprogenitors in bone marrow sinusoids can organize a hematopoietic microenvironment. Cell 2007;131:324-336.
30. Owen M: Lineage of osteogenic cells and their relationship to the stromal system; in Peck WA (ed): Bone and Mineral Research, vol.3. New York Elsevier, 1985, pp 1-25.
31. Gronthos S, Simmons PJ: The Growth Factor Requirements of STRO-1-positive human BM stromal percusors under serum-deprived conditions in vitro. Blood 1995;85:929-940.
32. Kutnetsov SA, Friedenstein AJ, Robey PG: Factors required for bone-marrow stromal fibroblast colony formation in vitro. Br J Hematol 1997;97: 561-570.
33. Baddoo D, Hill K, Wilkinson R, Gaupp D, Hughes C, Kopen GC, Phinney DG: Characterization of mesenchymal stem cells isolated from bone-marrow by negative selection. J Cell Biochem 2003;89: 1235-1249.
34. Bianchi G, Banfi A, Mastrogiacomo M, Notaro R, Luzzatto L, Cancedda R, Quarto R: Ex vivo enrichment of mesenchymal stem cell progenitors by fibroblast growth factor 2. Exp Cell Res 2003;287: 98-105.
35. Wang QR, Yan ZJ, Wolf NS: Dissecting the hematopoietic microenvironment VI. The effects of several growth factors on the growth of murine bone marrow CFU-F. Exp Hematol 1990;18:341-347.
36. Caplan AI: Mesenchymal stem cells. J Orthop Res 1991;9:641-650.
37. Caplan AI: The Mesengenic Process. Clin Plast Surg 1994;21:429-435.
38. Caplan AI: Osteogensis imperfecta, rehabilitation medicine, fundamental research and mesenchymal stem cells. Connect Tissue Res 1995;31:S9-14.
39. Dominici M, LeBlanc K, Mueller I, Slaper-Cortenbach I, Marina F, Krause D, Deans R, Keating A, Prockop D, Horwitz E: Minimal criteria for defining mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 2006;8:315-317.
40. Haniffa MA, Collin MP, Buckley CD, Dazzi F: Mesenchymal stem cells: the fibroblasts' new clothes? Haematologica 2009;94:258-263.
41. Wagner W, Wein F, Seckinger A, Frankhauser M, Wirkner U, Krause U, Blake J, Schwager C, Eckstein V, Ansorge W, Ho AD: Comparative characteristics of mesenchymal stem cells from human bone marrow, adipose tissue, and umbilical cord blood. Exp Hematol 2005;33:1402-1416.
42. Wagner W, Ho AD: Mesenchymal stem cell preparations - comparing apples and oranges. Stem Cell Rev 2007;3:239-248.
43. Haniffa MA, Wang XN, Holtick U, Rae M, Isaacs JD, Dickinson AM, Hilkens CM, Collin MP: Adult human fibroblasts are potent immunoregulatory cells and functionally equivalent to mesenchymal stem cells. J Immunol 2007;179:1595-1604.
44. Huang HI, Chen SK, Ling QD, Chien CC, Liu HT, Chan SH: Multilineage differentiation potential of fibroblast-like stromal cells derived from human skin. Tissue Eng Part A; doi:10.1089/ten. tea.2009.0431.
45. Friedenstein AJ, Lalykina KS: Thymus cells are inducible to osteogenesis. Eur J Immunol 1972;6: 602-606.
46. Roufosse CA, Direkze NC, Otto WR, Wright NA: Circulating mesenchymal stem cells. Int J Biochem Cell Biol 2004;36:585-597.
47. Katz AJ, Tholpady A, Tholpady SS, Shang H, Ogle RC: Cell surface and transcriptional characterization of human adipose-derived adherent stromal (hADAS) cells. Stem Cells 2005;3:412-423.
48. Mitchell JB, McIntosh K, Zvonic S, Garrett S, Floyd ZE, Kloster A, Di Halvorsen Y, Storms RW, Goh B, Kilroy G, Wu X, Gimble JM: Immunophenotype of human mesenchymal stem cells cells: temporal changes in stromal-associated and stem cell-associated markers. Stem Cells 2006;24:376-385.
49. Kilroy GE, Foster SJ, Wu X, Ruiz J, Sherwood S, Heifetz A, Ludlow JW, Stricker DM, Potiny S, Green P, Halvorsen YD, Cheatham B, Storms RW, Gimble JM: Cytokine profile of adult stem cells: expression of angiogenic, hematopoietic, and pro-inflammatory factors. J Cell Physiol 2007;212:702-709.
50. Rodriguez AM, Elabd C, Delteil F, Astier J, Vernochet C, Saint-Marc P, Guesnet J, Guezennec A, Amri EZ, Dani C, Ailhaud G: Adipocyte differentiation of multipotent cells established from human adipose tissue. Biochem Biophys Res Commun 2004;315:255-263.
51. Gimble JM, Dorheim MA, Cheng Q, Medina K, Wang CS, Jones R, Koren E, Pietrangeli C, Kincade PW: Adipogenesis in a murine BM stromal cell line capable of supporting B lineage lymphocyte growth and proliferation: bio-chemical and molecular characterization. Eur J Immunol 1990; 20:379-387.
52. Halvorsen YD, Bond A, Sen A, Franklin DM, Lea-Currie YR, Sujkowski D, Ellis PN, Wilkison WO, Gimble JM: Thiazolidinediones and glucocorticoids synergistically induce differentiation of human adipose tissue stromal cells: biochemical, cellular, and molecular analysis. Metabolism 2001; 50:407-413.
53. Sen A, Lea-Currie YR, Sujkowska D, Franklin DM, Wilkison WO, Halvorsen YD, Gimble JM: Adipogenic potential of human adipose derived stromal cells from multiple donors is heterogeneous. J Cell Biochem 2001;81:312-319.
54. Jaiswal N, Haynesworth SE, Caplan AI, Bruder SP: Osteogenic differentiation of purified, cultureexpanded human mesenchymal stem cells in vitro. J Cell Biochem 1997;64:295-312.
55. Heino TJ, Hentunen TA: Differentiation of osteoblasts and osteocytes from mesenchymal stem cells. Curr Stem Cell Res Ther 2008;2:131-145.
56. Chevallier N, Anagnostou F, Zilber S, Bodivit G, Maurin S, Barrault A, Bierling P, Hernigou P, Layrolle P, Rouard H: Osteoblastic differentiation of human mesenchymal stem cells with platelet lysate. Biomaterials 2010;31:270-278.
57. Gronthos S, Graves SE, Ohta S, Simmons PJ: The STRO-1+ fraction of adult human BM contains the osteogenic precursors. Blood 1994;84:4164-4173.
58. Gronthos S, Zannettino AC, Graves SE, Ohta S, Hay SJ, Simmons PJ: Differential cell surface expression of the STRO-1 and alkaline phosphatase antigens on discrete developmental stages in primary cultures of human bone cells. J Bone Miner Res 1999;14:47-56.
59. Pelttari K, Steck E, Richter W: The use of mesenchymal stem cells for chondrogenesis. Injury 2008; 39(suppl 1):S58-65.
60. Erickson IE, Huang AH, Chung C, Li RT, Burdick JA, Mauck RL: Differential maturation and structure- function relationships in mesenchymal stem cell- and chondrocyte-seeded hydrogels. Tissue Eng Part A 2009;15:1041-1052.
61. Böhrnsen F, Lindner U, Meier M, Gadallah A, Schlenke P, Lehnert H, Rohwedel J, Kramer J: Murine mesenchymal progenitor cells from different tissues differentiated via mesenchymal microspheres into the mesodermal direction. BMC Cell Biol 2009;10:92.
62. Butler DL, Goldstein SA, Guilak F: Functional tissue engineering: the role of biomechanics. J Biomech Eng 2000;122:570-575.
63. Shao XX, Hutmacher DW, Ho ST, Goh JC, Lee EH: Evaluation of a hybrid scaffold/cell construct in repair of high-load-bearing osteochondral defects in rabbits. Biomaterials 2006;27:1071-1080.
64. Betre H, Ong SR, Guilak F, Chilkoti A, Fermor B, Setton LA: Chondrocytic differentiation of human adipose-derived adult stem cells in elastin-like polypeptide. Biomaterials 2006;27:91-99. (Pubitemid 41225372)
65. Guilak F, Cohen DM, Estes BT, Gimble JM, Liedtke W, Chen CS: Control of stem cell fate by physical interactions with the extracellular matrix. Cell Stem Cell 2009;1:17-26.
66. Wang DW, Fermor B, Gimble JM, Awad HA, Guilak F: Influence of oxygen on the proliferation and metabolism of adipose derived adult stem cells. J Cell Physiol 2005;204:184-191.
67. Muraglia A, Cancedda R, Quarto R: Clonal mesenchymal progenitors from human bone marrow differentiate in vitro according to a hierarchical model. J Cell Sci 2000;113:1161-1166.
68. Bilkovski R, Schulte DM, Oberhauser F, Gomolka M, Udelhoven M, Hettich MM, Roth B, Heidenreich A, Gutschow C, Krone W, Laudes M: Role of wnt-5a in the determination of human mesenchymal stem cells into preadipocytes. J Biol Chem 2010;285:6170-6178.
69. Kim WK, Meliton V, Amantea CM, Hahn TJ, Parhami F: 20(S)- hydroxycholesterol inhibits PPARgamma expression and adipogenic differentiation of bone-marrow stromal cells through a hedgehogdependent mechanism. J Bone Miner Res 2007; 22:1711-1719.
70. Lecka-Czernik B, Moerman EJ, Grant DF, Lehmann JM, Manolagas SC, Jilka RL: Divergent effects of selective peroxisome proliferator-activated receptor-gamma 2 ligands on adipocyte versus osteoblast differentiation. Endocrinology 2002;143: 2376-2384.
71. Duque G, Rivas D: Alendronate has an anabolic effect on bone through the differentiation of mesenchymal stem cells. J Bone Miner Res 2007;10: 1603-1611.
72. Bowers RR, Lane MD: A role for bone morphogenetic protein-4 in adipocyte development. Cell Cycle 2007;6:385-389.
73. Rojewski MT, Weber BM, Schrezenmeier H: Phenotyping characterization of mesenchymal stem cells from various tissues. Transfus Med Hemother 2008;35:168-184.
74. Pontikoglou C, Delorme B, Charbord P: Human bone-marrow native mesenchymal stem cells. Regen Med 2008;5:731-741.
75. Bühring HJ, Treml S, Cerabona F, de Zwart P, Kanz L, Sobiesiak M: Phenotypic characterization of distinct human bone-marrow-derived MSC Subsets. Annu N Y Acad Sci 2009;1176:124-134.
76. Jones EA, English A, Kinsey SE, Straszynski L, Emery P, Ponchel F, McGonagle D: Optimization of a flow cytometry-based protocol for detection and phenotypic characterization of multipotent mesenchymal stromal cells from human BM. Cytometry B Clin Cytom 2006;70:391-399.
77. Quirici N, Soligo D, Bossolasco P, Servida F, Lumini C, Deliliers GL: Isolation of bone marrow mesenchymal stem cells by anti-nerve growth factor receptor antibodies. Exp Hematolol 2002;7:783-791.
78. Morrison SJ, Uchida N, Weissman IL: The biology of hematopoietic stem cells. Annu Rev Cell Dev Biol 1995;11:35-71.
79. Clark BR, Jamieson C, Keating A: Human longterm bone-marrow culture. Methods Mol Biol 1997; 75:249-256.
80. Bianco P, Robey PG: Stem cells in tissue engineering. Nature 2001;414:118-121.
81. Bianco P, Riminucci M, Gronthos S, Robey PG: Bone-marrow stromal stem cells: nature, biology, and potential applications. Stem Cells 2001;19:180-192.
82. Bianco P, Robey PG, Simmons PJ: Mesenchymal stem cells: revisiting history, concepts, and assay. Cell Stem Cell 2009;2,313-319.
83. Horwitz EM, Le Blanc K, Dominici M, Mueller I, Slaper-Cortenbach I, Marini FC, Deans RJ, Krause DS, Keating A; International Society for Cellular Therapy: Clarification of the nomenclature for MSC: the International Society for Cellular Therapy position statement. Cytotherapy 2005;7:393-395.
84. Dellavalle A, Sampaolesi M, Tonlorenzi R, Tagliafico E, Sacchetti B, Perani L, Innocenzi A, Galvez BG, Messina G, Morosetti R, Li S, Belicchi M, Peretti G, Chamberlain JS, Wright WE, Torrente Y, Ferrari S, Bianco P, Cossu G: Pericytes of human skeletal muscle are myogenic precursors distinct from satellite cells. Nature Cell Biol 2007; 9:255-267.
85. Gronthos S, Franklin DM, Leddy HA, Robey PG Storms RW, Gimble JM: Surface protein characterization of human adipose tissue-derived stromal cells. J Cell Physiol 2001;189:54-63.
86. Rodriguez AM, Elabd C, Amri EZ, Ailhaud G, Dani C: The human adipose tissue is a source of multipotent stem cells. Biochemie 2005;1:125-128.
87. Lindroos B, Boucher S, Chase L, Kuokkanen H, Huhtala H, Haataja R, Vemuri M, Suuronen R, Miettinen S: Serum-free, xeno-free culture media maintain the proliferation rate and multipotentiality of adipose stem cells in vitro. Cytotherapy 2009; 11:958-972.
88. Schreml S, Babilas P, Fruth S, Orsó E, Schmitz G, Mueller MB, Nerlich M, Prantl L: Harvesting human adipose tissue-derived adult stem cells: resection versus liposuction. Cytotherapy 2009;11: 947-957.
89. Sensebé L, Bourin P: Mesenchymal stem cells for therapeutic purposes. Transplantation 2009;15 (9 suppl):S49-53.
90. Alsalameh S, Amin R, Gemba T, Lotz M: Identification of mesenchymal progenitor cells in normal and osteoarthritic human articular cartilage. Arthritis Rheum 2004;5:1522-1532.
91. Hiraoka K, Grogan S, Olee T, Lotz M: Mesenchymal progenitor cells in adult human articular cartilage. Biorheology 2006;43:447-454.
92. Secco M, Moreira YB, Zucconi E, Vieira NM, Jazedje T, Muotri AR, Okamoto OK, Verjovski- Almeida S, Zatz M: Gene expression profile of mesenchymal stem cells from paired umbilical cord units: cord is different from blood. Stem Cell Rev 2009;5,387-401.
93. Jäger M, Zilkens C, Bittersohl B, Krauspe R: Cord blood - an alternative source for bone regeneration. Stem Cell Rev 2009;5: 266-277.
94. Bieback K, Klüter H: Mesenchymal stromal cells from umbilical cord blood. Curr Stem Cell Res Ther 2007;2:310-323.
95. Campagnoli C, Roberts IA, Kumar S, Bennett PR, Bellantuono I, Fisk NM: Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood, liver, and bone marrow. Blood 2001;8,2396-2402.
96. Nakamura S, Yamada Y, Katagiri W, Sugito T, Ito K, Ueda M: Stem cell proliferation pathways comparison between human exfoliated deciduous teeth and dental pulp stem cells by gene expression profile from promising dental pulp. J Endod 2009; 11:1536-1542.
97. Huang GT, Gronthos S, Shi S: Mesenchymal stem cells derived from dental tissues vs. those from other sources: their biology and role in regenerative medicine. J Dent Res 2009;9:792-806.
98. Valtieri M, Sorrentino A: The mesenchymal stromal cell contribution to homeostasis. J Cell Physiol 2008;296:296-300.
99. Shi S, Bartold PM, Miura M, Seo BM, Robey PG, Gronthos S: The efficacy of mesenchymal stem cells to regenerate and repair dental structures. Orthod Craniofac Res 2005;3:191-199.
100. O'Driscoll SW, Fitzsimmons JS: The role of periosteum in cartilage repair. Clinical Orthop Relat Res 2001;(391 suppl):S190-207.
101. de Mos M, Koevoet WJ, Jahr H, Verstegen MM, Heijboer MP, Kops N, van Leeuwen JP, Weinans H, Verhaar JA, van Osch GJ: Intrinsic differentiation potential of adolescent human tendon tissue: an in-vitro cell differentiation study. BMC Musculoskelet Disord 2007;8:16.
102. Meirelles Lda S, Nardi NB: Methodology, biology and clinical applications of mesenchymal stem cells. Front Biosci 2009;14:4281-4298.
103. Rodríguez JP, Astudillo P, Ríos S, Pino AM: Involvement of adipogenic potential of human BM mesenchymal stem cells (MSCs) in osteoporosis. Curr Stem Cell Res Ther 2008;3:208-218.
104. Rickard DJ, Wang FL, Rodriguez-Rojas AM, Wu Z, Trice WJ, Hoffman SJ, Votta B, Stroup GB, Kumar S, Nuttall ME: Intermittent Treatment with parathyroid hormone (PTH) as well as a non-peptide small molecule agonist of the PTH1 receptor inhibits adipocyte differentiation in human bone marrow stromal cells. Bone 2006;39:1361-1372.
105. Chen WH, Lai MT, Wu AT, Wu CC, Gelovani JG, Lin CT, Hung SC, Chiu WT, Deng WP: In vitro stage-specific chondrogenesis of mesenchymal stem cells committed to chondrocytes. Arthritis Rheum 2009;60:450-459.
106. Djouad F, Mrugala D, Noël D, Jorgensen C: Engineered mesenchymal stem cells for cartilage repair. Regen Med 2006;1:529-537.
107. Santiago JA, Pogemiller R, Ogle BM: Heterogeneous differentiation of human mesenchymal stem cells in response to extended culture in extracellular matrices. Tissue Eng Part A 2009;15: 3911-3922.
108. Song IH, Caplan AI, Dennis JE: In vitro dexamethasone pretreatment enhances bone formation of human mesenchymal stem cells in vivo. J Orthop Res 2009;27:916-921.
109. Kassem M, Abdallah BM, Saeed H: Osteoblastic cells: differentiation and trans-differentiation. Arch Biochem Biophys 2008;473:183-187.
110. Meirelles Lda S, Nardi MB: Murine marrow derived mesenchymal stem cell: isolation, in vitro expansion, and characterization. Br J Hematol 2003; 123:702-711.
111. Vogel W, Grünebach F, Messam CA, Kanz L, Brugger W, Bühring HJ: Heterogeneity among human BM-derived mesenchymal stem cells and neural progenitor cells. Haematologica 2003;88: 126-133.
112. Verfaillie CM: Anatomy and physiology of hematopoiesis; in Hoffman R, Strauss M, Benz EJJ, et al (eds): Hematology - Basic Principles and Practice, 3rd ed. Philadelphia, Churchill-Livingstone, 2000, pp 139-154.
113. Buhring HJ, Battula VL, Trml S, Schewe, B, Kanz L, Vogel W: Novel markers for the prospective isolation of human MSC. Annu N Y Acad Sci 2007; 1106:262-271.
114. Schäfer R: Labeling and imaging of stem cells - promises and concerns. Transfus Med Hemother 2010;37(2):85-89.
115. Freimark D, Pino-Grace P, Pohl S, Weber C, Wallrapp C, Geigle P, Pörtner R, Czermak P: Use of encapsulated stem cells to overcome the bottleneck of cell availability for cell therapy approaches. Transfus Med Hemother 2010;37(2): 66-73.