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Volumn 11, Issue , 2010, Pages

Elasmobranch qPCR reference genes: A case study of hypoxia preconditioned epaulette sharks

Author keywords

[No Author keywords available]

Indexed keywords

BETA TUBULIN; DNA DIRECTED RNA POLYMERASE II POLYPEPTIDE F; EUKARYOTIC TRANSLATION ELONGATION FACTOR 1 BETA; MEMBRANE PROTEIN; RIBOSOME PROTEIN; RIBOSOME PROTEIN L6; UBIQUITIN; UNCLASSIFIED DRUG; ELONGATION FACTOR 1; MESSENGER RNA; RNA POLYMERASE II;

EID: 77951109455     PISSN: None     EISSN: 14712199     Source Type: Journal    
DOI: 10.1186/1471-2199-11-27     Document Type: Article
Times cited : (16)

References (42)
  • 1
    • 34548856245 scopus 로고    scopus 로고
    • Insights from sharks: Evolutionary and developmental models of fin development
    • Cole NJ, Currie PD. Insights from sharks: Evolutionary and developmental models of fin development. Dev Dynamics 2007, 236:2421-2431.
    • (2007) Dev Dynamics , vol.236 , pp. 2421-2431
    • Cole, N.J.1    Currie, P.D.2
  • 2
    • 0033740604 scopus 로고    scopus 로고
    • The effects of fishing on sharks, rays, and chimaeras (chondrichthyans), and the implications for marine ecosystems
    • Stevens JD, Bonfil R, Dulvy NK, Walker PA. The effects of fishing on sharks, rays, and chimaeras (chondrichthyans), and the implications for marine ecosystems. ICES J Mar Sci 2000, 57:476-494.
    • (2000) ICES J Mar Sci , vol.57 , pp. 476-494
    • Stevens, J.D.1    Bonfil, R.2    Dulvy, N.K.3    Walker, P.A.4
  • 3
    • 0029667387 scopus 로고    scopus 로고
    • Scales of thelodont and shark-like fishes from the Ordovician of Colorado
    • Sansom IJ, Smith MM, Smith MP. Scales of thelodont and shark-like fishes from the Ordovician of Colorado. Nature 1996, 379:628-630.
    • (1996) Nature , vol.379 , pp. 628-630
    • Sansom, I.J.1    Smith, M.M.2    Smith, M.P.3
  • 4
    • 34247342594 scopus 로고    scopus 로고
    • Survey sequencing and comparative analysis of the elephant shark (Callorhinchus milii) genome
    • Venkatesh B, Kirkness EF, Loh YH, Halpern AL, Lee AP, Johnson J, et al. Survey sequencing and comparative analysis of the elephant shark (Callorhinchus milii) genome. PLoS Biology 2007, 5:932-944.
    • (2007) PLoS Biology , vol.5 , pp. 932-944
    • Venkatesh, B.1    Kirkness, E.F.2    Loh, Y.H.3    Halpern, A.L.4    Lee, A.P.5    Johnson, J.6
  • 5
    • 0000739999 scopus 로고    scopus 로고
    • Chondrichthyes
    • London: Chapman and Hall, Benton MJ
    • Cappetta H, Duffin C, Zidek J. Chondrichthyes. The fossil record 2 2003, 593-609. London: Chapman and Hall, Benton MJ.
    • (2003) The fossil record 2 , pp. 593-609
    • Cappetta, H.1    Duffin, C.2    Zidek, J.3
  • 6
    • 0033775615 scopus 로고    scopus 로고
    • Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
    • 10.1677/jme.0.0250169, 11013345
    • Bustin SA. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 2000, 25:169-193. 10.1677/jme.0.0250169, 11013345.
    • (2000) J Mol Endocrinol , vol.25 , pp. 169-193
    • Bustin, S.A.1
  • 7
    • 41449100793 scopus 로고    scopus 로고
    • Improved normalization of real-time reverse transcriptase polymerase chain reaction data using an external RNA control
    • 10.1016/j.ab.2008.01.028, 18294949
    • Ellefsen S, Stenslokken KO, Sandvik GK, Kristensen TA, Nilsson GE. Improved normalization of real-time reverse transcriptase polymerase chain reaction data using an external RNA control. Anal Biochem 2008, 376:83-93. 10.1016/j.ab.2008.01.028, 18294949.
    • (2008) Anal Biochem , vol.376 , pp. 83-93
    • Ellefsen, S.1    Stenslokken, K.O.2    Sandvik, G.K.3    Kristensen, T.A.4    Nilsson, G.E.5
  • 8
    • 45249097803 scopus 로고    scopus 로고
    • Acute and chronic hypoxia affects HIF-1 alpha mRNA levels in sea bass (Dicentrarchus labrax)
    • Terova G, Rimoldi S, Cora S, Bernardini G, Gornati R, Saroglia M. Acute and chronic hypoxia affects HIF-1 alpha mRNA levels in sea bass (Dicentrarchus labrax). Aquaculture 2008, 279:150-159.
    • (2008) Aquaculture , vol.279 , pp. 150-159
    • Terova, G.1    Rimoldi, S.2    Cora, S.3    Bernardini, G.4    Gornati, R.5    Saroglia, M.6
  • 9
    • 21344458602 scopus 로고    scopus 로고
    • Quantitative real-time RT-PCR - a perspective
    • 10.1677/jme.1.01755, 15956331
    • Bustin SA, Benes V, Nolan T, Pfaffl MW. Quantitative real-time RT-PCR - a perspective. J Mol Endocrinol 2005, 34:597-601. 10.1677/jme.1.01755, 15956331.
    • (2005) J Mol Endocrinol , vol.34 , pp. 597-601
    • Bustin, S.A.1    Benes, V.2    Nolan, T.3    Pfaffl, M.W.4
  • 10
    • 33845807514 scopus 로고    scopus 로고
    • Toxicological housekeeping genes: Do they really keep the house?
    • 10.1021/es0615223, 17256553
    • Arukwe A. Toxicological housekeeping genes: Do they really keep the house?. Environ Sci Technol 2006, 40:7944-7949. 10.1021/es0615223, 17256553.
    • (2006) Environ Sci Technol , vol.40 , pp. 7944-7949
    • Arukwe, A.1
  • 11
    • 0036024287 scopus 로고    scopus 로고
    • Oxygen-dependent cellular functions - why fishes and their aquatic environment are a prime choice of study
    • Nikinmaa M. Oxygen-dependent cellular functions - why fishes and their aquatic environment are a prime choice of study. Comp Biochem Physiol A 2002, 133:1-16.
    • (2002) Comp Biochem Physiol A , vol.133 , pp. 1-16
    • Nikinmaa, M.1
  • 12
    • 0029400202 scopus 로고
    • Oxygen transfer in fish: Morphological and molecular adjustments
    • Val AL. Oxygen transfer in fish: Morphological and molecular adjustments. Braz J Med Biol Res 1995, 28:1119-1127.
    • (1995) Braz J Med Biol Res , vol.28 , pp. 1119-1127
    • Val, A.L.1
  • 13
    • 4644324293 scopus 로고    scopus 로고
    • Hypoxic survival strategies in two fishes: extreme anoxia tolerance in the North European crucian carp and natural hypoxic preconditioning in a coral-reef shark
    • 10.1242/jeb.00979, 15299034
    • Nilsson GE, Renshaw GMC. Hypoxic survival strategies in two fishes: extreme anoxia tolerance in the North European crucian carp and natural hypoxic preconditioning in a coral-reef shark. J Exp Biol 2004, 207:3131-3139. 10.1242/jeb.00979, 15299034.
    • (2004) J Exp Biol , vol.207 , pp. 3131-3139
    • Nilsson, G.E.1    Renshaw, G.M.C.2
  • 15
    • 39849089161 scopus 로고    scopus 로고
    • Housekeeping genes for quantitative expression studies in the three-spined stickleback Gasterosteus aculeatus
    • 10.1186/1471-2199-9-18, 2254436, 18230138
    • Hibbeler S, Scharsack JP, Becker S. Housekeeping genes for quantitative expression studies in the three-spined stickleback Gasterosteus aculeatus. BMC Mol Biol 2008, 9:18. 10.1186/1471-2199-9-18, 2254436, 18230138.
    • (2008) BMC Mol Biol , vol.9 , pp. 18
    • Hibbeler, S.1    Scharsack, J.P.2    Becker, S.3
  • 16
    • 56949096503 scopus 로고    scopus 로고
    • Selection of reference genes for qRT-PCR examination of wild populations of Atlantic cod Gadus morhua
    • 10.1186/1756-0500-1-47, 2527504, 18710500
    • Olsvik PA, Søfteland L, Lie KK. Selection of reference genes for qRT-PCR examination of wild populations of Atlantic cod Gadus morhua. BMC Res Notes 2008, 1:47. 10.1186/1756-0500-1-47, 2527504, 18710500.
    • (2008) BMC Res Notes , vol.1 , pp. 47
    • Olsvik, P.A.1    Søfteland, L.2    Lie, K.K.3
  • 17
    • 52149099642 scopus 로고    scopus 로고
    • Stability of reference genes for real-time PCR analyses in channel catfish (Ictalurus punctatus) tissues under varying physiological conditions
    • 10.1016/j.cbpb.2008.07.010, 18692590
    • Small BC, Murdock CA, Bilodeau-Bourgeois AL, Peterson BC, Waldbieser GC. Stability of reference genes for real-time PCR analyses in channel catfish (Ictalurus punctatus) tissues under varying physiological conditions. Comp Biochem Physiol B 2008, 151:296-304. 10.1016/j.cbpb.2008.07.010, 18692590.
    • (2008) Comp Biochem Physiol B , vol.151 , pp. 296-304
    • Small, B.C.1    Murdock, C.A.2    Bilodeau-Bourgeois, A.L.3    Peterson, B.C.4    Waldbieser, G.C.5
  • 18
    • 63749118892 scopus 로고    scopus 로고
    • Comparison of gene expression responses to hypoxia in viviparous (Xiphophorus) and oviparous (Oryzias) fishes using a medaka microarray
    • Boswell MG, Wells MC, Kirk LM, Ju Z, Zhang Z, Booth RE, et al. Comparison of gene expression responses to hypoxia in viviparous (Xiphophorus) and oviparous (Oryzias) fishes using a medaka microarray. Comp Biochem Physiol C 2009, 149:258-265.
    • (2009) Comp Biochem Physiol C , vol.149 , pp. 258-265
    • Boswell, M.G.1    Wells, M.C.2    Kirk, L.M.3    Ju, Z.4    Zhang, Z.5    Booth, R.E.6
  • 19
    • 38349041705 scopus 로고    scopus 로고
    • Transcriptome analysis of the response to chronic constant hypoxia in zebrafish hearts
    • 10.1007/s00360-007-0201-4, 2200676, 17828398
    • Marques IJ, Leito JTD, Spaink HP, Testerink J, Jaspers RT, Witte F, et al. Transcriptome analysis of the response to chronic constant hypoxia in zebrafish hearts. J Comp Physiol B 2008, 178:77-92. 10.1007/s00360-007-0201-4, 2200676, 17828398.
    • (2008) J Comp Physiol B , vol.178 , pp. 77-92
    • Marques, I.J.1    Leito, J.T.D.2    Spaink, H.P.3    Testerink, J.4    Jaspers, R.T.5    Witte, F.6
  • 20
    • 0036159220 scopus 로고    scopus 로고
    • The role of adenosine in the anoxic survival of the epaulette shark, Hemiscyllium ocellatum
    • 10.1016/S1096-4959(01)00484-5, 11818236
    • Renshaw GMC, Kerrisk CB, Nilsson GE. The role of adenosine in the anoxic survival of the epaulette shark, Hemiscyllium ocellatum. Comp Biochem Physiol B 2002, 131:133-141. 10.1016/S1096-4959(01)00484-5, 11818236.
    • (2002) Comp Biochem Physiol B , vol.131 , pp. 133-141
    • Renshaw, G.M.C.1    Kerrisk, C.B.2    Nilsson, G.E.3
  • 21
    • 0036164080 scopus 로고    scopus 로고
    • Exposure to hypoxia primes the respiratory and metabolic responses of the epaulette shark to progressive hypoxia
    • Routley MH, Nilsson GE, Renshaw GMC. Exposure to hypoxia primes the respiratory and metabolic responses of the epaulette shark to progressive hypoxia. Comp Biochem Physiol A 2002, 131:313-321.
    • (2002) Comp Biochem Physiol A , vol.131 , pp. 313-321
    • Routley, M.H.1    Nilsson, G.E.2    Renshaw, G.M.C.3
  • 22
    • 0242370148 scopus 로고    scopus 로고
    • Hypoxia induces adaptive and reversible gross morphological changes in crucian carp gills
    • 10.1242/jeb.00594, 12966058
    • Sollid J, De Angelis P, Gundersen K, Nilsson GE. Hypoxia induces adaptive and reversible gross morphological changes in crucian carp gills. J Exp Biol 2003, 206:3667-3673. 10.1242/jeb.00594, 12966058.
    • (2003) J Exp Biol , vol.206 , pp. 3667-3673
    • Sollid, J.1    De Angelis, P.2    Gundersen, K.3    Nilsson, G.E.4
  • 23
    • 0344026111 scopus 로고    scopus 로고
    • Brain blood flow and blood pressure during hypoxia in the epaulette shark Hemiscyllium ocellatum, a hypoxia-tolerant elasmobranch
    • Soderstrom V, Renshaw GMC, Nilsson GE. Brain blood flow and blood pressure during hypoxia in the epaulette shark Hemiscyllium ocellatum, a hypoxia-tolerant elasmobranch. J Exp Biol 1999, 202:829-835.
    • (1999) J Exp Biol , vol.202 , pp. 829-835
    • Soderstrom, V.1    Renshaw, G.M.C.2    Nilsson, G.E.3
  • 25
    • 0037129827 scopus 로고    scopus 로고
    • Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
    • Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 2002, 3:research0034.1-0034.11.
    • (2002) Genome Biol , vol.3
    • Vandesompele, J.1    De Preter, K.2    Pattyn, F.3    Poppe, B.4    Van Roy, N.5    De Paepe, A.6
  • 26
    • 3442876969 scopus 로고    scopus 로고
    • Normalization of real-time quantitative reverse transcription-PCR data: A model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets
    • 10.1158/0008-5472.CAN-04-0496, 15289330
    • Andersen CL, Jensen JL, Orntoft TF. Normalization of real-time quantitative reverse transcription-PCR data: A model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res 2004, 64:5245-5250. 10.1158/0008-5472.CAN-04-0496, 15289330.
    • (2004) Cancer Res , vol.64 , pp. 5245-5250
    • Andersen, C.L.1    Jensen, J.L.2    Orntoft, T.F.3
  • 27
    • 33746366455 scopus 로고    scopus 로고
    • Construction of a nurse shark (Ginglymostoma cirratum) bacterial artificial chromosome (BAC) library and a preliminary genome survey
    • 10.1186/1471-2164-7-106, 1513397, 16672057
    • Luo MZ, Kim HR, Kudrna D, Sisneros NB, Lee SJ, Mueller C, et al. Construction of a nurse shark (Ginglymostoma cirratum) bacterial artificial chromosome (BAC) library and a preliminary genome survey. BMC Genomics 2006, 7:106. 10.1186/1471-2164-7-106, 1513397, 16672057.
    • (2006) BMC Genomics , vol.7 , pp. 106
    • Luo, M.Z.1    Kim, H.R.2    Kudrna, D.3    Sisneros, N.B.4    Lee, S.J.5    Mueller, C.6
  • 29
    • 2542433065 scopus 로고    scopus 로고
    • Oxygen sensors and energy sensors act synergistically to achieve a graded alteration in gene expression: Consequences for assessing the level of neuroprotection in response to stressors
    • 10.2741/1206, 14766351
    • Renshaw GMC, Warburton J, Girjes A. Oxygen sensors and energy sensors act synergistically to achieve a graded alteration in gene expression: Consequences for assessing the level of neuroprotection in response to stressors. Front Biosci 2004, 9:110-116. 10.2741/1206, 14766351.
    • (2004) Front Biosci , vol.9 , pp. 110-116
    • Renshaw, G.M.C.1    Warburton, J.2    Girjes, A.3
  • 30
    • 0032716682 scopus 로고    scopus 로고
    • Moderate hypoxia increases heat shock protein 90 expression in excised rat aorta
    • 10.1159/000025675, 10559676
    • Almgren CM, Olson LE. Moderate hypoxia increases heat shock protein 90 expression in excised rat aorta. J Vasc Res 1999, 36:363-371. 10.1159/000025675, 10559676.
    • (1999) J Vasc Res , vol.36 , pp. 363-371
    • Almgren, C.M.1    Olson, L.E.2
  • 31
    • 33845972658 scopus 로고    scopus 로고
    • Induction of the heat shock pathway during hypoxia requires regulation of heat shock factor by hypoxia-inducible factor-1
    • 10.1074/jbc.M608013200, 17040902
    • Baird NA, Turnbull DW, Johnson EA. Induction of the heat shock pathway during hypoxia requires regulation of heat shock factor by hypoxia-inducible factor-1. J Biol Chem 2006, 281:38675-38681. 10.1074/jbc.M608013200, 17040902.
    • (2006) J Biol Chem , vol.281 , pp. 38675-38681
    • Baird, N.A.1    Turnbull, D.W.2    Johnson, E.A.3
  • 32
    • 0346728757 scopus 로고    scopus 로고
    • Guideline to reference gene selection for quantitative real-time PCR
    • 10.1016/j.bbrc.2003.11.177, 14706621
    • Radonic A, Thulke S, Mackay IM, Landt O, Siegert W, Nitsche A. Guideline to reference gene selection for quantitative real-time PCR. Biochem Biophys Res Commun 2004, 313:856-862. 10.1016/j.bbrc.2003.11.177, 14706621.
    • (2004) Biochem Biophys Res Commun , vol.313 , pp. 856-862
    • Radonic, A.1    Thulke, S.2    Mackay, I.M.3    Landt, O.4    Siegert, W.5    Nitsche, A.6
  • 33
    • 57049147881 scopus 로고    scopus 로고
    • Characterization of housekeeping genes in zebrafish: male-female differences and effects of tissue type, developmental stage and chemical treatment
    • 10.1186/1471-2199-9-102, 2588455, 19014500
    • McCurley AT, Callard GV. Characterization of housekeeping genes in zebrafish: male-female differences and effects of tissue type, developmental stage and chemical treatment. BMC Mol Biol 2008, 9:102. 10.1186/1471-2199-9-102, 2588455, 19014500.
    • (2008) BMC Mol Biol , vol.9 , pp. 102
    • McCurley, A.T.1    Callard, G.V.2
  • 34
    • 28344455318 scopus 로고    scopus 로고
    • Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmon
    • 10.1186/1471-2199-6-21, 1314898, 16293192
    • Olsvik PA, Lie KK, Jordal AEO, Nilsen TO, Hordvik I. Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmon. BMC Mol Biol 2005, 6:21. 10.1186/1471-2199-6-21, 1314898, 16293192.
    • (2005) BMC Mol Biol , vol.6 , pp. 21
    • Olsvik, P.A.1    Lie, K.K.2    Jordal, A.E.O.3    Nilsen, T.O.4    Hordvik, I.5
  • 35
    • 41449097016 scopus 로고    scopus 로고
    • Selection of housekeeping genes for gene expression studies in larvae from flatfish using real-time PCR
    • 10.1186/1471-2199-9-28, 2275743, 18325098
    • Infante C, Matsuoka MP, Asensio E, Canavate JP, Reith M, Manchado M. Selection of housekeeping genes for gene expression studies in larvae from flatfish using real-time PCR. BMC Mol Biol 2008, 9:28. 10.1186/1471-2199-9-28, 2275743, 18325098.
    • (2008) BMC Mol Biol , vol.9 , pp. 28
    • Infante, C.1    Matsuoka, M.P.2    Asensio, E.3    Canavate, J.P.4    Reith, M.5    Manchado, M.6
  • 36
    • 33846799231 scopus 로고    scopus 로고
    • Multiple tissue gene expression analyses in Japanese medaka (Oryzias latipes) exposed to hypoxia
    • Ju ZL, Wells MC, Heater SJ, Walter RB. Multiple tissue gene expression analyses in Japanese medaka (Oryzias latipes) exposed to hypoxia. Comp Biochem Physiol C 2007, 145:134-144.
    • (2007) Comp Biochem Physiol C , vol.145 , pp. 134-144
    • Ju, Z.L.1    Wells, M.C.2    Heater, S.J.3    Walter, R.B.4
  • 37
    • 0035161457 scopus 로고    scopus 로고
    • Unsuitability of using ribosomal RNA as loading control for Northern blot analyses related to the imbalance between messenger and ribosomal RNA content in rat mammary tumors
    • 10.1006/abio.2000.4889, 11141312
    • Solanas M, Moral R, Escrich E. Unsuitability of using ribosomal RNA as loading control for Northern blot analyses related to the imbalance between messenger and ribosomal RNA content in rat mammary tumors. Anal Biochem 2001, 288:99-102. 10.1006/abio.2000.4889, 11141312.
    • (2001) Anal Biochem , vol.288 , pp. 99-102
    • Solanas, M.1    Moral, R.2    Escrich, E.3
  • 38
    • 40549105489 scopus 로고    scopus 로고
    • Reference genes for normalization of gene expression studies in human osteoarthritic articular cartilage
    • 10.1186/1471-2199-9-17, 2248200, 18226276
    • Pombo-Suarez M, Calaza M, Gomez-Reino JJ, Gonzalez A. Reference genes for normalization of gene expression studies in human osteoarthritic articular cartilage. BMC Mol Biol 2008, 9:17. 10.1186/1471-2199-9-17, 2248200, 18226276.
    • (2008) BMC Mol Biol , vol.9 , pp. 17
    • Pombo-Suarez, M.1    Calaza, M.2    Gomez-Reino, J.J.3    Gonzalez, A.4
  • 39
    • 1842483281 scopus 로고    scopus 로고
    • Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper - Excel-based tool using pair-wise correlations
    • 10.1023/B:BILE.0000019559.84305.47, 15127793
    • Pfaffl MW, Tichopad A, Prgomet C, Neuvians TP. Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper - Excel-based tool using pair-wise correlations. Biotechnol Lett 2004, 26:509-515. 10.1023/B:BILE.0000019559.84305.47, 15127793.
    • (2004) Biotechnol Lett , vol.26 , pp. 509-515
    • Pfaffl, M.W.1    Tichopad, A.2    Prgomet, C.3    Neuvians, T.P.4
  • 40
    • 0032079463 scopus 로고    scopus 로고
    • Hypoxia tolerance in the epaulette shark (Hemiscyllium ocellatum)
    • Wise G, Mulvey JM, Renshaw GMC. Hypoxia tolerance in the epaulette shark (Hemiscyllium ocellatum). J Exp Zool 1998, 281:1-5.
    • (1998) J Exp Zool , vol.281 , pp. 1-5
    • Wise, G.1    Mulvey, J.M.2    Renshaw, G.M.C.3
  • 41
    • 0034683228 scopus 로고    scopus 로고
    • Neuronal oxidative hypometabolism in the brainstem of the epaulette shark (Hemiscyllium ocellatum) in response to hypoxic pre-conditioning
    • 10.1016/S0304-3940(00)01321-5, 10925160
    • Mulvey JM, Renshaw GMC. Neuronal oxidative hypometabolism in the brainstem of the epaulette shark (Hemiscyllium ocellatum) in response to hypoxic pre-conditioning. Neurosci Lett 2000, 290:1-4. 10.1016/S0304-3940(00)01321-5, 10925160.
    • (2000) Neurosci Lett , vol.290 , pp. 1-4
    • Mulvey, J.M.1    Renshaw, G.M.C.2
  • 42
    • 0033990048 scopus 로고    scopus 로고
    • Primer3 on the WWW for general users and for biologist programmers
    • Totowa, NJ: Humana Press, Krawetz S, Misener S
    • Rozen S, Skaletsky HJ. Primer3 on the WWW for general users and for biologist programmers. Bioinformatics Methods and Protocols: Methods in Molecular Biology 2000, 365-386. Totowa, NJ: Humana Press, Krawetz S, Misener S.
    • (2000) Bioinformatics Methods and Protocols: Methods in Molecular Biology , pp. 365-386
    • Rozen, S.1    Skaletsky, H.J.2


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