Misconceptions over Förster resonance energy transfer between proteins and ANS/bis-ANS: Direct excitation dominates dye fluorescence

Analytical Biochemistry

Volumn 401, Issue 1, 2010, Pages 99-106

  Hawe, Andrea ^a   Poole, Robert ^a   Jiskoot, Wim ^a  

^a LEIDEN UNIVERSITY   (Netherlands)

Author keywords

ANS; Bis ANS; Extrinsic dyes; Fluorescence spectroscopy; FRET; IgG; Protein; Static and dynamic quenching

Indexed keywords

AMINO ACIDS; DYNAMICS; EMISSION SPECTROSCOPY; ENERGY TRANSFER; FLUORESCENCE SPECTROSCOPY; FORSTER RESONANCE ENERGY TRANSFER; MAMMALS; QUENCHING;

FLUORESCENCE EMISSION SPECTRA; FRET; PROTEIN CHARACTERIZATION; RESONANCE ENERGY TRANSFER; STATIC AND DYNAMIC QUENCHING; STEADY STATE FLUORESCENCES; TIME-RESOLVED FLUORESCENCE; ULTRAVIOLET ABSORPTION;

PROTEINS;

4,4' DIANILINO 1,1' BINAPHTHYL 5,5' DISULFONIC ACID DIPOTASSIUM SALT; 8 ANILINO 1 NAPHTHALENESULFONIC ACID; ALCOHOL; BOVINE SERUM ALBUMIN; BUFFER; FLUORESCENT DYE; IMMUNOGLOBULIN G; N ACETYLTRYPTOPHAN; PROTEIN; TRYPTOPHAN; TYROSINE; UNCLASSIFIED DRUG;

ARTICLE; CONCENTRATION RESPONSE; CONTROLLED STUDY; FLUORESCENCE ANALYSIS; FLUOROMETRY; FORSTER RESONANCE ENERGY TRANSFER; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN INTERACTION; STEADY STATE; ULTRAVIOLET SPECTROSCOPY;

BOVINAE;

EID: 77951093406     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.02.031     Document Type: Article

References (32)

1. Hawe A., Sutter M., Jiskoot W. Extrinsic fluorescent dyes as tools for protein characterization. Pharm. Res. 2008, 25:1487-1499.
2. Cardamone M., Puri N.K. Spectrofluorimetric assessment of the surface hydrophobicity of proteins. Biochem. J. 1992, 282:589-593.
3. Togashi D.M., Ryder A.G. A fluorescence analysis of ANS bound to bovine serum albumin: binding properties revisited by using energy transfer. J. Fluoresc. 2008, 18:519-526.
4. Cocco M.J., Lecomte J.T. The native state of apomyoglobin described by proton NMR spectroscopy: interaction with the paramagnetic probe HyTEMPO and the fluorescent dye ANS. Protein Sci. 1994, 3:267-281.
5. Stryer L. The interaction of a naphthalene dye with apomyoglobin and apohemoglobin: a fluorescent probe of non-polar binding sites. J. Mol. Biol. 1965, 13:482-495.
6. Capelle M.A., Gurny R., Arvinte T. High throughput screening of protein formulation stability: practical considerations. Eur. J. Pharm. Biopharm. 2007, 65:131-148.
7. Capelle M.A., Gurny R., Arvinte T. A high throughput protein formulation platform: case study of salmon calcitonin. Pharm. Res. 2009, 26:118-128.
8. Chang T.L., Cheung H.C. A model for molecules with twisted intramolecular charge-transfer characteristics: solvent polarity effect on the nonradiative rates of dyes in a series of water ethanol mixed-solvents. Chem. Phys. Lett. 1990, 173:343-348.
9. Das K., Sarkar N., Nath D., Bhattacharyya K. Nonradiative pathways of anilino-naphthalene sulfonates: twisted intramolecular charge-transfer versus intersystem crossing. Spectrochim. Acta A 1992, 48:1701-1705.
10. Kavitha M., Sultan N.A., Swamy M.J. Fluorescence studies on the interaction of hydrophobic ligands with Momordica charantia (bitter gourd) seed lectin. J. Photochem. Photobiol. B 2009, 94:59-64.
11. Sarkar P., Bharill S., Gryczynski I., Gryczynski Z., Nair M.P., Lacko A.G. Binding of 8-anilino-1-naphthalenesulfonate to lecithin:cholesterol acyltransferase studied by fluorescence techniques. J. Photochem. Photobiol. B 2008, 92:19-23.
12. Sahu K., Mondal S.K., Ghosh S., Roy D., Bhattacharyya K. Temperature dependence of solvation dynamics and anisotropy decay in a protein: ANS in bovine serum albumin. J. Chem. Phys. 2006, 124:124909.
13. Chakraborty A., Basak S. PH-induced structural transitions of caseins. J. Photochem. Photobiol. B 2007, 87:191-199.
14. Takashi R., Tonomura Y., Morales M.F. 4,4′-Bis (1-anilinonaphthalene 8-sulfonate) (bis-ANS): a new probe of the active site of myosin. Proc. Natl. Acad. Sci. USA 1977, 74:2334-2338.
15. Vekshin N.L. Efficiency of energy transfer by resonance in quenching of protein fluorescence by dyes. J. Appl. Spectrosc. 1998, 65:4.
16. Sharma K.K., Kaur H., Kumar G.S., Kester K. Interaction of 1,1′-bi(4-anilino)naphthalene-5,5′-disulfonic acid with α-crystallin. J. Biol. Chem. 1998, 273:8965-8970.
17. Murali J., Jayakumar R. Spectroscopic studies on native and protofibrillar insulin. J. Struct. Biol. 2005, 150:180-189.
18. Yin H., Zhou Q., Panda M., Yeh L.C., Zavala M.C., Lee J.C. A fluorescence study of type I and type II receptors of bone morphogenetic proteins with bis-ANS (4,4′-dianilino-1,1′-bisnaphthyl-5,5′-disulfonic acid). Biochim. Biophys. Acta 2007, 1774:493-501.
19. Hawe A., Friess W., Sutter M., Jiskoot W. Online fluorescent dye detection method for the characterization of immunoglobulin G aggregation by size exclusion chromatography and asymmetrical flow field flow fractionation. Anal. Biochem. 2008, 378:115-122.
20. Hawe A., Kasper J.C., Friess W., Jiskoot W. Structural properties of monoclonal antibody aggregates induced by freeze-thawing and thermal stress. Eur. J. Pharm. Sci. 2009, 38:79-87.
21. Pace C.N., Vajdos F., Fee L., Grimsley G., Gray T. How to measure and predict the molar absorption coefficient of a protein. Protein Sci. 1995, 4:2411-2423.
22. Yu X.C., Margolin W. Inhibition of assembly of bacterial cell division protein FtsZ by the hydrophobic dye 5,5′-bis-(8-anilino-1-naphthalenesulfonate). J. Biol. Chem. 1998, 273:10216-10222.
23. Weber G., Young L.B. Fragmentation of bovine serum albumin by pepsin: II. isolation, amino acid composition, and physical properties of the fragments. J. Biol. Chem. 1964, 239:1424-1431.
24. Cumper C.W.N., Singleto A. Ultraviolet spectra of aniline 2-, 3-, and 4-aminopyridines and of some of their derivatives in n-hexane 1,4-dioxan ethanol and water solutions. J. Chem. Soc. B 1968, 649-651.
25. Klimtchuk E., Venyaminov S., Kurian E., Wessels W., Kirk W., Prendergast F.G. Photophysics of ANS: I. Protein-ANS complexes-intestinal fatty acid binding protein and single-Trp mutants. Biophys. Chem. 2007, 125:1-12.
26. Malicka J., Gryczynski I., Kusba J., Shen Y., Lakowicz J.R. Effects of metallic silver particles on resonance energy transfer in labeled bovine serum albumin. Biochem. Biophys. Res. Commun. 2002, 294:886-892.
27. Lakowicz J.R. Principles of Fluorescence Spectroscopy 2006, Springer, New York.
28. Patel A.B., Srivastava S., Phadke R.S. Interaction of 7-hydroxy-8-(phenylazo)1,3-naphthalenedisulfonate with bovine plasma albumin. J. Biol. Chem. 1999, 274:21755-21762.
29. Gentili P.L., Ortica F., Favaro G. Static and dynamic interaction of a naturally occurring photochromic molecule with bovine serum albumin studied by UV-visible absorption and fluorescence spectroscopy. J. Phys. Chem. B 2008, 112:16793-16801.
30. Peters T. Serum albumin. Adv. Protein Chem. 1985, 37:161-245.
31. Celej M.S., Montich G.G., Fidelio G.D. Protein stability induced by ligand binding correlates with changes in protein flexibility. Protein Sci. 2003, 12:1496-1506.
32. Matulis D., Lovrien R. 1-Anilino-8-naphthalene sulfonate anion-protein binding depends primarily on ion pair formation. Biophys. J. 1998, 74:422-429.