LysK, the enzyme lysing Staphylococcus aureus cells: Specific kinetic features and approaches towards stabilization

Biochimie

Volumn 92, Issue 5, 2010, Pages 507-513

  Filatova, Lyubov Yu ^a   Becker, Stephen C ^b   Donovan, David M ^b   Gladilin, Alexander K ^a   Klyachko, N L ^a  

^a MOSCOW STATE UNIVERSITY   (Russian Federation)

^b AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

Bacteriophage peptidoglycan hydrolase; Enzyme stabilization; LysK endolysin; Staphylococci; Thermal inactivation of recombinant enzyme

Indexed keywords

ANTIINFECTIVE AGENT; CALCIUM ION; CYSTEINE; EDETIC ACID; ENZYME; ENZYME LYSK; GLYCEROL; METAL ION; POLYOL; SORBITOL; SUCROSE; TREHALOSE; UNCLASSIFIED DRUG; WATER;

ARTICLE; BACTERIAL CELL; CATALYSIS; CELL AGGREGATION; CIRCULAR DICHROISM; COMPUTER MODEL; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CYTOLYSIS; ELECTROPHORESIS; ENERGY; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME STABILITY; ENZYME SUBSTRATE COMPLEX; IONIZATION; MICHAELIS MENTEN KINETICS; NONHUMAN; PH; PROTEIN SECONDARY STRUCTURE; SEDIMENTATION; STAPHYLOCOCCUS AUREUS; TEMPERATURE;

AMIDOHYDROLASES; BACTERIAL PROTEINS; CIRCULAR DICHROISM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; HOT TEMPERATURE; HYDROGEN-ION CONCENTRATION; ISOELECTRIC FOCUSING; KINETICS; MOLECULAR WEIGHT; STAPHYLOCOCCUS AUREUS;

STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS;

EID: 77951024306     PISSN: 03009084     EISSN: 61831638     Source Type: Journal    
DOI: 10.1016/j.biochi.2010.01.026     Document Type: Article

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