Examination of electron stains as a substitute for uranyl acetate for the ultrathin sections of bacterial cells

Journal of Electron Microscopy

Volumn 59, Issue 2, 2010, Pages 113-118

  Yamaguchi, Kaoru ^a   Suzuki, Ken Ichiro ^a   Tanaka, Kenji ^a  

^a NATIONAL INSTITUTE OF TECHNOLOGY AND EVALUATION   (Japan)

Author keywords

Bacteria; Electron staining; Freeze substitution; Oolong tea extract; Platinum blue; Potassium permanganate

Indexed keywords

LEAD; ORGANOMETALLIC COMPOUND; PERMANGANATE POTASSIUM; PHOSPHOTUNGSTIC ACID; PLATINUM; URANYL ACETATE;

ARTICLE; BACILLUS CEREUS; ELECTRON; ELECTRON MICROSCOPY; ESCHERICHIA COLI; EVALUATION; FREEZE DRYING; HISTOLOGY; METABOLISM; METHODOLOGY; MICROTOMY; STAINING; ULTRASTRUCTURE;

BACILLUS CEREUS; ELECTRONS; ESCHERICHIA COLI; FREEZE SUBSTITUTION; LEAD; MICROSCOPY, ELECTRON; MICROTOMY; ORGANOMETALLIC COMPOUNDS; PHOSPHOTUNGSTIC ACID; PLATINUM; POTASSIUM PERMANGANATE; STAINING AND LABELING; TISSUE EMBEDDING;

BACILLUS CEREUS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; POSIBACTERIA;

BACILLUS CEREUS; BACTERIOLOGY; CELL MEMBRANES; ELECTRON MICROSCOPES; ELECTRON MICROSCOPY; ELECTRONS; ESCHERICHIA COLI; LEAD COMPOUNDS; POTASH; URANIUM COMPOUNDS;

CYTOPLASMIC MEMBRANE; ELECTRON STAINING; FREEZE SUBSTITUTION; OOLONG TEA; OOLONG TEA EXTRACT; PHOSPHOTUNGSTIC ACID; PLATINUM BLUE; TEA EXTRACTS; ULTRA-THIN; URANYL ACETATE;

MANGANESE COMPOUNDS;

EID: 77951009916     PISSN: 00220744     EISSN: 14779986     Source Type: Journal    
DOI: 10.1093/jmicro/dfp045     Document Type: Article

References (16)

1. Bray D F and Wagenaar E B (1978) A double staining technique for improved contrast of thin sections from Spurr-embedded tissue. Can. J. Bot. 56: 129-132.
2. Yasutake S, Murakami M, and Yoshida N (1961) Double staining of tissue sections for electron microscopy with heavy metals (KMnO4 and Pb(CH3COO)2). J. Electron Microsc. 10: 233-237.
3. Watson M L (1958) Staining of tissue sections for electron microscopy with heavy metals. J. Biophys. Biochem. Cytol. 4: 475-478.
4. Inaga S, Katsumoto T, Tanaka K, Kameie T, Nakane H, and Naguro T (2007) Platinum blue as an alternative to uranyl acetate for staining in transmission electron microscopy. Arch. Histol. Cytol. 70: 43-49. (Pubitemid 46917492)
5. Sato S, Adachi A, Sasaki Y, and Ghazizadeh M (2007) Oolong tea extract as a substitute for uranyl acetate in staining of ultrathin sections. J. Microsc. 229: 17-20. (Pubitemid 350292350)
6. Spurr A R (1969) A low viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26: 31-43.
7. Sato S, Sasaki Y, Adachi A, Dai W, and Liu X-L (2003) Use of oolong tea extract (OTE) for elastin staining and enhancement in ultrathin sections. Med. Electron Microsc. 36: 179-182. (Pubitemid 37288279)
8. Reedy M K (1965) Section staining for electron microscopy. Incompatibility of methyl nadic anhydride with permanganates. J. Cell Biol. 26: 309-311.
9. Reynold E S (1963) The use of lead citrate at high pH as an electronopaque strain in electron microscopy. J. Cell Biol. 17: 208-212.
10. Amako K, Murata K, and Umeda A (1983) Structure of the envelope of Escherichia coli observed by the rapid-freezing and substitution fixation method. Microbiol. Immunol. 27: 95-99. (Pubitemid 13080376)
11. Amako K and Takade A (1985) The fine structure of Bacillus subtilis revealed by the rapid-freezing and substitution-fixation method. J. Electron Microsc. 34: 13-17. (Pubitemid 15023746)
12. Knight D P and Lewis P R (1992) Cytological staining methods, other sytological stains, other non-specific staining methods. In: Lewis P R and Knight D P (eds), Cytochemical Staining Methods for Electron Microscopy, pp 64, 65 (Elsevier, Amsterdam).
13. Luft J H (1956) Permanganate; a new fixative for electron microscopy. J. Biophys. Biochem. Cytol. 2: 799-802.
14. Mollenhauer H H (1959) Permanganate fixation of plant cells. J. Biophys. Biochem. Cytol. 6: 431-436.
15. Richardson W D and Davies H G (1980) Quantitative observations on the kinetics and mechanisims of binding of electron stains to thin sections through hen erythrocytes. J. Cell Sci. 46: 253-278.
16. Silverman L and Glick D (1969) The reactivity and staining of tissue proteins with phosphotungstic acid. J. Cell Boil. 40: 761-767.