Differential expression of defence-related proteins in Vigna unguiculata (L. Walp.) seedlings after infection with Fusarium oxysporum

Crop Protection

Volumn 29, Issue 5, 2010, Pages 440-447

  Vieira, Felipe A ^a   Carvalho, André O ^a   Vitória, Ângela P ^a   Retamal, Claudio A ^a   Gomes, Valdirene M ^a  

^a UNIVERSIDADE ESTADUAL DO NORTE FLUMINENSE DARCY RIBEIRO   (Brazil)

Author keywords

1,3 glucanase; Fusarium oxysporum; LTP; Peroxidase; Seedling; Vigna unguiculata

Indexed keywords

BIOASSAY; ELECTROKINESIS; ENZYME ACTIVITY; EXPERIMENTAL STUDY; FUNGUS; GENE EXPRESSION; LEGUME; MORPHOMETRY; PROTEIN; SEEDLING;

BACTERIA (MICROORGANISMS); FUNGI; FUSARIUM OXYSPORUM; VIGNA UNGUICULATA;

EID: 77950916623     PISSN: 02612194     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cropro.2009.10.010     Document Type: Article

References (40)

1. Arondel V., Vergnolle C., Cantrem C., and Kader J.-C. Lipid transfer proteins are encoded by a small multigene family in Arabidopsis thaliana. Plant Sci. 157 (2000) 1-12
2. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of dye binding. Anal. Biochem. 72 (1976) 248-254
3. Caruso C., Chilosi G., Caporale C., Leonardi L., Bertini L., Magro P., and Buonocore V. Induction of pathogenesis-related proteins in germinating wheat seeds infected with Fusarium culmorum. Plant Sci. 140 (1999) 87-97
4. Caruso C., Chilosi G., Leonardi L., Bertini L., Magro P., Buonocore V., and Caporale C. A basic peroxidase from wheat kernel with antifungal activity. Phytochemistry 58 (2001) 43-50
5. Carvalho A.O., Machado O.L.T., Da Cunha M., Santos I.S., and Gomes V.M. Antimicrobial peptides and immunolocalization of a LTP in Vigna unguiculata seeds. Plant Physiol. Biochem. 39 (2001) 137-146
6. Carvalho A.O., Teodoro C.E.S., Da Cunha M., Okorokova-Façanha A.L., Okorokov L.A., Fernandes K.V.S., and Gomes V.M. Intracellular localization of a lipid transfer protein in Vigna unguiculata seeds. Physiol. Plantarum 122 (2004) 328-336
7. Carvalho A.O., and Gomes V.M. Role of plant lipid transfer proteins in plant cell physiology - a concise review. Peptides 28 (2007) 1144-1153
8. Durrant W.E., and Dong X. Systemic acquired resistance. Annu. Rev. Phytopathol. 42 (2004) 185-209
9. Fatokun, C.A., Tarawali, S.A., Singh, B.B., Kormawa, P.M., Tamò, M., 2002. Challenges and opportunities for enhancing sustainable cowpea production. In: Proceedings of the World Cowpea Conference III, International Institute for Tropical Agriculture, Ibadan, Nigeria, p. 433.
10. Ferreira R.B., Monteiro S., Freitas R., Santos C.N., Chen Z., Batista L.M., Duarte J., Borges A., and Teixeira A.R. The role of plant defence proteins in fungal pathogenesis. Mol. Plant Pathol. 8 (2007) 677-700
11. Fink W., Liefland M., and Mendgen K. Chitinases and β-1,3-glucanases in the apoplastic compartment of oat leaves (Avena sativa L.). Plant Physiol. 88 (1988) 270-275
12. Funnell D.L., Lawrence C.B., Pedersen J.F., and Schardl C.L. Expression of the tobacco β-1,3-glucanase gene, PR-2d, following induction of SAR with Peronospora tabacina. Physiol. Mol. Plant Pathol. 65 (2004) 285-296
13. Gomes V.M., Oliveira A.E.A., and Xavier-Filho J. A chitinase and a β-1,3-glucanase isolated from the seeds of cowpea (Vigna unguiculata L. Walp.) inhibit the growth of fungi and insect pests of the seed. J. Sci. Food Agric. 72 (1996) 86-90
14. Granier F. Extraction of plant proteins for two-dimensional electrophoresis. Electrophoresis 9 (1998) 712-718
15. Houterman P.M., Speijer D., Dekker H.L., De Koster C.G., and Cornelissen B.J.C. The mixed xylem sap proteome of Fusarium oxysporum-infected tomato plants. Mol. Plant Pathol. 8 (2007) 215-221
16. Jang C.S., Lee H.J., Chang S.J., and Seo Y.W. Expression and promoter analysis of the TaLTP1 gene induced by drought and salt stress in wheat (Triticum aestivum L.). Plant Sci. 167 (2004) 995-1001
17. Ji C., and Kuć J. Antifungal activity of cucumber β-1,3-glucanase and chitinase. Physiol. Mol. Plant Pathol. 49 (1996) 257-265
18. Jin X., Feng D., Wang H., and Wang J. A novel tissue-specific plantain β-1,3-glucanase gene that is regulated in response to infection by Fusarium oxysporum f. sp. cubense. Biotechnol. Lett. 29 (2007) 1431-1437
19. Jung H.W., Kim W., and Hwang B.K. Three pathogen-inducible genes encoding lipid transfer protein from pepper are differentially activated by pathogens, abiotic, and environmental stresses. Plant Cell Environ. 26 (2003) 915-928
20. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
21. Lamb C., and Dixon R.A. The oxidative burst in plant disease resistance. Annu. Rev. Plant Physiol. Plant Mol. Biol. 48 (1997) 251-275
22. Leon J.C., Alpeeva I.S., Chubar T.A., Galaev I.Y., Csoregi E., and Sakharov I.Y. Purification and substrate specificity of peroxidase from sweet potato tubers. Plant Sci. 163 (2002) 1011-1019
23. Molina A., Segura A., and Gracía-Olmedo F. Lipid transfer proteins (nsLTPs) from barley and maize leaves are potent inhibitors of bacterial and fungal plant pathogens. FEBS Lett. 316 (1993) 119-122
24. Nelson N. A photometric adaptation of the Somogy method of determination of glucose. J. Biol. Chem. 153 (1944) 375-380
25. Nóbrega F.M., Santos I.S., Da Cunha M., Carvalho A.O., and Gomes V.M. Antimicrobial proteins from cowpea root exudates: inhibitory activity against Fusarium oxysporum and purification of a chitinase-like protein. Plant Soil 272 (2005) 223-232
26. O'Brien M., and Colwell R.R. A rapid test for chitinase activity that uses 4-methylumberiferyl-N-acetyl-D-glucosamine. Appl. Environ. Microbiol. 7 (1987) 1718-1720
27. Pereira L.F., Goodwin P.H., and Erickson L. Peroxidase activity during susceptible and resistant interactions between cassava (Manihot esculenta) and Xanthomonas axonopodis pv. manihotis and Xanthomonas cassavae. J. Phytopathol. 148 (2000) 575-577
28. Phillips R.D., McWatters K.H., Chinnan M.S., Hung Y.C., Beuchat L.R., Sefa-Dedeh S., Sakyi-Dawson E., Ngoddy P., Nnanyelugo D., Enwere J., Komey N.S., Liu K., Mensa-Wilmot Y., Nnanna I.A., Okeke C., Prinyawiwatkul W., and Saalia F.K. Utilization of cowpeas for human food. Field Crops Res. 82 (2003) 193-213
29. Quiroga M., Guerrero C., Botella M.A., Barcelo A., Amaya I., Medina M.I., Alonso F.J., Forchetti S.M., Tigier H., and Valpuesta V. A tomato peroxidase involved in the synthesis of lignin and suberin. Plant Physiol. 122 (2000) 1119-1127
30. Rose T.L., Silva-Conceição A., Xavier-Filho J., Okorokov L.A., Fernandes K.V.S., Marty F., Marty-Mazars D., Carvalho A.O., and Gomes V.M. Defense proteins from Vigna unguiculata seeds exudates: characterization and inhibitory activity against Fusarium oxysporum. Plant Soil 286 (2006) 181-191
31. Santos I.S., Machado O.L.T., Da Cunha M., and Gomes V.M. A chitinase from Adenanthera pavonina L. seeds: purification, characterization and immunolocalization. Plant Sci. 167 (2004) 1203-1210
32. Schägger H., and von Jagow G. Tricine-sodium dodecylsulfate polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166 (1987) 368-379
33. Sels J., Mathys J., De Coninck B.M.A., Cammue B.P.A., and De Bolle M.F.C. Plant pathogenesis-related (PR) proteins: a focus on PR peptides. Plant Physiol. Biochem. 46 (2008) 941-950
34. Shimoni M. A method for activity staining of peroxidase and β-1,3-glucanase isozymes in polyacrylamide electrophoresis gels. Anal. Biochem. 220 (1994) 36-38
35. Somogy M. Notes on sugar determination. J. Biol. Chem. 195 (1952) 19-23
36. Sterk P., Booij H., Schellekens G.A., Van Kammen A., and De Vries S.C. Cell-specific expression of the carrot EP2 lipid transfer protein gene. Plant Cell 3 (1991) 907-921
37. Towbin H., Staehelin T., and Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. U S A 76 (1979) 4350-4354
38. Vignols F., Wigger M., García-Garrido J.M., Grellet F., Kader J.-C., and Delseny M. Rice lipid transfer protein (LTP) genes belong to a complex multigene family and are differentially regulated. Gene 195 (1997) 177-186
39. Wu C.T., Leubner-Metzger G., Meins Jr. F., and Bradford K.J. Class I β-1,3-glucanase and chitinase are expressed in the micropylar endosperm of tomato seeds prior to radicle emergence. Plant Physiol. 126 (2001) 1299-1313
40. Xavier-Filho J., Sales M.P., Fernandes K.V.S., and Gomes V.M. The resistance of cowpea (Vigna unguiculata) seeds to the cowpea weevil (Callosobruchus maculatus) is due to the association of variant vicilins (7S storage proteins) to chitinous structures in insect's midgut. Arq. Biol. Technol. 39 (1996) 693-699