Analysis of proteins: Introduction

Current Protocols in Molecular Biology

Volumn , Issue SUPPL. 90, 2010, Pages

  Scopes, R K ^a   Smith, John A ^b  

^a LA TROBE UNIVERSITY   (Australia)

^b UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMYLASE; CYTOPLASM PROTEIN; MEMBRANE PROTEIN; PROTEINASE; RECOMBINANT PROTEIN; TRIACYLGLYCEROL LIPASE;

BACTERIUM CULTURE; CELL CULTURE; CRYSTALLIZATION; EDITORIAL; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN ISOLATION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; PROTEOMICS; QUANTITATIVE ANALYSIS; STRUCTURAL GENOMICS;

EID: 77950845940     PISSN: 19343639     EISSN: 19343647     Source Type: Journal    
DOI: 10.1002/0471142727.mb1000s76     Document Type: Editorial

References (17)

1. Coligan, J.E., Dunn, B.M., Speicher, D.W., and, Wingfield, P.T., (eds.). 2006. Current Protocols in Protein Science. John Wiley & Sons, Hoboken, N.J..
2. Oka, M., Yang, Y.S., Nagata, S., Esaki, N., Tanaka, M., and, Soda, K., 1989. Overproduction of thermostable leucine dehydrogenase of Bacillus stearothermophilus and its one-step purification from recombinant cells of Escherichia coli. Biotechnol. Appl. Biochem. 11: 307 - 316.
3. Pain, R.H., 1995. Overview of protein folding. In Current Protocols in Protein Science (, J.E. Coligan, B.M. Dunn, H.L. Ploegh, D.W. Speicher, and, P.T. Wingfield, eds.) pp. 6.4.1 - 6.4.7. John Wiley & Sons, New York.
4. Palmer, I., and, Wingfield, P.T., 2004. Preparation and extraction of insoluble (inclusion-body) proteins from Escherichia coli. In Current Protocols in Protein Science (, J.E. Coligan, B.M. Dunn, D.W. Speicher, and, P.T. Wingfield, eds.) pp. 6.3.1 - 6.3.18. John Wiley & Sons, Hoboken, N.J.
5. Sassenfeld, H.M., 1990. Engineering proteins for purification. Trends Biotechnol. 8: 88 - 93.
6. Walter, H., and, Johansson, G., (eds.) 1994. Aqueous two-phase systems. Methods Enzymol. 228: 1 - 725.
7. Wingfield, P.T., 1995. Use of protein folding reagents. In Current Protocols in Protein Science (, J.E. Coligan, B.M. Dunn, D.W. Speicher, and, P.T. Wingfield, eds.) pp. A.3A.1 - A.3A.4. John Wiley & Sons, New York.
8. Wingfield, P.T., 2002. Overview of the purification of recombinant proteins produced in Escherichia coli. In Current Protocols in Protein Science (, J.E. Coligan, B.M. Dunn, H.L. Ploegh, D.W. Speicher, and, P.T. Wingfield, eds.) pp. 6.1.1 - 6.1.37. John Wiley & Sons, New York.
9. Wingfield, P.T., 2005. Preparation of soluble proteins from Escherichia coli. In Current Protocols in Protein Science (, J.E. Coligan, B.M. Dunn, D.W. Speicher, and, P.T. Wingfield, eds.) pp. 6.2.1 - 6.2.22. John Wiley & Sons, Hoboken, N.J.
10. Wingfield, P.T., Palmer, I., and, Liang, S.-M., 1995. Folding and purification of insoluble (inclusion-body) proteins from Escherichia coli. In Current Protocols in Protein Science (, J.E. Coligan, B.M. Dunn, H.L. Ploegh, D.W. Speicher, and, P.T. Wingfield, eds.) pp. 6.5.1 - 6.5.27. John Wiley & Sons, New York.
11. Zeilstra-Ryalls, J., Fayet, O., and, Georgopoulos, C., 1991. The universally-conserved GroE (Hsp60) chaperonins. Annu. Rev. Microbiol. 45: 301 - 325.
12. Coligan et al., 2006. See above. Companion to this manual giving detailed protocols for many procedures commonly used in protein chemistry, including general handling, purification, and analysis.
13. Deutscher, M.P., (ed.) 1990. Guide to protein purification. Methods Enzymol. 182: 1 - 894. Extensive collection of purification methods, with some general protocols and examples.
14. Janson, J.-C., and, Ryden, L.G., 1989. Protein Purification: Principles, High Resolution Methods, and Applications. VCH Publishers, New York. A useful collection of methods and examples.
15. Kennedy, J.F., and, Cabral, J.M., (eds.) 1993. Recovery Processes for Biological Materials. John Wiley & Sons, New York. A useful introduction to the problems of large-scale methods.
16. Kenny, A., and, Fowell, S., (eds.) 1992. Practical protein chromatography. Methods Mol. Biol. 11: 1 - 327. Extensive descriptions of affinity chromatographic techniques with protocols and recipes.
17. Scopes, R.K., 1993. Protein Purification, Principles and Practice, 3rd ed. Springer-Verlag, New York and Heidelberg. General principles of all the main techniques used in purifying proteins. A useful laboratory handbook; does not include recipes or procedures for specific proteins.