Dermacentor marginatus and Ixodes ricinus ticks versus L929 and Vero cell lines in Rickettsia slovaca life cycle evaluated by quantitative real time PCR

Experimental and Applied Acarology

Volumn 50, Issue 4, 2010, Pages 353-359

  Boldiš, Vojtech ^a   Špitalská, Eva ^a  

^a INSTITUTE OF VIROLOGY   (Slovakia)

Author keywords

Dermacentor marginatus; Ixodes ricinus; L929; Quantitative real time PCR; Rickettsia slovaca; Transmission electron microscopy; Vero

Indexed keywords

BACTERIAL DISEASE; BACTERIUM; INFECTIVITY; INOCULATION; LIFE CYCLE; POLYMERASE CHAIN REACTION; REAL TIME; TICK; TRANSMISSION ELECTRON MICROSCOPY;

ANIMAL; ARTICLE; CELL LINE; CERCOPITHECUS; DERMACENTOR; GROWTH, DEVELOPMENT AND AGING; IXODES; LIFE CYCLE; MICROBIOLOGY; MOUSE; PHYSIOLOGY; POLYMERASE CHAIN REACTION; RICKETTSIA; TRANSMISSION ELECTRON MICROSCOPY; VERO CELL;

ANIMALS; CELL LINE; CERCOPITHECUS AETHIOPS; DERMACENTOR; IXODES; LIFE CYCLE STAGES; MICE; MICROSCOPY, ELECTRON, TRANSMISSION; POLYMERASE CHAIN REACTION; RICKETTSIA; VERO CELLS;

CENTRAL EUROPE; MEDITERRANEAN REGION;

ACARI; ANIMALIA; BACTERIA (MICROORGANISMS); DERMACENTOR; DERMACENTOR MARGINATUS; DERMACENTOR RETICULATUS; IXODES RICINUS; IXODIDA; IXODIDAE; MAMMALIA; RICINUS COMMUNIS; RICKETTSIA; RICKETTSIA SLOVACA; RICKETTSIACEAE;

EID: 77950690220     PISSN: 01688162     EISSN: 15729702     Source Type: Journal    
DOI: 10.1007/s10493-009-9322-7     Document Type: Article

References (23)

1. Balashov YS (1972) Bloodsucking ticks (Ixodoidea)-vectors of diseases of man and animals. Misc Pub Ent Soc Amer 8: 5.
2. Beati L, Finidori JP, Raoult D (1993) First isolation of Rickettsia slovaca from Dermacentor marginatus in France. Am J Trop Med Hyg 48: 257-268.
3. Boldiš V, Kocianová E, Štrus J, Tušek-Žnidarič M, Sparagano O, Štefanidesová K, Špitalská E (2008) Rickettsial agents in Slovakian ticks (Acarina, Ixodidae) and their ability to grow in Vero and L929 cell lines. Ann NY Acad Sci 1149: 281-285.
4. Boldiš V, Štrus J, Kocianová E, Tušek-Žnidarič M, Štefanidesová K, Schwarzová K, Kúdelová M, Sekeyová Z, Špitalská E (2009a) Life cycle of Rickettsia slovaca in L929 cell line studied by quantitave real-time PCR and transmission electron microscopy. FEMS Lett 293: 102-106. doi: 10. 1111/j. 1574-6968. 2009. 01510. x.
5. Boldiš V, Štrus J, Kocianová E, Tušek-Žnidarič M, Štefanidesová K, Špitalská E (2009b) Ultrastructural studies on life cycle of Rickettsia slovaca, wild and standard type, cultivated in L929 and Vero cell lines. Folia Microbiol 54: 130-136.
6. Eremeeva ME, Dasch GA (2000) Rickettsiae. In: Lederberg J (ed) Encyclopedia of microbiology, 2nd edn. Academic Press, New York, pp 140-180.
7. Eremeeva ME, Bosserman EA, Demma LJ, Zambrano ML, Blau DM, Dasch GA (2006) Isolation and identification of Rickettsia massiliae from Rhipicephalus sanguineus ticks collected in Arizona. Appl Environ Microbiol 72: 5569-5577.
8. Fenollar F, Maurin M, Raoult D (2003) Wolbachia pipientis growth kinetics and susceptibilities to 13 antibiotics determined by immunofluorescence staining and real-time PCR. Antimicrob Agents Chemother 47: 1665-1671.
9. Harden VA (1990) Rocky mountain spotted fever: history of a twentieth-century disease. The Johns Hopkins University Press, Baltimore, Maryland.
10. Harley JP, Prescott LM (2002) Laboratory exercises in microbiology, 5th edn. McGraw-Hill, New York.
11. Hayes SF, Burgdorfer W (1989) Interactions between rickettsial endocytobionts and their tick hosts. In: Selwemmler W, Gassner G (eds) Insect endocytobiosis: morphology, physiology, genetics, evolution. CRC Press, Boca Raton, pp 235-251.
12. Korshus JB, Munderloh UG, Bey RG, Kurtti TJ (2004) Experimental infection of dogs with Borrelia burgdorferi sensu stricto using Ixodesscapularis ticks artificially infected by capillary feeding. Med Microbiol Immunol 193: 27-34.
13. Macaluso KR, Sonenshine DE, Ceraul SM, Azad AF (2002) Rickettsial infection in Dermacentor variabilis (Acari: Ixodidae) inhibits transovarial transmission of a second rickettsia. J Med Entomol 39: 808-813.
14. Munderloh UG, Kurtti TJ (1995) Cellular and molecular interrelationships between ticks and prokaryotic pathogens. Ann Rev Entomol 40: 221-243.
15. Regnery RL, Spruill CL, Plikaytis BD (1991) Genotypic identification of rickettsiae and estimation of intraspecies sequence divergence for portions of two rickettsial genes. J Bacteriol 173: 1576-1589.
16. Řeháček J (1984) Rickettsia slovaca, the organism and its ecology. Acta Sci Nat Acad Sci Bohemoslov Brno 18: 1-50.
17. Roux V, Fournier PE, Raoult D (1996) Differentiation of spotted fever group rickettsiae by sequencing and analysis of restriction fragment length polymorphism of PCR-amplified DNA of the gene encoding the protein rOmpA. J Clin Microbiol 34: 2058-2065.
18. Silverman DJ, CLJr Wisseman, Waddell AD, Jones M (1978) External layers of Rickettsia prowazekii and Rickettsia rickettsii: occurrence of a slime layer. Infect Immun 22: 233-246.
19. Špitalská E, Kocianová E, Výrosteková V (2002) Natural focus of Coxiella burnetii and rickettsiae of SFG in southwestern Slovakia. Biologia 57: 589-595.
20. Uchiyama T (2005) Growth of typhus group and spotted fever group rickettsiae in insect cells. Ann NY Acad Sci 1063: 215-221.
21. Wilson K (1995) Preparation of genomic DNA from bacteria. In: Ausubel MF, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds) Current protocols in molecular biology. John Wiley and Sons, New York, pp 2. 4. 1-2. 4. 5.
22. Zanetti AS, Pornwiroon W, Kearney MT, Macaluso KR (2008) Characterization of rickettsial infection in Amblyomma americanum (Acari: Ixodidae) by quantitative real-time polymerase chain reaction. J Med Entomol 45: 267-275.
23. Žnidaršič N, Štrus J, Drobne D (2003) Ultrastructural alterations of the hepatopancreas in Porcellio scaber under stress. Environ Toxicol Phar 13: 161-174.