Sample preparation for sequencing hits from one-bead-one-peptide combinatorial libraries by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Analytical Biochemistry

Volumn 400, Issue 2, 2010, Pages 295-297

  Martínez Ceron, María C ^a   Giudicessi, Silvana L ^b   Marani, Mariela M ^a   Albericio, Fernando ^c,d   Cascone, Osvaldo ^a   Erra Balsells, Rosa ^b   Camperi, Silvia A ^a  

^a UNIVERSIDAD DE BUENOS AIRES   (Argentina)

^b Universidad Nacional de Río Cuarto Consejo Nacional de Investigaciones Científicas y Técnicas   (Argentina)

^c INSTITUTE FOR RESEARCH IN BIOMEDICINE   (Spain)

^d UNIVERSITY OF BARCELONA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ADDITIVES; AMMONIA; AMMONIUM HYDROXIDE; DESORPTION; LIBRARIES; PEPTIDES;

CLUSTER FORMATIONS; COMBINATORIAL LIBRARY; CYANO-4-HYDROXYCINNAMIC ACIDS; DRIED-DROPLET METHOD; MATRIX-ASSISTED LASER DESORPTION/IONIZATION-TIME-OF-FLIGHT MASS SPECTROMETRY; PEPTIDE BEADS; SAMPLE PREPARATION; WHOLE PROCESS;

MASS SPECTROMETRY;

ACETIC ACID; GUANIDINE; TETRAHYDROFURAN; TRIFLUOROACETIC ACID; UREA;

AMBIENT AIR; ARTICLE; CRYSTALLIZATION; ELUTION; IONIZATION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PEPTIDE ANALYSIS; PRIORITY JOURNAL; PROCESS OPTIMIZATION; SENSITIVITY ANALYSIS; TANDEM MASS SPECTROMETRY;

EID: 77950689037     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.01.029     Document Type: Article

References (13)

1. Lam K.S., Salmon S.E., Hersh E.M., Hruby V.J., Kazmierski W.M. A new type of synthetic peptide library for identifying ligand-binding activity. Nature 1991, 354:82-84.
2. Lam K.S., Lehman A.L., Song A., Doan N., Enstrom A.M., Maxwell J., Liu R. Synthesis and screening of " one-bead one-compound" combinatorial peptide libraries. Methods in Enzymology 2003, vol. 369:298-322. Academic Press, San Diego.
3. Aina O.H., Liu R., Sutcliffe J.L., Marik J., Pan C.X., Lam K.S. From combinatorial chemistry to cancer-targeting peptides. Mol. Pharm. 2007, 4:631-651.
4. Camperi S.A., Iannucci N.B., Albanesi G.J., Oggero Eberhardt M., Etcheverrigaray M., Messeguer A., Albericio F., Cascone O. Monoclonal antibody purification by affinity chromatography with ligands derived from the screening of peptide combinatory libraries. Biotechnol. Lett. 2003, 25:1545-1548.
5. Camperi S.A., Marani M.M., Iannucci N.B., Côté S., Albericio F., Cascone O. An efficient strategy for the preparation of one-bead-one-peptide libraries on a new biocompatible solid support. Tetrahedron Lett. 2005, 46:1561-1564.
6. Marani M.M., Oliveira E., Côté S., Camperi S.A., Albericio F., Cascone O. Identification of protein-binding peptides by direct MALDI-TOF-MS analysis of peptide beads selected from the screening of one-bead-one-peptide combinatorial libraries. Anal. Biochem. 2007, 370:215-222.
7. Marani M.M., Martínez Ceron M.C., Giudicessi S.L., Oliveira E., Côté S., Erra-Balsells R., Albericio F., Cascone O., Camperi S.A. Screening of one-bead-one-peptide combinatorial library using red fluorescent dyes: presence of positive and false positive beads. J. Comb. Chem. 2009, 11:146-150.
8. Atherton E., Logan C.J., Sheppard R.C. Peptide synthesis: II. Procedures for solid-phase synthesis using N-α-fluorenylmethoxycarbonylamino-acids on polyamide supports: synthesis of substance P and of acyl carrier protein 65-74 decapeptide. J. Chem. Soc. Perkin Trans. 1981, 1:538-546.
9. Bray A.M., Valerio R.M., Maeji N.J. Cleavage of resin-bound peptide esters with ammonia vapour: simultaneous multiple synthesis of peptide amides. Tetrahedron Lett. 1993, 34:4411-4414.
10. García-Martín F., Quintanar-Audelo M., García-Ramos Y., Cruz L.J., Gravel C., Furic R., Côté S., Tulla-Puche J., Albericio F. ChemMatrix, a poly(ethylene glycol)-based support for the solid-phase synthesis of complex peptides. J. Comb. Chem. 2006, 8:213-220.
11. Nonami H., Fukui S., Erra-Balsells R. β-Carboline alkaloids as matrices for matrix-assisted ultraviolet laser desorption time-of-flight mass spectrometry of proteins and sulfated oligosaccharides: a comparative study using phenylcarbonyl compounds, carbazoles, and classical matrices. J. Mass Spectrom. 1997, 32:287-296.
12. Nishikaze T., Takayama M. Disappearance of interfering alkali-metal adducted peaks from matrix-assisted laser desorption/ionization mass spectra of peptides with serine addition to α-cyano-4-hydroxycinnamic acid matrix. Rapid Commun. Mass Spectrom. 2007, 21:3345-3351.
13. Zhu X., Papayannopoulos I.A. Improvement in the detection of low concentration protein digests on a MALDI TOF/TOF workstation by reducing α-cyano-4-hydroxycinnamic acid adduct ions. J. Biomol. Tech. 2003, 14:298-307.