Verification of protein disulfide bond arrangement by ingel tryptic digestion under entirely neutral pH conditions

Proteomics

Volumn 10, Issue 7, 2010, Pages 1505-1509

  Saito, Kazuki ^a,b   Yasuo, Ltsuki ^a   Uchimura, Hiromasa ^a   Koide Yoshida, Shizuyo ^a   Mizuguchi, Takaaki ^a   Kiso, Yoshiaki ^a  

^a KYOTO PHARMACEUTICAL UNIVERSITY   (Japan)

^b RIKEN   (Japan)

Author keywords

Disulfide bond arrangement; In gel tryptic digestion; Neuregulin egf like motif; Neutral sds page; Proteolytic 18o iabeling; Technology

Indexed keywords

CYSTEINE; NEU DIFFERENTIATION FACTOR; PROTEIN; TRYPSIN;

ARTICLE; DIGESTION; DISULFIDE BOND; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEOMICS;

AMINO ACID SEQUENCE; DISULFIDES; HYDROGEN-ION CONCENTRATION; ISOTOPE LABELING; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NEUREGULIN-1; OXYGEN ISOTOPES; PEPTIDE FRAGMENTS; PROTEIN CONFORMATION; PROTEOMICS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TRYPSIN;

EID: 77950675887     PISSN: 16159853     EISSN: 16159861     Source Type: Journal    
DOI: 10.1002/pmic.200900056     Document Type: Article

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