Assessment of housekeeping genes for use in normalization of real time PCR in skeletal muscle with chronic degenerative changes

Experimental and Molecular Pathology

Volumn 88, Issue 2, 2010, Pages 326-329

  Yuzbaolu A ^a   Onbalar, I ^a   Kocaefe, C ^a   OzguC M ^a  

^a HACETTEPE UNIVERSITY   (Turkey)

Author keywords

Beta actin; GAPDH; HRPT; Muscle degeneration; Real Time PCR; TBP

Indexed keywords

BETA ACTIN; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; TATA BINDING PROTEIN;

ACHILLES TENDON; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; GASTROCNEMIUS MUSCLE; GENE EXPRESSION; HOUSEKEEPING GENE; MALE; MUSCLE ATROPHY; NONHUMAN; NUCLEOTIDE SEQUENCE; RAT; REAL TIME POLYMERASE CHAIN REACTION; SKELETAL MUSCLE; SOLEUS MUSCLE; TENOTOMY;

ACHILLES TENDON; ACTINS; ANIMALS; GENE EXPRESSION PROFILING; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; MALE; MODELS, ANIMAL; MUSCLE, SKELETAL; MUSCULAR DISEASES; POLYMERASE CHAIN REACTION; RATS; RATS, SPRAGUE-DAWLEY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TATA-BOX BINDING PROTEIN;

RATTUS;

EID: 77950628180     PISSN: 00144800     EISSN: 10960945     Source Type: Journal    
DOI: 10.1016/j.yexmp.2009.12.007     Document Type: Article

References (15)

1. Bacou F., et al. Acetylcholinesterase forms in fast and slow rabbit muscle. Nature 1982, 296:661-664.
2. Batt J., et al. Differential gene expression profiling of short and long term denervated muscle. FASEB J. 2006, 20:115-117.
3. Bulfield G., et al. X chromosome-linked muscular dystrophy (mdx) in the mouse. Proc. Natl. Acad. Sci. U. S. A. 1984, 81:1189-1192.
4. Gerber C., et al. Effect of tendon release and delayed repair on the structure of the muscles of the rotator cuff: an experimental study in sheep. J. Bone Joint Surg. Am. 2004, 86-A:1973-1982.
5. Hall-Craggs E.C., Seyan H.S. Histochemical changes in innervated and denervated skeletal muscle fibers following treatment with bupivacaine (marcain). Exp. Neurol. 1975, 46:345-354.
6. Jemiolo B., Trappe S. Single muscle fiber gene expression in human skeletal muscle: validation of internal control with exercise. Biochem. Biophys. Res. Commun. 2004, 320:1043-1050.
7. Jung M., et al. In search of suitable reference genes for gene expression studies of human renal cell carcinoma by real-time PCR. BMC Mol. Biol. 2007, 8:47.
8. Kostrominova T.Y., et al. Comparison of gene expression of 2-mo denervated, 2-mo stimulated-denervated, and control rat skeletal muscles. Physiol. Genomics 2005, 22:227-243.
9. Mahoney D.J., et al. Real-time RT-PCR analysis of housekeeping genes in human skeletal muscle following acute exercise. Physiol. Genomics 2004, 18:226-231.
10. Murphy R.M., et al. Effects of creatine supplementation on housekeeping genes in human skeletal muscle using real-time RT-PCR. Physiol. Genomics 2003, 12:163-174.
11. Ohl F., et al. Gene expression studies in prostate cancer tissue: which reference gene should be selected for normalization. J. Mol. Med. 2005, 83:1014-1024.
12. Ruijter J.M., et al. Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data. Nucleic Acids Res. 2009, 37:e45.
13. Touchberry C.D., et al. Age-related changes in relative expression of real-time PCR housekeeping genes in human skeletal muscle. J. Biomol. Tech. 2006, 17:157-162.
14. Vandesompele J., et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002, 3. RESEARCH0034.
15. Wittwer C.T., et al. High-resolution genotyping by amplicon melting analysis using LCGreen. Clin. Chem. 2003, 49:853-860.