Microbodies™

Methods in Molecular Biology

Volumn 535, Issue , 2009, Pages 361-372

  Schmoldt, Hans Ulrich ^a   Daneschdar, Matin ^b   Kolmar, Harald ^b   Blind, Michael ^a  

^a NascaCell Technologies AG   (Germany)

^b DARMSTADT UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

Barnase; Cystine knot protein; peptide agonist; pharmacophoric scaffold; thrombopoetin receptor

Indexed keywords

CYSTEINE; LIGAND; PEPTIDE; THROMBOPOIETIN RECEPTOR;

AMINO ACID SEQUENCE; ARTICLE; BIOMIMETICS; CELL LINE; CELL PROLIFERATION; CHEMISTRY; DRUG POTENTIATION; GENETICS; HUMAN; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; PLASMID; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN MULTIMERIZATION;

AMINO ACID SEQUENCE; BIOMIMETICS; CELL LINE; CELL PROLIFERATION; CYSTEINE; HUMANS; LIGANDS; MOLECULAR SEQUENCE DATA; PEPTIDES; PLASMIDS; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN MULTIMERIZATION; RECEPTORS, THROMBOPOIETIN;

EID: 77950625341     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-557-2_20     Document Type: Article

References (24)

1. Craik, D.J., Daly, N.L. and Waine, C. (2001) The cystine knot motif in toxins and implications for drug design. Toxicon 39, 1, 43-60.
2. Pallaghy, P.K., Nielsen, K.J., Craik, DJ. and Norton, R.S. (1994) A common structural motif incorporating a cystine knot and a triple-stranded beta-sheet in toxic and inhibitory polypeptides. Protein Sci. 3, 1833-1839. (Pubitemid 24356606)
3. Avrutina, O., Schmoldt, H.-U., Kolmar, H. and Diederichsen, U. (2004) Fmoc assisted synthesis of a 29-residue cystine-knot trypsin inhibitor containing a guaninyl amino acid at the P1 po-sition. Eur. J. Org. Chem. 23, 4931-4935. (Pubitemid 40006832)
4. Avrutina, O., Schmoldt, H.-U., Gabrijelcic-Geiger, D., Le Nguyen, D., Sommerhoff, C.P., Diederichsen, U. and Kolmar, H. (2005) Trypsin inhibition by macrocyclic and open chain variants of the squash inhibitor MCoTI-II. Biol. Chem. 386, 1301-1306. (Pubitemid 41794058)
5. Schmoldt, H.-U., Wentzel, A., Becker, S. and Kolmar, H. (2004) A fusion protein system for the recombinant production of short disulfide bond rich cystine-knot peptides using barnase as a purification handle. Protein Expr. Purif. 39, 82-89. (Pubitemid 39645385)
6. Krause, S., Schmoldt, H.-U., Wentzel, A., Ballmaier, M., Friedrich, K and Kolmar, H. (2007) Grafting of thrombopoietin-mimetic peptides into cystine knot miniproteins yields high-affinity thrombopoietin antagonists and agonists. FEBS J. 274, 86-95. (Pubitemid 44952900)
7. Reiss, S., Sieber, M., Oberle, V., Wentzel, A., Spangenberg, P, Claus, R., Kolmar, H. and Losche, W. (2006) Inhibition of platelet aggregation by grafting RGD and KGD sequences on the structural scaffold of small disulfide-rich proteins. Platelets 17, 3, 153-157. (Pubitemid 43791626)
8. Wentzel, A., Christmann, A., Kratzner, R and Kolmar, H. (1999) Sequence requirements of the GPNG/3-turn of the Ecballium elaterium trypsin inhibitor II explored by combinatorial library screening. J. Biol. Chem. 274, 21037-21043.
9. Wentzel, A., Christmann, A., Adams, T. and Kolmar, H. (2001) Display of passenger proteins on the surface of Escherichia coli K-12 by the enterohemorrhagic E. coli intimin EaeA. J. Bacteriol. 183, 7273-7284. (Pubitemid 33121862)
10. Baggio, R., Burgstaller, P., Hale, S.P., Putney, A.R., Lane, M., Lipovsek, D., Wright, M.C., Roberts, R.W., Liu, R., Szostak, J.W. and Wagner, R.W. (2002) Identification of epitope-like consensus motifs using mRNA display. J. Mol. Recognit. 15, 3, 126-134.
11. Kaushansky, K., Lok, S., Holly, R.D., Broudy, V.C., Lin, N., Bailey, M.C., For-strom, J.W., Buddle, M.M., Oort, P.J., Hagen, F.S. et al. (1994) Promotion of megakaryocyte progenitor expansion and differentiation by the c-Mpl ligand thrombo-poietin. Nature 369, 568-571. (Pubitemid 24192526)
12. de Sauvage, F.J., Hass, P.E., Spencer, S.D., Malloy, B.E., Gurney, A.L., Spencer, S.A., Darbonne, W.C., Henzel, W.J., Wong, S.C., Kuang, W.J. et al. (1994) Stimulation of megakaryocytopoiesis and thrombopoi-esis by the c-Mpl ligand. Nature 369, 533-538.
13. Methia, N., Louache, F., Vainchenker, W. and Wendling, F. (1993) Oligodeoxynu-cleotides antisense to the protooncogene c-mpl specifically inhibit in vitro megakaryocy-topoiesis. Blood 82, 1395-1401. (Pubitemid 23263953)
14. Heitz, A., Chiche, L., Le-Nguyen, D. and Castro, B. (1989) 1H, 2D NMR and distance ge-ometry study of the folding of Ecballium elaterium trypsin inhibitor, a member of the squash inhibitors family. Biochemistry 28, 2392-2398.
15. Jackson, P.J., McNulty, J.C., Yang, Y.K., Thompson, D.A., Chai, B., Gantz, I., Barsh, G.S. and Millhauser, G.L. (2002) Design, pharmacology, and NMR structure of a minimized cystine knot with agouti-related protein activity. Biochemistry 41, 7565-7572. (Pubitemid 34627783)
16. Cwirla, S.E., Balasubramanian, P., Duffin, D.J., Wagstrom, C.R., Gates, C.M., Singer, S.C., Davis, A.M., Tansik, R.L., Mattheakis, L.C., Boytos, C.M. et al. (1997) Peptide agonist of the thrombopoietin receptor as potent as the natural cytokine. Science 276, 1696-1699. (Pubitemid 27421976)
17. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning, A Labora-tory-Manual, Second ed. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
18. Schägger, H. and von Jagow, G. (1987) Tri-cine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis fort he separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166, 2, 368-379. (Pubitemid 18004907)
19. Gelly, J.C., Gracy, J., Kaas, Q., Le-Nguyen, D., Heitz, A. and Chiche, L. (2004) The KNOTTIN website and database: a new information system dedicated to the knottin scaffold. Nucleic Acids Res. 32, 1, 156-159.
20. Döbeli, H., Andres, H., Breyer, N., Draeger, N., Sizmann, D., Zuber, M.T., Wein-ert, B. and Wipf, B. (1998) Recombinant fusion proteins for the industrial production of dis-ulfide bridge containing peptides: purification, oxidation without concatamer formation, and selective cleavage. Protein Expr. Purif. 12, 404-414. (Pubitemid 28360435)
21. Fairlie, W.D., Uboldi, A.D., De Souza, D.P., Hemmings, G.J., Nicola, N.A. and Baca, M. (2002) A fusion protein system for the recombinant production of short disulfide-containing pep-tides. Protein Expr. Purif. 26, 171-178. (Pubitemid 35307501)
22. Chen, X.M., Qian, Y.W., Chi, C.W., Gan, K.D., Zhang, M.F. and Chen, C.Q. (1992) Chemical synthesis, molecular cloning, and expression of the gene coding for the Tricho-santhes trypsin inhibitor-a squash family inhibitor. J. Biochem. 112, 45-51.
23. Kojima, S., Miyoshi, K. and Miura, K. (1996) Synthesis of a squash-type protease inhibitor by gene engineering and effects of replacements of conserved hydrophobic amino acid residues on its inhibitory activity. Protein Eng. 9, 1241-1246.
24. Villa, S., De Fazio, G. and Canosi, U. (1989) Cyanogen bromide cleavage at methionine residues of polypeptides containing disulfide bonds. Anal. Biochem. 177, 161-164. (Pubitemid 19081739)