Large-scale Ficoll gradient separations using a commercially available, effectively closed, system

Cytotherapy

Volumn 12, Issue 3, 2010, Pages 418-424

  Janssen, William E ^a   Ribickas, Albert ^a   Meyer, Laura V ^a   Smilee, Renee C ^a  

^a H LEE MOFFITT CANCER CENTER AND RESEARCH INSTITUTE   (United States)

Author keywords

Clinical scale; Closed system; Ficoll; Gradient separation

Indexed keywords

FICOLL;

ARTICLE; BLOOD SALVAGE; BLOOD STORAGE; CELL COUNT; DENDRITIC CELL; ERYTHROCYTE; INSTRUMENT; MONOCYTE; MONONUCLEAR CELL; NEUTROPHIL; PRIORITY JOURNAL; SEPARATION TECHNIQUE;

EID: 77950585305     PISSN: 14653249     EISSN: 14772566     Source Type: Journal    
DOI: 10.3109/14653240903479663     Document Type: Article

References (9)

1. Antonia SJ, Mirza N, Fricke I, Chiappori A, Thompson P, Williams N, et al. Combination of p53 cancer vaccine with chemotherapy in patients with extensive stage small cell lung cancer. Clin Cancer Res. 2006;12:878-887 (Pubitemid 43259871)
2. Dohnal AM, Graffi S, Witt V, Eichstill C, Wagner D, Ul-Haq S, et al. Comparative evaluation of techniques for the manufacturing of dendritic cell-based cancer vaccines. J Cell Mol Med. 2009;13:125-135
3. Elkord E, Williams PE, Kynaston H, Rowbottom AW. Human monocyte isolation methods infl uence cytokine production from in vitro generated dendritic cells. Immunology. 2005; 114:204-212 (Pubitemid 40208937)
4. Felzmann T, Witt V, Wimmer D, Ressmann G, Wagner D, Paul P, et al. Monocyte enrichment from leukapharesis products for the generation of DCs by plastic adherence, or by positive or negative selection. Cytotherapy. 2003;5: 391-398 (Pubitemid 37369026)
5. Meyer-Wentrup F, Burdach S. Effi cacy of dendritic cell generation for clinical use: recovery and purity of monocytes and mature dendritic cells after immunomagnetic sorting or adherence selection of CD14 starting populations. J Hematother Stem Cell Res. 2003;12:289-299 (Pubitemid 36736740)
6. Noble PB, Cutts JH, Carroll KK. Ficoll fl otation for the separation of blood leukocyte types. Blood. 1968;31:66-73.
7. Aktas M, Radke TF, Strauer BE, Wernet P, Kogler G. Separation of adult bone marrow mononuclear cells using the automated closed separation system Sepax. Cytotherapy. 2008;10:203-211
8. Janssen WE, Lee C, Smilee R, Carter R. Use of the Terumo SteriCell for the processing of bone marrow and peripheral blood stem cells. J Hematother. 1992;1:349-359
9. Abdelkefi A, Maamar M, Torjman L, Ladeb S, Lakhal A, Othman TB, et al. Prospective randomised comparison of the COBE Spectra version 6 and Haemonetics MCS- cell separators for hematopoietic progenitor cell leucapheresis in patients with multiple myeloma. J Clin Apher. 2006; 21:111-115