Troubleshooting immunohistochemical labelling of proliferating cell nuclear antigen (PCNA) in cryocut tissue sections of mouse prostate

Journal of Pharmacological and Toxicological Methods

Volumn 61, Issue 2, 2010, Pages 98-101

  Xie, Jin Han ^a   Hollande, Frédéric ^b,c   Haynes, John M ^a   Pouton, Colin W ^a   Ventura, Sabatino ^a  

^a MONASH UNIVERSITY   (Australia)

^b INSTITUT DE GÉNOMIQUE FONCTIONNELLE   (France)

^c UNIV MONTPELLIER   (France)

Author keywords

Fixation; Frozen sections; Immunohistochemical methods; Incubation temperature; Mouse; Permeabilization

Indexed keywords

CYCLINE; LIQUID NITROGEN; PARAFORMALDEHYDE; PROTEIN ANTIBODY; TRITON X 100;

ANIMAL TISSUE; ANTIBODY LABELING; ARTICLE; CELL CYCLE S PHASE; CONCENTRATION RESPONSE; CONTROLLED STUDY; FROZEN SECTION; IMMUNOFLUORESCENCE TEST; IMMUNOHISTOCHEMISTRY; INCUBATION TEMPERATURE; INCUBATION TIME; MALE; MITOSIS; MOUSE; NONHUMAN; PERMEABILITY; PROSTATE; PROTEIN ANALYSIS; ROOM TEMPERATURE; STROMA; TISSUE FIXATION; TISSUE SECTION;

MUS;

EID: 77950531865     PISSN: 10568719     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vascn.2009.11.001     Document Type: Article

References (10)

1. Almendral J.M., Huebsch D., Blundell P.A., Macdonald-Bravo H., Bravo R. Cloning and sequence of the human nuclear protein cyclin: homology with DNA-binding proteins. Proceedings of the National Academy of Sciences of the United States of America 1987, 84:1575-1579.
2. Baum H.P., Reichrath J., Theobald A., Schock G. Fixation requirements for the immunohistochemical reactivity of PCNA antibody PC10 on cryostat sections. Histochemical Journal 1994, 26:929-933.
3. Bravo R., Macdonald-Bravo H. Existance of two populations of cyclin / proliferating cell nuclear antigen during the cell cycle: association with DNA replication sites. Journal of Cell Biology 1987, 105:1549-1554.
4. Celis J.E., Celis A. Cell cycle-dependent variations in the distribution of the nuclear protein cyclin proliferating cell nuclear antigen in cultured cells: subdivision of S phase. Proceedings of the National Academy of Sciences of the United States of America 1985, 82:3262-3266.
5. Hall P.A., Levison D.A., Woods A.L., Yu C.C., Kellock D.B., Watkins J.A., Barnes D.M., Gillett C.E., Camplejohn R., Dover R., Waseem N.H., Lane D.P. Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms. Journal of Pathology 1990, 162:285-294.
6. Landberg G., Roos G. Antibodies to proliferating cell nuclear antigen as S-phase probes in flow cytometric cell cycle analysis. Cancer Research 1991, 51:4570-4574.
7. Roos G., Landberg G., Huff J.P., Houghten R., Takasaki Y., Tan E.M. Analysis of the epitopes of proliferating cell nuclear antigen recognized by monoclonal antibodies. Laboratory Investigation 1993, 68:204-210.
8. Sasaki K., Kurose A., Ishida Y. Flow cytometric analysis of the expression of PCNA during the cell cycle in HeLa cells and effects of the inhibition of DNA synthesis on it. Cytometry 1993, 14:876-882.
9. Tsuji T., Shrestha P., Yamada K., Takagi H., Shinozaki F., Sasaki K., Maeda K., Mori M. Proliferating cell nuclear antigen in malignant and pre-malignant lesions of epithelial origin in the oral cavity and the skin: an immunohistochemical study. Virchows Archive A: Pathoogical Anatomy and Histopathology 1992, 420:377-383.
10. Zhong W., Peng J., He H., Wu D., Han Z., Bi X., Dai Q. Ki-67 and PCNA expression in prostate cancer and benign prostatic hyperplasia. Clinical and Investigative Medicine 2008, 31:E8-E15.