Prevalence of salmonella typhi among patients with febrile illness in rural and peri-urban populations of vellore district, as determined by nested PCR targeting the flagellin gene

Molecular Diagnosis and Therapy

Volumn 14, Issue 2, 2010, Pages 107-112

  Nandagopal, Balaji ^a   Sankar, Sathish ^a   Lingesan, Karthikeyan ^a   Appu, Kumarasekharan Chandrasekharan ^a   Padmini, Baby ^b   Sridharan, Gopalan ^a   Gopinath, Anil Kumar ^c  

^a Sri Narayani Hospital   (India)

^b G KUPPUSWAMY NAIDU MEMORIAL HOSPITAL   (India)

^c VIT UNIVERSITY   (India)

Author keywords

Diagnosis; Diagnostic tests; Diagnostics; Fever; Salmonella infections; Typhoid

Indexed keywords

FLAGELLIN;

ADOLESCENT; ADULT; AGED; ARTICLE; BLOOD CULTURE; CHILD; FEMALE; HUMAN; INDIA; MAJOR CLINICAL STUDY; MALE; POLYMERASE CHAIN REACTION; PRESCHOOL CHILD; PREVALENCE; PRIORITY JOURNAL; RURAL POPULATION; SALMONELLA TYPHI; SCHOOL CHILD; SENSITIVITY AND SPECIFICITY; TYPHOID FEVER; URBAN POPULATION;

SALMONELLA; SALMONELLA TYPHI;

EID: 77950523645     PISSN: 11771062     EISSN: 11792000     Source Type: Journal    
DOI: 10.1007/bf03256360     Document Type: Article

References (22)

1. Jung A, Singh MM, Jajoo U. Unexplained fever-analysis of 233 cases in a referral hospital. Indian J Med Sci 1999; 53: 535-544
2. Kejariwal D, Sarkar N, Chakraborti SK, et al. Pyrexia of unknown origin: a prospective study of 100 cases. J Postgrad Med 2001; 47: 104-107
3. WHO Initiative for Vaccine Research. Diarrhoeal diseases: typhoid fever. Geneva: WHO, 2009 Feb [online]. Available from URL: http://www.who.int/ vaccine-research/diseases/diarrhoeal/en/index7.html#bacteriology [Accessed 2010 Jan 21]
4. Arjyal A, Pandit A. Treatment of enteric fever. J Infect Developing Countries 2008; 2 (6): 426-430
5. Itah AY, Uweh EE. Bacteria isolated from blood, stool and urine of typhoid patients in a developing country. Southeast Asian J TropMed Public Health 2005; 36 (3): 673-677
6. Bhutta ZA, Mansurali N. Rapid serologic diagnosis of pediatric typhoid fever in an endemic area: a prospective comparative evaluation of two dot-enzyme immunoassays and the Widal test. Am J Trop Med Hyg 1999; 61 (4): 654-657
7. Hatta M, Goris MGA, Heerkens E, et al. Simple dipstick assay for the detection of Salmonella typhi-specific IgM antibodies and the evolution of the immune response in patients with typhoid fever. Am J Trop Med Hyg 2002; 66 (4): 416-421
8. Pastoor R, Hatta M, Abdoel TH, et al. Simple, rapid, and affordable point-ofcare test for the serodiagnosis of typhoid fever. Diagn Microbiol Infect Dis 2008; 61 (2): 129-134
9. Kumar S, Balakrishna K, Batra HV. Enrichment-ELISA for detection of Salmonella typhi from food and water samples. Biomed Environ Sci 2008; 21 (2): 137-143
10. Santiviago CA, Toro CS, Bucarey SA, et al. A chromosomal region surrounding the ompD porin gene marks a genetic difference between Salmonella typhi and the majority of Salmonella serovars. Microbiol 2001; 147: 1897-1907 (Pubitemid 32684931)
11. Hirose K, Itoh KI, Nakajima H, et al. Selective amplification of tyv (rfbE), prt (rfbS), viaB, and fliC genes by multiplex PCR for identification of Salmonella enterica serovars typhi and paratyphi A. J Clin Microbiol 2002; 40 (2): 633-636
12. Levy H, Diallo S, Tennant SM, et al. PCR method to identify Salmonella enterica serovars typhi, paratyphi A, and paratyphi B among salmonella isolates from the blood of patients with clinical enteric fever. J Clin Microbiol 2008; 46 (5): 1861-1866 (Pubitemid 351657245)
13. Wain J, Hosoglu S. The laboratory diagnosis of enteric fever. J Infect Developing Countries 2008; 2 (6): 421-425
14. Song JH, Cho H, Park MY, et al. Detection of Salmonella typhi in the blood of patients with typhoid fever by polymerase chain reaction. J Clin Microbiol 1993; 31 (6): 1439-1443
15. Kermekchiev MB, Tzekov A, Barnes WM. Cold-sensitive mutants of Taq DNA polymerase provide a hot start for PCR. Nucleic Acids Res 2003; 3: 6139-6147 (Pubitemid 37442341)
16. Wain J, Diep TS, Ho VA, et al. Quantitation of bacteria in blood of typhoid fever patients and relationship between counts and clinical features, transmissibility, and antibiotic resistance. J Clin Microbiol 1998; 36 (6): 1683-1687 (Pubitemid 28226053)
17. Hashimoto Y, Itho Y, Fujinaga Y, et al. Development of nested PCR based on the ViaB sequence to detect Salmonella typhi. J Clin Microbiol 1995; 33 (3): 775-777
18. Naravaneni R, Jamil K. Rapid detection of food-borne pathogens by using molecular techniques. J Med Microbiol 2005; 54: 51-54
19. Prakash P, Mishra OP, Singh AK, et al. Evaluation of nested PCR in diagnosis of typhoid fever. J Clin Microbiol 2005; 43 (1): 431-432 (Pubitemid 40096335)
20. Hatta M, Smits HL. Detection of Salmonella typhi by nested polymerase chain reaction in blood, urine and stool samples. Am J Trop Med Hyg 2007; 76 (1): 139-143
21. Shafazand S, Weinacker AB. Blood cultures in the critical care unit. Chest 2002; 122: 1727-1736
22. Connell TG, Rele M, Cowley D, et al. How reliable is a negative blood culture result? Volume of blood submitted for culture in routine practice in a children's hospital. Pediatrics 2007; 119: 891-896 (Pubitemid 46715658)