Structural and functional characterization of H+,K+-ATPase with bound fluorinated phosphate analogs

Journal of Structural Biology

Volumn 170, Issue 1, 2010, Pages 60-68

  Abe, Kazuhiro ^a   Tani, Kazutoshi ^a   Fujiyoshi, Yoshinori ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

Cryo electron microscopy; H+,K+ ATPase; Membrane protein structure; P type ATPase; Two dimensional crystal

Indexed keywords

HYDROGEN POTASSIUM ADENOSINE TRIPHOSPHATASE; MAGNESIUM ION; POTASSIUM ION; SARCOPLASMIC RETICULUM CALCIUM TRANSPORTING ADENOSINE TRIPHOSPHATASE;

ARTICLE; BINDING AFFINITY; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME REACTIVATION; ENZYME STABILITY; ENZYME STRUCTURE; IC 50; PH; PRIORITY JOURNAL; STRUCTURE ANALYSIS; SURFACE PROPERTY; TEMPERATURE;

CRYOELECTRON MICROSCOPY; CRYSTALLIZATION; FLUORIDES; H(+)-K(+)-EXCHANGING ATPASE; MODELS, MOLECULAR; PHOSPHATES; PHOSPHORYLATION; PROTEIN CONFORMATION;

EID: 77950518814     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2009.12.008     Document Type: Article

References (34)

1. +-ATPase prevents reverse reaction of the transport cycle. EMBO J. 2009, 28:1637-1643.
2. Agard D.A. A least-squares method for determining structure factors in three-dimensional tilted-view reconstructions. J. Mol. Biol. 1983, 167:849-852.
3. Bigay J., Deterre P., Pfister C., Chabre M. Fluoride complexes of aluminium or beryllium act on G-proteins as reversibly bound analogues of the gamma phosphate of GTP. EMBO J. 1987, 6:2907-2913.
4. Chifflet S., Torriglia A., Chiesa R., Tolosa S. A method for the determination of inorganic phosphate in the presence of labile organic phosphate and high concentrations of protein: application to lens ATPases. Anal. Biochem. 1988, 168:1-4.
5. Chow D.C., Forte J.G. Functional significance of the beta-subunit for heterodimeric P-type ATPases. J. Exp. Biol. 1995, 198:1-17.
6. Crowther R.A., Henderson R., Smith J.M. MRC image processing programs. J. Struct. Biol. 1996, 116:9-16.
7. 2+-ATPase: changes in catalytic and transport sites during phosphoenzyme hydrolysis. J. Biol. Chem. 2004, 279:14991-14998.
8. 2+ by beryllium fluoride: structural changes during phosphorylation and isomerization. J. Biol. Chem. 2009, 284:22722-22735.
9. 2 state stabilization by specific alpha/beta-subunit interactions of Na,K- and H,K-ATPase. J. Biol. Chem. 2008, 284:3842-3854.
10. Dürr K.L., Abe K., Tavraz N.N., Friedrich T. E2P-state stabilization by the N-terminal tail of the H,K-ATPase β-subunit is critical for efficient proton pumping under in vivo conditions. J. Biol. Chem. 2009, 284:20147-20154.
11. Fujiyoshi Y. The structural study of membrane proteins by electron crystallography. Adv. Biophys. 1998, 35:25-80.
12. Fujiyoshi Y., Mizusaki T., Morikawa K., Yamagishi H., Aoki Y., Kihara H., Harada Y. Development of a superfluid helium stage for high-resolution electron microscopy. Ultramicroscopy 1991, 38:241-251.
13. +-stimulated ATPase in purified microsomes of bullfrog oxynic cells. Biochim. Biophys. Acta 1973, 307:169-180.
14. Gyobu N., Tani K., Hiroaki Y., Kamegawa A., Mitsuoka K., Fujiyoshi Y. Improved specimen preparation for cryo-electron microscopy using a symmetric carbon sandwich technique. J. Struct. Biol. 2004, 146:325-333.
15. +)-ATPase. Biochim. Biophys. Acta 1985, 821:377-383.
16. Henderson R., Baldwin J., Downing K., Lepault J., Zemiln F. Structure of purple membrane from Halobacterium halobium: recording, measurement and evaluation of electron micrographs at 3.5 Å resolution. Ultramicroscopy 1986, 19:147-178.
17. 2+-ATPase. Biochim. Biophys. Acta 2007, 1767:1310-1318.
18. Lutsenko S., Kaplan J.H. An essential role for the extracellular domain of the sodium-potassium-ATPase β-subunit in cation occlusion. Biochemistry 1993, 32:6737-6743.
19. Morth J.P., Poulsen H., Toustrup-Jensen M.S., Schack V.R., Egebjerg J., Andersen J.P., Vilsen B., Nissen P. Crystal structure of the sodium-potassium pump. Nature 2007, 450:1043-1049.
20. +-ATPase in different states of the transport cycle. J. Struct. Biol. 2008, 162:219-228.
21. Olesen C., Picard M., Winther A.M., Gyrup C., Morth J.P., Oxvig C., Møller J.V., Nissen P. The structural basis of calcium transport by the calcium pump. Nature 2007, 450:1036-1042.
22. Palmgren M.G., Axelsen K.B. Evolution of P-type ATPases. Biochim. Biophys. Acta 1998, 1365:37-45.
23. Petsko G.A. Chemistry and biology. Proc. Natl. Acad. Sci. USA 2000, 97:538-590.
24. Post R.L., Kume S. Evidence for an aspartyl phosphate residue at the active site of sodium and potassium ion transport adenosine triphosphatase. J. Biol. Chem. 1973, 248:6993-7000.
25. Rabon E.C., Reuben M.A. The mechanism and structure of the gastric H,K-ATPase. Annu. Rev. Physiol. 1990, 52:321-344.
26. + pump in plasma membrane of hog stomach. J. Biol. Chem. 1976, 251:7690-7698.
27. Sachs G., Shin J.M., Vagin O., Lambrecht N., Yakubov I., Munson K. The gastric H,K ATPase as a drug target past, present, and future. J. Clin. Gastroenterol. 2007, 41:S226-S242.
28. Shinoda T., Ogawa H., Cornelius F., Toyoshima C. Crystal structure of the sodium-potassium pump at 2.4 Å resolution. Nature 2009, 459:446-450.
29. Tani K., Sasabe H., Toyoshima C. A set of computer programs for determining defocus and astigmatism in electron images. Ultramicroscopy 1996, 65:31-44.
30. Toyoshima C., Nomura H., Tsuda T. Luminal gating mechanism revealed in calcium pump crystal structures with phosphate analogues. Nature 2004, 432:361-368.
31. Toyoshima C., Norimatsu Y., Iwasawa S., Tsuda T., Ogawa H. How processing of aspartylphosphate is coupled to luminal gating of the ion pathway in the calcium pump. Proc. Nat. Acad. Sci. USA 2007, 104:19831-19836.
32. Volkmann N.A. Novel three-dimensional variant of the watershed transform for segmentation of electron density maps. J. Struct. Biol. 2002, 138:123-129.
33. +-ATPase-rich vesicles: implications for HCl secretion and parietal cell physiology. Am. J. Physiol. 1985, 248:G595-G607.
34. Yen L.A., Cosgrove P., Holt W. SDS purification of porcine H,K-ATPase from gastric mucosa. Membr. Biochem. 1990, 9:129-140.