Real-time PCR method for the quantification of Burkholderia cepacia complex attached to lung epithelial cells and inhibition of that attachment

Letters in Applied Microbiology

Volumn 50, Issue 5, 2010, Pages 500-506

  Wright, C ^a   Herbert, G ^a   Pilkington, R ^a   Callaghan, M ^a   McClean, S ^a  

^a DUBLIN INSTITUTE OF TECHNOLOGY   (Ireland)

Author keywords

Bacterial adhesion; Burkholderia cepacia complex; Cystic fibrosis; Lung epithelial cells; Real time PCR

Indexed keywords

ADHESION; AMPLIFICATION; BIOLOGICAL ORGANS; CELLS; GLUCOSE; MAMMALS; SUGAR (SUCROSE); SUGAR SUBSTITUTES;

BACTERIAL ADHESION; BURKHOLDERIA CEPACIA COMPLEX; CYSTIC FIBROSIS; LUNG EPITHELIAL CELLS; REAL-TIME PCR;

BACTERIOLOGY;

ADHESIN; BACTERIAL DNA; GALACTOSE; GLUCOSE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; LACTOSE; MANNOSE; RECA PROTEIN; SUGAR; XYLITOL; CARBOHYDRATE;

AMPLIFICATION; BACTERIUM; GLUCOSE; INCUBATION; INHIBITION; PATHOGEN; POLYMERASE CHAIN REACTION; REAL TIME;

ARTICLE; BACTERIAL COUNT; BACTERIUM ADHERENCE; BINDING AFFINITY; BURKHOLDERIA; BURKHOLDERIA CEPACIA COMPLEX; BURKHOLDERIA MULTIVORANS; CELL ADHESION; COLONY FORMING UNIT; CONTROLLED STUDY; CYSTIC FIBROSIS; GENE AMPLIFICATION; HUMAN; HUMAN CELL; LUNG ALVEOLUS CELL; NONHUMAN; PLATING MEDIUM; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; BURKHOLDERIA INFECTION; CELL LINE; CYTOLOGY; DRUG EFFECTS; EPITHELIUM CELL; EVALUATION STUDY; GENETICS; ISOLATION AND PURIFICATION; LUNG; MICROBIOLOGY; PHYSIOLOGY; POLYMERASE CHAIN REACTION; PROCEDURES;

BACTERIA (MICROORGANISMS); BURKHOLDERIA CEPACIA COMPLEX; BURKHOLDERIA MULTIVORANS; MAMMALIA;

BACTERIAL ADHESION; BURKHOLDERIA CEPACIA COMPLEX; BURKHOLDERIA INFECTIONS; CARBOHYDRATES; CELL LINE; EPITHELIAL CELLS; HUMANS; LUNG; POLYMERASE CHAIN REACTION;

EID: 77950468263     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2010.02828.x     Document Type: Article

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