Direct determination of tissue aminothiol, disulfide, and thioether levels using HPLC-ECD with a novel stable boron-doped diamond working electrode

Methods in Molecular Biology

Volumn 594, Issue , 2010, Pages 327-339

  Bailey, Bruce ^a   Waraska, John ^a   Acworth, Ian ^a  

^a NONE

Author keywords

Boron doped diamond; Brain; Cysteine; Cystine; Disulfide; Electrochemical detection; Glutathione; Glutathione disulfide; HPLC; Liver; Plasma; S nitrosoglutathione; Thiol

Indexed keywords

BORON; CYSTEINE; DIAMOND; DISULFIDE; GLUTATHIONE; HOMOCYSTEINE; SULFIDE;

ANIMAL; ARTICLE; CHEMISTRY; ELECTROCHEMISTRY; ELECTRODE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; METHODOLOGY; REPRODUCIBILITY;

ANIMALS; BORON; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYSTEINE; DIAMOND; DISULFIDES; ELECTROCHEMISTRY; ELECTRODES; GLUTATHIONE; HOMOCYSTEINE; HUMANS; REPRODUCIBILITY OF RESULTS; SULFIDES;

EID: 77950360610     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60761-411-1_23     Document Type: Article

References (13)

1. Acworth IN (2003) The handbook of redox biochemistry. CD-ROM. ESA Biosciences. Part number: 70-6090
2. Fariss MW, Reed DJ (1987) High-performance liquid chromatography of thiols and disul-fdes: Dinitrophenol derivatives. Methods Enzymol 143:101-109
3. Keller DA, Menzel DB (1985) Picomole analysis of glutathione, glutathione disulfde, glu-tathione S-sulfonate, and cysteine S-sulfonate by high-performance liquid chromatography. Anal Biochem 151:418-423
4. Michelet F, Gueguen R, Leroy P, Wellman M, Nicolas A, Siest G (1995) Blood and plasma glutathione measured in healthy subjects by HPLC: Relation to sex, aging, biological variables, and life habits. Clin Chem 41: 1509-1517
5. Fahey RC, Newton GL (1987) Determination of low-molecular-weight thiols using mono-bromobimane fuorescent labeling and high-performance liquid chromatography. Methods Enzymol 143:85-96
6. Martin J, White IN (1991) Fluorimetric determination of oxidised and reduced glutathione in cells and tissues by high-performance liquid chromatography following derivati-zation with dansyl chloride. J Chromatogr 568:219-225
7. Acworth IN, Bowers M (1997) An introduction to HPLC-based electrochemical detection: From single electrode to multi-electrode arrays. In: Acworth IN, Naoi M, Parvez H, Parvez S (eds) Coulometric electrode array detectors for HPLC. Progress in HPLC-HPCE, vol 6. VSP, Utrecht, The Netherlands, pp 3-50
8. Flanagan RJ, Perrett D, Whelpton R (eds) (2005) Thiols, disulfdes and related compounds. In: Smith RM (Series ed) Electrochemical detection in HPLC: Analysis of drugs and poisons. RSC chromatography monographs, Athenaeum, UK, pp79-103
9. Xu J, Granger M, Chen Q, Strojek JW, Lister TE, Swain GM (1997) Boron-Doped Diamond Thin-Film Electrodes: Diamond thin flms could be an electrochemist's best friend. Anal Chem 69:591A-597A
10. Rao T, Fujishima A, Angus J (2005) In: Fujishima A, Einaga Y, Rao T, Tryk D (eds) Historical survey of diamond electrodes in diamond electrochemistry. Elsevier, Amsterdam, pp 1-10
11. Waraska J, Acworth IN (2007) A novel electrode material that extends the utility of HPLC-electrochemical detection. American Lab 39(14): 38-41
12. Jones DP, Carlson JL, Samiec PS, Sternberg P, Mody VC, Reed RL, Brown LA (1998) Glutathione measurement in human plasma. Evaluation of sample collection, storage and derivatization conditions for analysis of derivatives by HPLC. Clin Chim Acta 275: 175-184
13. Zhang Y, Hogg N (2005) S-Nitrosothiols: Cellular formation and transport. Free Radic Biol Med 38: 831-838