Loss of heterozygosity in Phytophthora capsici after N-ethyl-nitrosourea mutagenesis

Mycologia

Volumn 102, Issue 1, 2010, Pages 27-32

  Hulvey, Jon ^a   Young, Jacque ^a   Finley, Ledare ^a   Lamour, Kurt ^a  

^a UNIVERSITY OF TENNESSEE   (United States)

Author keywords

Loss of heterozygosity (LOH); Mitotic recombination; Mutagenesis; N ethyl nitrosourea (ENU); Phytophthora capsici

Indexed keywords

FUNGUS; GENETIC VARIATION; GENOMICS; HETEROZYGOSITY; MUTATION; PATHOGENICITY; RECOMBINATION;

PHYTOPHTHORA; PHYTOPHTHORA CAPSICI;

ALKYLATING AGENT; ETHYLNITROSOUREA; MUTAGENIC AGENT;

ARTICLE; DRUG EFFECT; GENETIC RECOMBINATION; GENETICS; HETEROZYGOSITY LOSS; METHODOLOGY; MUTAGENESIS; PHYTOPHTHORA; POINT MUTATION; POLYMERASE CHAIN REACTION; TRANSITION TEMPERATURE;

ALKYLATING AGENTS; ETHYLNITROSOUREA; LOSS OF HETEROZYGOSITY; MUTAGENESIS; MUTAGENS; PHYTOPHTHORA; POINT MUTATION; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; TRANSITION TEMPERATURE;

EID: 77950351542     PISSN: 00275514     EISSN: 15572536     Source Type: Journal    
DOI: 10.3852/09-102     Document Type: Article

References (21)

1. Andersen MP, Nelson ZW, Hetrick ED, Gottschling DE. 2008. A genetic screen for increased loss of heterozygosity in Saccharomyces cerevisiae. Genetics 179(3):1179-1195.
2. Carter DA, Buck KW, Archer SA, van der Lee T, Shattock RC, Shaw DS. 1999. The detection of nonhybrid, trisomic, and triploid offspring in sexual progeny of a mating of Phytophthora infestans. Fungal Genet Biol 26:198-208.
3. Castro J, Zentmyer GA, Belser WL Jr. 1971. Induction of auxotrophic mutants in Phytophthora by UV light. Phytopathology 61:283-289.
4. Chamnanpunt J, Shan W, Tyler BM. 2001. High frequency mitotic gene conversion in genetic hybrids of the oomycete Phytophthora sojae. Proc Natl Acad Sci USA 98:14530-14535.
5. +/-3.7.2C mouse lymphoma cells. Mutagenesis 17:105-109.
6. Dobrowolski MP, Tommerup IC, Blakeman HD, O'Brien PA. 2002. Non-mendelian inheritance revealed in a genetic analysis of sexual progeny of Phytophthora cinnamomi with microsatellite markers. Fungal Genet Biol 35:197-212.
7. Förster H, Coffey MD. 1990. Mating behavior of Phytophthora parasitica evidence for sexual recombination in oospores using DNA restriction fragment length polymorphisms as genetic markers. Exp Mycol 14:351-359.
8. Francis DM, Gehlen MF, St Clair DA. 1994. Genetic variation in homothallic and hyphal swelling isolates of Pythium ultimum var. ultimum and P. ultimum var. sporangiferum. Mol Plant-Microbe Interact 7:766-775.
9. Freeman KM, Hoffmann GR. 2007. Frequencies of mutagen-induced coincident mitotic recombination at unlinked loci in Saccharomyces cerevisiae. Mutat Res 616:119-132.
10. Helleday T. 2003. Pathways of mitotic homologous recombination in mammals. Mutat Res 532:103-115.
11. Hurtado-Gonzales OP, Lamour KH. 2009. Evidence for inbreeding and apomixis in close crosses of Phytophthora capsici. Plant Pathol (In press).
12. Jagtar SD, Shabeg SS. 1992. Application of a wheat seedling assay for detecting aneuploidy induced by N-ethyl-N-nitrosourea and 4-nitroquinoline-l-oxide. Mutat Res 270:79-85.
13. Lamour KH, Finley L, Hurtado-Gonzales O, Gobena D, Tierney M, Meijer HJG. 2006. Targeted gene mutation in Phytophthora spp. Mol Plant-Microbe Interact 19: 1359-1367.
14. Lamour KH, Finley SL. 2006. A strategy for recovering high quality genomic DNA from a large number of Phytophthora isolates. Mycologia 98:514-517.
15. Meijer HJG, Latijnhouwers M, Ligterink W. 2005. A transmembrane phospholipase D in Phytophthora; a novel PLD subfamily. Gene (Amsterdam) 350:173-182.
16. Moore JL, Rush LM, Breneman C, Mohideen MPK, Cheng KC. 2006. Zebrafish genomic instability mutants and cancer susceptibility. Genetics 174:585-600.
17. Petes TD, Malone RE, Symington LS. 1991. The molecular and cellular biology of the yeast Saccharomyces: genome dynamics, protein synthesis, and energetics. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory.
18. Rozen R, Skaletsky HJ. 2000. Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S, eds. Bioinformatics methods and protocols: methods in molecular biology. Totowa, NJ: Humana Press. p 365-386.
19. Shinji H, Mayumi O, Kazuo A, Hisashi H, Yoshinori N, Rinji A. 2005. Isolation of auxotrophic mutants of diploid industrial yeast strains after UV mutagenesis. Appl Environ Microbiol 71:312-319.
20. Tsang PWK, Cao B, Siu PYL, Wang J. 1999. Loss of heterozygosity, by mitotic gene conversion and crossing over, causes strain-specific adenine mutants in constitutive diploid Candida albicans. Microbiology 145: 1623-1629.
21. Wu W, Pujol C, Lockhart SR, Soll DR. 2005. Chromosome loss followed by duplication is the major mechanism of spontaneous mating-type locus homozygosis in Candida albicans. Genetics 169:1311-1327.