Tigecycline resistance in Serratia marcescens associated with up-regulation of the SdeXY-HasF efflux system also active against ciprofloxacin and cefpirome

Journal of Antimicrobial Chemotherapy

Volumn 65, Issue 3, 2010, Pages 479-482

  Hornsey, Michael ^a   Ellington, Matthew J ^a,c   Doumith, Michel ^a   Hudson, Sue ^b   Livermore, David M ^a   Woodford, Neil ^a  

^a PUBLIC HEALTH ENGLAND   (United Kingdom)

^b QUEEN ELIZABETH HOSPITAL   (United Kingdom)

^c ADDENBROOKE S HOSPITAL   (United Kingdom)

Author keywords

Enterobacteriaceae; Glycylcycline; Quinolones; RND pumps

Indexed keywords

CARRIER PROTEIN; CEFPIROME; CIPROFLOXACIN; OUTER MEMBRANE PROTEIN; PROTEIN HASF; PROTEIN SDEXY; TIGECYCLINE; UNCLASSIFIED DRUG;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL STRAIN; CONTROLLED STUDY; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENE INACTIVATION; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; REGULATORY MECHANISM; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SERRATIA MARCESCENS; UPREGULATION;

ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; BIOLOGICAL TRANSPORT, ACTIVE; CEPHALOSPORINS; CIPROFLOXACIN; DRUG RESISTANCE, BACTERIAL; GENE DELETION; HUMANS; MEMBRANE TRANSPORT PROTEINS; MICROBIAL SENSITIVITY TESTS; MINOCYCLINE; MUTATION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SELECTION, GENETIC; SERRATIA INFECTIONS; SERRATIA MARCESCENS; UP-REGULATION;

EID: 77950320324     PISSN: 03057453     EISSN: 14602091     Source Type: Journal    
DOI: 10.1093/jac/dkp475     Document Type: Article

References (15)

1. Hejazi A, Falkiner FR. Serratia marcescens. J Med Microbiol 1997; 46: 903-12.
2. Reinert RR, Low DE, Rossi F et al. Antimicrobial susceptibility among organisms from the Asia/Pacific Rim, Europe and Latin and North America collected as part of TEST and the in vitro activity of tigecycline. J Antimicrob Chemother 2007; 60: 1018-29.
3. Keeney D, Ruzin A, McAleese F et al. MarA-mediated overexpression of the AcrAB efflux pump results in decreased susceptibility to tigecycline in Escherichia coli. J Antimicrob Chemother 2008; 61: 46-53.
4. Ruzin A, Visalli MA, Keeney D et al. Influence of transcriptional activator RamA on expression of multidrug efflux pump AcrAB and tigecycline susceptibility in Klebsiella pneumoniae. Antimicrob Agents Chemother 2005; 49: 1017-22.
5. Kumar A, Worobec EA. Cloning, sequencing, and characterization of the SdeAB multidrug efflux pump of Serratia marcescens. Antimicrob Agents Chemother 2005; 49: 1495-501.
6. Begic S, Worobec EA. Characterization of the Serratia marcescens SdeCDE multidrug efflux pump studied via gene knockout mutagenesis. Can J Microbiol 2008; 54: 411-6.
7. Chen J, Kuroda T, Huda MN et al. An RND-type multidrug efflux pump SdeXY from Serratia marcescens. J Antimicrob Chemother 2003; 52: 176-9.
8. Kumar A, Worobec EA. HasF, a TolC-homolog of Serratia marcescens,is involved in energy-dependent efflux. Can J Microbiol 2005; 51: 497-500.
9. Andrews JM. BSAC standardized disc susceptibility testing method (version 8). J Antimicrob Chemother 2009; 64: 454-89.
10. Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 1989.
11. -ΔΔCT method. Methods 2001; 25: 402-8.
12. Miller VL, Mekalanos JJ. A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J Bacteriol 1988; 170: 2575-83.
13. Peleg AY, Adams J, Paterson DL. Tigecycline efflux as a mechanism for nonsusceptibility in Acinetobacter baumannii. Antimicrob Agents Chemother 2007; 51: 2065-9.
14. Ruzin A, Keeney D, Bradford PA. AdeABC multidrug efflux pump is associated with decreased susceptibility to tigecycline in Acinetobacter calcoaceticus-Acinetobacter baumannii complex. J Antimicrob Chemother 2007; 59:1001-4.
15. Dean CR, Visalli MA, Projan SJ et al. Efflux-mediated resistance to tigecycline (GAR-936) in Pseudomonas aeruginosa PAO1. Antimicrob Agents Chemother 2003; 47: 972-8.