Mechanical and cell viability properties of crosslinked low- And high-molecular weight poly(ethylene glycol) diacrylate blends

Journal of Biomedical Materials Research - Part A

Volumn 93, Issue 2, 2010, Pages 558-566

  Mazzoccoli, Jason P ^a   Feke, Donald L ^a   Baskaran, Harihara ^a   Pintauro, Peter N ^a,b  

^a Case Western Reserve University   (United States)

^b VANDERBILT UNIVERSITY   (United States)

Author keywords

Cell encapsulation; Hydrogel; Mechanical properties; Photopolymerization; Tissue engineering

Indexed keywords

BLEND COMPOSITION; CELL ENCAPSULATION; CELL ENCAPSULATIONS; CELL VIABILITY; COMPRESSIVE MODULI; CONCENTRATION OF; CROSSLINKED; FLUORESCENCE ASSAY; HIGH MOLECULAR WEIGHT; HIGHER-DEGREE; MECHANICAL BEHAVIOR; POLYETHYLENE GLYCOL DIACRYLATE; POLYMER CONCENTRATIONS; ROBUST SCAFFOLD; TISSUE ENGINEERING APPLICATIONS; TISSUE ENGINEERING SCAFFOLD; WEIGHT PERCENT;

BIOCOMPATIBILITY; COMPRESSIVE STRENGTH; CONCENTRATION (PROCESS); ETHYLENE; ETHYLENE GLYCOL; HYDROGELS; MECHANICAL PROPERTIES; MOLECULAR WEIGHT; PHOTOPOLYMERIZATION; POLYETHYLENE GLYCOLS; POLYETHYLENE OXIDES; POLYMER BLENDS; SCAFFOLDS; TISSUE ENGINEERING;

BIOMECHANICS;

CHEMICAL AGENT; POLYETHYLENE GLYCOL DIACRYLATE; POLYMER; TISSUE SCAFFOLD; UNCLASSIFIED DRUG;

ARTICLE; CELL VIABILITY; COMPRESSIVE STRENGTH; CROSS LINKING; ELASTICITY; FLUORESCENCE ANALYSIS; HUMAN; HUMAN CELL; MOLECULAR WEIGHT; TISSUE ENGINEERING;

ACRYLATES; BIOCOMPATIBLE MATERIALS; CELL SURVIVAL; COMPRESSIVE STRENGTH; CROSS-LINKING REAGENTS; ELASTICITY; HUMANS; MATERIALS TESTING; MOLECULAR WEIGHT; POLYETHYLENE GLYCOLS; POLYMERS; STRESS, MECHANICAL; TISSUE ENGINEERING; TISSUE SCAFFOLDS;

EID: 77950275414     PISSN: 15493296     EISSN: 15524965     Source Type: Journal    
DOI: 10.1002/jbm.a.32563     Document Type: Article

References (30)

1. Hahn M, Miller JS, West JL. Three-dimensional biochemical and biomechanical patterning of hydrogels for guiding cellular behavior. Adv Mater 2006;18:2679-2685.
2. Patel P, Smith CK. Rheological and recovery properties of poly(ethylene glycol) diacrylate hydrogels and human adipose tissue. J Biomed Mater Res A 2005;73A:313-319.
3. Arcaute K, Mann BK, Wicker RB. Stereolithography of threedimensional bioactive poly(ethylene glycol) constructs with encapsulated cells. Ann Biomed Eng 2006;34:1429-1441.
4. Cruise G, Scharp DS, Hubbell JA. Characterization of permeability and network structure of interfacially photopolymerized poly(ethylene glycol) diacrylate hydrogels. Biomaterials 1998;19:1287-1294.
5. Halstenberg S, Panitch A, Rizzi S, Hall H, Hubbell J. Biologically engineered protein-graft-poly(ethylene glycol) hydrogels: A cell adhesive and plasmin-degradable biosynthetic material for tissue repair. Biomacromolecules 2002;3:710-723.
6. Bikram M, Fouletier-Dilling C, Gobin AM, Davis AR, Olmsted-Davis EA, West JL. Hydrogel encapsulation of adenovirus-transduced cells expressing BMP2 for local oseteoinduction. Mol Ther 2005;11L:S263-S264.
7. Ghosh K, Ren XD, Shu XZ, Prestwich GD, Clark RA. Fibronectin functional domains coupled to hyaluronan to stimulate adult human dermal fibroblast responses critical for wound healing. Tissue Eng 2006;12:601-613.
8. Ji Y, Ghosh K, Shu XZ, Bingquan L, Sokolov JC, Prestwich GD, Clark RAF, Rafailovich MH. Electrospun three-dimensional hyaluronic acid nanofibrous scaffolds. Biomaterials 2006;27:3782-3792.
9. Hahn S, Taite LJ, Moon JJ, Rowland MC, Ruffino KA, West JL. Photolithographic patterning of polyethylene glycol hydrogels. Biomaterials 2006;27:2519-2524.
10. Seidell J, Malonge SM. Synthesis of polyHEMA hydrogels for use as biomaterials; bulk and solution initiated polymerization techniques. Mater Res 2000;3:79-83.
11. Shu XZ, Ahmad S, Liu Y, Prestwich GD. Synthesis and evaluation of injectable, in situ, crosslinkable synthetic extracellular matrices (sECMs) for tissue engineering applications. J Biomed Mater Res A 2006;79A:901-912.
12. Nguyen KT, West JL. Photopolymerizable hydrogels for tissue engineering applications. Biomaterials 2002;23:4307-4314.
13. Antonio X, Gaydos C, Mao JJ. In vitro degradation behavior of photopolymerized PEG hydrogels as a tissue engineering scaffold. In Proceedings of the 28th IEEE EMBS Annual International Conference, IEEE, New York, 2006.
14. Guoxin T, Yingjun W, Guang Y. Synthesis and properties of polyethylene glycol diacrylate 2-hydroxyethyl methacrylate hydrogels. Key Eng Mater 2008;368-372:1175-1177.
15. Saltzman W. Tissue Engineering. New York: Oxford University Press; 2004. p 400.
16. Valles E, Durando D, Katime I, Mendizabal E, Puig JE. Equilibrium swelling and mechanical properties of hydrogels. Polym Bull 2000;44:109-114.
17. Butler M, Clark MH, Adams S. Swelling and mechanical behavior of biopolymer hydrogels containing chitosan and bovine serum albumin. Biomarcomolecules 2006;7:2961-2970.
18. Barcellos O, Pires ATN, Katime I. Physical properties of hydrogels of poly(2-hydroxyethyl methacrylate) and copolymers with mono-methyl itaconate synthesized by bulk and solution polymerization. Polym Int 2000;49:825-830.
19. Bryant S, Nuttelman CR, Anseth KS. Cytocompatiblity of UV and visible light photoinitiating systems on cultured NIH/ 3T3 fibroblasts in vitro. J Biomater Sci Polym Ed 2000;11:439-457.
20. Riley W, Sturges DL, Morris DH. Statics and Mechanics of Materials: An Integrated Approach. New York: John Wiley; 1996. p 91.
21. Montgomery D. Design and Analysis of Experiments, 6th ed. New York: John Wiley; 2005. p 94.
22. Sperling L. Introduction to Physical Polymer Science, 2nd ed. New York: John Wiley; 1992. p 382-401.
23. Schmedlen R, Nyugen KT, West JL. Tissue engineered vascular grafts: Elastic polyethylene glycol scaffolds for culture under pulsatile flow conditions. In Proceedings of the Second Joint EMBS/BMES Conference, Houston, 2002.
24. Flory P. Principles of Polymer Chemistry. London: Cornell University Press; 1969. p 433.
25. Bryant SJ, Anseth KS, Lee DA, Bader DL. Crosslinking density influences the morphology of chondrocytes photoencapsulated in PEG hydrogels during the application of compressive strain. J Orthop Res 2004;22:1143-1149.
26. Liao H, Munoz-Pinto D, Qu X, Hou Y, Grunlan MA, Hahn MS. Influence of hydrogel mechanical properties and mesh size on vocal fold fibroblast extracellular matrix production and phenotype. Acta Biomater 2008;4:1161-1171.
27. Saltzman W. Tissue Engineering. Oxford: Oxford University Press; 2004. p 348-349.
28. Eckstein F, Lemberger B, Gratzke C, Hudelmaier M, Glaser C, Englmeier K, Reiser M. In vivo cartilage deformation after different types of activity and its dependence on physical training status. Ann Rheum Dis 2005;64:291-295.
29. Burdick JA, Chung C, Xinqiao J, Randolph MA, Langer R. Controlled degradation and mechanical behavior of photopolymerized hyaluronic acid networks. Biomacromolecules 2006;6:386-391.
30. Reynolds C, Tedeschi H. Permeability properties of mammalian cell nuclei in living cells and in vitro. J Cell Sci 1984;70:197-207.