DAS181, a sialidase fusion protein, protects human airway epithelium against influenza virus infection: An in vitro pharmacodynamic analysis

Journal of Antimicrobial Chemotherapy

Volumn 65, Issue 2, 2009, Pages 275-284

  Triana Baltzer, Gallen B ^a   Babizki, Maria ^a   Chan, Michael C W ^b   Wong, Adam C N ^b   Aschenbrenner, Laura M ^a   Campbell, Erin R ^a   Li, Qi Xiang ^a   Chan, Renee W Y ^b   Peiris, J S Malik ^b   Nicholls, John M ^b   Fang, Fang ^a  

^a NexBio Inc   (United States)

^b UNIVERSITY OF HONG KONG   (Hong Kong)

Author keywords

Ex vivo bronchi; Fludase; HAE

Indexed keywords

AMPHIREGULIN; ANTIVIRUS AGENT; DAS 181; FLUDASE; SIALIC ACID; UNCLASSIFIED DRUG;

ANTIVIRAL ACTIVITY; ARTICLE; BRONCHUS; CONTROLLED STUDY; DRUG EFFICACY; DRUG POTENCY; DRUG PROTEIN BINDING; EX VIVO STUDY; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VITRO STUDY; INFLUENZA; PHARMACODYNAMICS; PROTEIN DOMAIN; RESPIRATORY EPITHELIUM; SIALYLATION; TISSUE REGENERATION; VIRUS CULTURE; VIRUS REPLICATION; VIRUS STRAIN;

ANTIVIRAL AGENTS; CELLS, CULTURED; EPITHELIUM; HUMANS; INFLUENZA, HUMAN; ORGAN CULTURE TECHNIQUES; ORTHOMYXOVIRIDAE; RECOMBINANT FUSION PROTEINS;

EID: 77950253495     PISSN: 03057453     EISSN: 14602091     Source Type: Journal    
DOI: 10.1093/jac/dkp421     Document Type: Article

References (25)

1. Englund JA. Antiviral therapy of influenza. Semin Pediatr Infect Dis 2002; 13: 120-8.
2. Reece PA. Neuraminidase inhibitor resistance in influenza viruses. J Med Virol 2007; 79: 1577-86.
3. Nicholls JM, Chan RW, Russell RJ et al. Evolving complexities of influenza virus and its receptors. Trends Microbiol 2008; 16: 149-57.
4. Malakhov MP, Aschenbrenner LM, Smee DF et al. Sialidase fusion protein as a novel broad-spectrum inhibitor of influenza virus infection. Antimicrob Agents Chemother 2006; 50: 1470-9.
5. Belser JA, Lu X, Szretter KJ et al. DAS181, a novel sialidase fusion protein, protects mice from lethal avian influenza H5N1 virus infection. J Infect Dis 2007; 196: 1493-9.
6. Cheung CY, Poon LL, Lau AS et al. Induction of proinflammatory cytokines in human macrophages by influenza A (H5N1) viruses: a mechanism for the unusual severity of human disease? Lancet 2002; 360: 1831-7.
7. Nicholls JM, Chan MC, Chan WY et al. Tropism of avian influenza A (H5N1) in the upper and lower respiratory tract. Nat Med 2007; 13: 147-9.
8. Ward CL, Dempsey MH, Ring CJ et al. Design and performance testing of quantitative real time PCR assays for influenza A and B viral load measurement. J Clin Virol 2004; 29: 179-88.
9. 2δδC(T) method. Methods 2001; 25: 402-8.
10. Li Z, Liu B, Maminishkis A et al. Gene expression profiling in autoimmune noninfectious uveitis disease. J Immunol 2008; 181: 5147-57.
11. Nicholls JM, Aschenbrenner LM, Paulson JC et al. Comment on: Concerns of using sialidase fusion protein as an experimental drug to combat seasonal and pandemic influenza. J Antimicrob Chemother 2008; 62: 426-8.
12. Shinya K, Ebina M, Yamada S et al. Avian flu: influenza virus receptors in the human airway. Nature 2006; 440: 435-6.
13. Brydon EW, Morris SJ, Sweet C. Role of apoptosis and cytokines in influenza virus morbidity. FEMS Microbiol Rev 2005; 29: 837-50.
14. Lowy RJ. Influenza virus induction of apoptosis by intrinsic and extrinsic mechanisms. Int Rev Immunol 2003; 22: 425-49.
15. Hayden FG, Fritz R, Lobo MC et al. Local and systemic cytokine responses during experimental human influenza A virus infection. Relation to symptom formation and host defense. J Clin Invest 1998; 101: 643-9.
16. Reichner JS, Whiteheart SW, Hart GW. Intracellular trafficking of cell surface sialoglycoconjugates. J Biol Chem 1988; 263: 16316-26.
17. Gong JH, Sprenger H, Hinder F et al. Influenza A virus infection of macrophages. Enhanced tumor necrosis factor-alpha (TNF-alpha) gene expression and lipopolysaccharide-triggered TNF-alpha release. J Immunol 1991; 147: 3507-13.
18. Matsukura S, Kokubu F, Noda H et al. Expression of IL-6, IL-8, and RANTES on human bronchial epithelial cells, NCI-H292, induced by influenza virus A. J Allergy Clin Immunol 1996; 98: 1080-7.
19. Peschke T, Bender A, Nain M et al. Role of macrophage cytokines in influenza A virus infections. Immunobiology 1993; 189: 340-55.
20. Matrosovich MN, Matrosovich TY, Gray T et al. Neuraminidase is important for the initiation of influenza virus infection in human airway epithelium. J Virol 2004; 78: 12665-7.
21. Bocharov GA, Romanyukha AA. Mathematical model of antiviral immune response. III. Influenza A virus infection. J Theor Biol 1994; 167: 323-60.
22. Chang DB, Young CS. Simple scaling laws for influenza A rise time, duration, and severity. J Theor Biol 2007; 246: 621-35.
23. Hancioglu B, Swigon D, Clermont G. A dynamical model of human immune response to influenza A virus infection. J Theor Biol 2007; 246: 70-86.
24. Thompson CI, Barclay WS, Zambon MC et al. Infection of human airway epithelium by human and avian strains of influenza a virus. J Virol 2006; 80: 8060-8.
25. Chan RW, Chan MC, Wong AC et al. DAS181 inhibits H5N1 influenza virus infection of human lung tissues. Antimicrob Agents Chemother 2009; 53: 3935-41.