Detection of Aspergillus fumigatus by quantitative polymerase chain reaction in air samples impacted on low-melt agar

American Journal of Infection Control

Volumn 38, Issue 3, 2010, Pages 195-198

  Bellanger, Anne Pauline ^a,b   Reboux, Gabriel ^a,b   Murat, Jean Benjamin ^a,b   Bex, Valerie ^c   Millon, Laurence ^a,b  

^a UNIVERSITY HOSPITAL JEAN MINJOZ   (France)

^b UNIVERSITÉ DE FRANCHE COMTÉ   (France)

^c Paris Hygiene Laboratory   (France)

Author keywords

Aspergillus fumigatus; environmental sampling; fungal surveillance; immunocompromised patients; QPCR

Indexed keywords

AGAR; FUNGAL DNA;

AIR SAMPLING; AIRBORNE FUNGUS; ARTICLE; ASPERGILLUS FUMIGATUS; ASPERGILLUS NIGER; BASIDIOMYCETES; CONIDIUM; CONTROLLED STUDY; DNA EXTRACTION; FUNGAL DETECTION; FUNGUS CULTURE; FUNGUS IDENTIFICATION; MOLECULAR PROBE; NONHUMAN; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS;

AGAR; AIR MICROBIOLOGY; ASPERGILLUS FUMIGATUS; BACTERIOLOGICAL TECHNIQUES; CULTURE MEDIA; HUMANS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TIME FACTORS;

EID: 77949914481     PISSN: 01966553     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ajic.2009.08.003     Document Type: Article

References (19)

1. Marr K.A., Carter R.A., Crippa F., Wald A., and Corey L. Epidemiology and outcome of mould infections in hematopoietic stem cell transplant recipients. Clin Infect Dis 34 (2002) 909-917
2. Walsh T.J., Anaissie E.J., Denning D.W., Herbrecht R., Kontoyiannis D.P., Marr K.A., et al. Treatment of aspergillosis: clinical practice guidelines of the Infectious Diseases of America. Clin Infect Dis 46 (2008) 327-360
3. Perfect J.R., Cox G.M., Lee J.Y., Kauffman C.A., de Repentigny L., and Chapman S.W. The impact of culture isolation of Aspergillus species: a hospital-based survey of aspergillosis. Clin Infect Dis 33 (2001) 1824-1833
4. Falvey D.G., and Streifel A.J. Ten-year air sample analysis of Aspergillus prevalence in a university hospital. J Hosp Infect 67 (2007) 35-41
5. Rupp M.E., Iwen P.C., Tyner L.K., Marion N., Reed E., and Anderson J.R. Routine sampling of air for fungi does not predict risk of invasive aspergillosis in immunocompromised patients. J Hosp Infect 68 (2008) 270-271
6. Morrison J., Yang C., Lin K.T., Haugland R.A., Neely A.N., and Vesper A.J. Monitoring Aspergillus species by quantitative PCR during construction of a multi-story hospital building. J Hosp Infect 57 (2004) 85-87
7. Chang C.C., Cheng A.C., Devitt B., Hughes A.J., Campbell P., Styles K., et al. Successful control of an outbreak of invasive aspergillosis in a regional haematology unit during hospital construction works. J Hosp Infect 69 (2008) 33-38
8. Haugland R.A., Varma M., Wymer L.J., and Vesper S.J. Quantitative PCR analysis of selected Aspergillus, Penicillium, and Paecilomyces species. Syst Appl Microbiol 27 (2004) 198-210
9. Vadrot C., Bex V., Mouilleseaux A., Squinazi F., and Darbord J.C. Detection of Mycobacterium tuberculosis complex by PCR in hospital air samples. J Hosp Infect 58 (2004) 262-267
10. Keswani J., Kashon M.L., and Chen B.T. Evaluation of interference to conventional and real-time PCR for detection and quantification of fungi in dust. J Environ Monit 7 (2005) 311-318
11. Vesper S.J., Haugland R.A., Rogers M.E., and Neely A.N. Opportunistic Aspergillus pathogens measured in home and hospital tap water by quantitative PCR (QPCR). J Water Health 5 (2007) 427-431
12. Neely A.N., Gallardo V., Barth E., Haugland R.A., Warden G.D., and Vesper S.J. Rapid monitoring by quantitative polymerase chain reaction for pathogenic Aspergillus during carpet removal from a hospital. Infect Control Hosp Epidemiol 25 (2004) 350-352
13. Cruz P., and Buttner M.P. Analysis of bioaerosol samples. In: Hurst C.J., Crawford R.L., Garland J.L., Lipson D.A., Mills A.L., and Stetzenbach L.D. (Eds). Manual of environmental microbiology. 3rd ed (2007), ASM Press, Washington, DC 952-960
14. + T-cell response to live and heat-inactivated Aspergillus fumigatus conidia. Infect Immun 73 (2005) 7170-7179
15. Wasylnka J.A., Hissen A.H., Wan A.N., and Moore M.M. Intracellular and extracellular growth of Aspergillus fumigatus. Med Mycol 43 Suppl 1 (2005) S27-S30
16. Vesper S., McKinstry C., Hartmann C., Neace M., Yoder S., and Vesper A. Quantifying fungal viability in air and water samples using quantitative PCR after treatment with propidium monoazide (PMA). J Microbiol Methods 72 (2008) 180-184
17. Spicer R., and Gangloff H. Establishing site-specific reference levels for fungi in outdoor air for building evaluation. J Occup Environ Hyg 2 (2005) 257-266
18. Spicer R.C., and Gangloff H.J. Bioaerosol data distribution: probability and implications for sampling in evaluating problematic buildings. Appl Occup Environ Hyg 18 (2003) 584-590
19. Vesper S.J., Wong W., Kuo C.M., and Pierson D.L. Mold species in dust from the International Space Station identified and quantified by mold-specific quantitative PCR. Res Microbiol 159 (2008) 432-435