DNA extraction from coagulated human blood for application in genotyping techniques for human leukocyte antigen and immunoglobulin-like receptors;Extração de DNA a partir de sangue humano coagulado para aplicação nas técnicas de genotipagem de antígenos leucocitários humanos e de receptores semelhantes à imunoglobulina

Revista da Sociedade Brasileira de Medicina Tropical

Volumn 42, Issue 6, 2009, Pages 651-656

  Cardozo, Daniela Maira ^a   Guelsin, Gláucia Andréia ^a   Clementino, Samaia Laface ^a   De Melo, Fabiano Cavalcante ^a   Braga, Marco Antônio ^a   De Souza, Cleonice ^b   Moliterno, Ricardo Alberto ^a   Visentainer, Jeane Eliete Laguila ^a  

^a STATE UNIVERSITY OF MARINGÁ   (Brazil)

^b Secretaria de Saude do Parana   (Brazil)

Author keywords

Coagulated blood; DNA extraction; Molecular biology; Standardization

Indexed keywords

DNA;

ARTICLE; BLOOD; COAGULATED BLOOD; DNA EXTRACTION; GENE; GENOTYPE; HUMAN; HUMAN LEUKOCYTE ANTIGEN GENE; KILLER CELL IMMUNOGLOBULIN LIKE RECEPTOR GENE; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS;

DNA; GENOTYPE; HLA ANTIGENS; HUMANS; LUMINESCENT MEASUREMENTS; POLYMERASE CHAIN REACTION; RECEPTORS, KIR;

EID: 77949838449     PISSN: 00378682     EISSN: None     Source Type: Journal    
DOI: 10.1590/S0037-86822009000600008     Document Type: Article

References (6)

1. Adkins KK, Strom DA, Jacobson TE, Seemann CR, O'Brien DP, Heath EM. Utilizing Genomic DNA Purified From Clotted Blood Samples for Single Nucleotide Polymorphism Genotyping. Archives of Pathology and Laboratory Medicine 126:266-270, 2002.
2. Everson RB, Mass MJ, Gallagher JE, Musser C, Dalzell J. Extraction of DNA from Cryopreserved Clotted Human Blood. Biotechniques 15:18-20, 1993.
3. Garg UC, Hanson NQ, Tsai MY, Eckfeldt JH. Simple and Rapid Method for Extraction of DNA from fresh and Cryopreserved Clotted Human Blood. Clinical Chemistry 42:647-648, 1996.
4. John SW, Weitzner G, Rozen R, Scriver CR. A rapid procedure for extracting genomic DNA from leukocytes. Nucleic Acids Research 192: 408-408, 1991.
5. Lahiri DK, Nurnberger JI Jr. A rapid non-enzymatic method for the preparation of HMW DNA from blood for RFLP studies. Nucleic Acids Research 19: 5444-5444, 1991.
6. Zeillinger R, Schneeberger C, Speiser P, Kury F. A Simple Method for Isolation of DNA from Blood Clots Suited for use in PCR. Biotechniques 14:202-203, 1993.