Buckwheat (Fagopyrum esculentum Moench) FeMT3 gene in heavy metal Stress: Protective role of the protein and inducibility of the promoter region under Cu2+ and Cd2+ treatments

Journal of Agricultural and Food Chemistry

Volumn 58, Issue 6, 2010, Pages 3488-3494

  Nikolić, Dragana B ^a   Samardžić, Jelena T ^a   Bratić, Ana M ^a,c   Radin, Ivan P ^a   Gavrilović, Srdjan P ^a,d   Rausch, Thomas ^b   Maksimović, Vesna R ^a  

^a UNIVERSITY OF BELGRADE   (Serbia)

^b UNIVERSITY OF HEIDELBERG   (Germany)

^c KAROLINSKA INSTITUTE   (Sweden)

^d Centre for Carbohydrate Recognition and Signalling   (Denmark)

Author keywords

Buckwheat (fagopyrum esculentum moench); Heavy metal tolerance; Metallothionein; Overexpression

Indexed keywords

CADMIUM; COPPER; GROWTH INHIBITOR; HEAVY METAL; NERVE PROTEIN; VEGETABLE PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; FAGOPYRUM; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MOLECULAR GENETICS; PROMOTER REGION; SEQUENCE ALIGNMENT; TOBACCO;

AMINO ACID SEQUENCE; CADMIUM; COPPER; FAGOPYRUM; GENE EXPRESSION REGULATION, PLANT; METALS, HEAVY; MOLECULAR SEQUENCE DATA; NERVE TISSUE PROTEINS; PLANT PROTEINS; PROMOTER REGIONS, GENETIC; SEQUENCE ALIGNMENT; TOBACCO;

ESCHERICHIA COLI; FAGOPYRUM ESCULENTUM; NICOTIANA;

EID: 77949820670     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/jf904483a     Document Type: Article

References (30)

1. Schutzendubel, A.; Pole, A. Plant responses to abiotic stresses: Heavy metal-induced oxidative stress and protection by mycorrhizatinn. J. Exp. Bot. 2002. 53. 1351-1365.
2. Eapen, S.; D'Souza, S. F. Prospects of genetic engineering of plants for phytoremediation of toxic metals. Biotechnol.Adv.- 2005, 23, 97-114.
3. Shen, R.; Ma, J. F.; Kyo, M.; Iwashita, T. Compartmentation of aluminium in leaves of an Al-accumulator, Fagopyrum esculentum Moench. Planta 2002, 215, 394-398.
4. Tani, F. H.; Barrington, S. Zinc and copper uptake by plants under two transpiration rates. Part II. Buckwheat (Fagopyrum esculentum L.). Environ. Pollut. 2005, 138, 548-558.
5. Tamura, H.; Honda, M.; Sato, T.; Kamachi, H. Pb hyperaccumulation and tolerance in common buckwheat (Fagopyrum esculentum Moench). J., Plant Res. 2005, 118, 355-359.
6. Gorinova, N.; Nedkovska, M.; Todorovska, E.; Simova-Stoilova, L.; Stoyanova, Z.; Georgieva, K.; Demirevska-Kepova, K.; Atanassov, A.; Herzig, R. Improved phytoaccumulation of cadmium by genetically modified tobacco plants (Nicotiana tabacum L.). Physiological and biochemical response of the transformants to cadmium toxicity. Environ, Pollut, 2007, 145, 161-170.
7. Qi, X.; Zhang, Y.; Chai, T. Characterization of a novel plant promoter specifically induced by heavy metal and identification of the promoter regions conferring heavy metal responsiveness. Plant Phvsiol. 2007,143, 50-59.
8. Cobbett, C.; Goldsbrough, P. Phytochelatins and metallothioneins: Roles in heavy metal detoxification and homeostasis. Annu. Rev. Plant Biol. 2002, 53, 159-182.
9. Xue, T.; Li, X.; Zhu, W.; Wu, C.; Yang, G.; Zheng, C. Cotton metallothionein GhMT3a, a reactive oxygen species scavenger, increased tolerance against abiotic stress in transgenic tobacco and yeast. J. EXP. Hot. 2009, 60, 339-349.
10. Lee, J.; Shim, D.; Song, W. Y.; Hwang, I.; Lee, Y. Arabidopsis metallothioneins 2a and 3 enhance resistance to cadmium when expressed in Vicia faba guard cells. Plant Mol. Biol. 2004, 54, 805-815.
11. Guo, W. J.; Meetam, M.; Goldsbrough, P. B. Examining the specific contributions of individual Arabidopsis metallothioneins to copper distribution and metal tolerance. Plant Physiol. 2008, 146,1697-1706.
12. Zhou, G. K.; Xu, Y. F.; Liu, J. Y. Characterization of a rice class II metallothionein gene: tissue expression patterns and induction in response to abiotic factors. J. Plant Physiol. 2005,162, 686-696.
13. Brkljačić, J. M.; Samardžić, J. T.; Timotijević, G. S.; Maksimović, V. R. Expression analysis of buckwheat (Fagopyrum esculentum Moench) metallothionein-like gene (MT3) under different stress and physiological conditions. J. Plant Physiol. 2004,161, 741-746.
14. Bratić, A. M.; Majić, D. B.; Samardžić, J. T.; Maksimović, V. R. Functional analysis of the buckwheat metallothionein promoter: Stimuli specificity pattern and up-regulation under complex stress stimuli. J. Plant. Physiol. 2009, 166, 996-1000.
15. Jefferson, R.; Kavanagh, T.; Bevan, M. GUS fusions: Beta-glucuronidase activity as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 1987, 6, 3901-3907.
16. Timotijevic, G. S.; Milisavljevic, M. D.; Radovic, S. R.; Konstantinovic, M. M.; Maksimovic, V. R. Ubiquitous aspartic proteinase as an actor in the stress response in buckwheat. .J. Plant Phvsiol. 2010, 167, 61-68.
17. Domenech, J.; Orihuela, R.; Mir, G.; Molinas, M.; Atrian, S.; Capdevila, M. The Cd(II)-binding abilities of recombinant Quercus suber metallothionein: Bridging the gap between phytochelatins and metallothioneins. ,J. Biol. Inore. Chem. 2007,12, 867-882.
18. Costa, H.; Gallego, S. M.; Tomaro, M. L. Effect of UV-B radiation on antioxidant defense system in sunflower cotyledons. Plant Sci. 2002,162, 939-945.
19. Ritchie, R. J. Consistent sets of spectrophotometric chlorophyll equations for acetone, methanol and ethanol solvents. Photosynth. Res. 2006, 89,27-41.
20. Brkljačić, J.; Majić, D.; Miljuš- Djukić, J.; Bratić, A.; Konstantinović, M.; Milisavljević, M.; Maksimović, V. The metallothionein-like gene from, buckwheat-Structural and functional analysis of the promoter region. Int. J. Plant Sci. 2005,166, 889-900.
21. Majic, D. B.; Samardzic, J. T.; Milisavljevic, M. D.; Krstic, A. M.; Maksimovic, V. R. Two metallothionein gene family members in buckwheat: expression analysis in flooding stress using real time RT-PCR technology. Arch., Biol, Sci. 2008, 60, 77-82.
22. Thiele, D. J.; Wright, C. F.; Walling, M. J.; Hamer, D. H. Function and regulation of yeast copperthionein. Experientia Suppl. 1987, 52, 423-429.
23. Zhigang, A.; Cuijie, L.; Yuangang, Z.; Yejie, D.; Wachter, A.; Gromes, R.; Rausch, T. Expression of BJMT2, a metallothionein 2 from Brassica juncea, increases copper and cadmium tolerance in Escherichia coli and Arabidopsis thaliana, but inhibits root elongation in Arabidopsis thaliana seedlings. J. Exp. Bot. 2006, 57, 3575-3582.
24. Garcia-Hernandez, M.; Murphy, A.; Taiz, L. Metallothioneins 1 and 2 have distinct but overlapping expression patterns in Arabidopsis. Plant. Physiol. 1998, 118, 387-397.
25. Guo, W.-J.; Bundithya, W.; Goldsbrough, P. B. Characterization of the Arabidopsis metallothionein gene family: Tissue-specific expression and induction during senescence and in response to copper. New Phytol. 2003. 159. 369-381.
26. Lu, S.; Gu, H.; Yuan, X.; Wang, X.; Wu, A. M.; Qu, L.; Liu, J. Y. The GUS reporter-aided analysis of the promoter activities of a rice metallothionein gene reveals different regulatory regions responsible for tissue-specific and inducible expression in transgenic Arabidopsis. Transgenic Res. 2007, 16,177-191.
27. Park, J. N.; Sohn, M. J.; Oh, D. B.; Kwon, O.; Rhee, S. K.; Hur, C. G.; Lee, S. Y.; Gellissen, G.; Kang, H. A. Identification of the cadmium-inducible Hansenula polymorpha SEO1 gene promoter by transcriptome analysis and its application to whole-cell heavy-metal detection systems. Appl, Environ., Microbiol. 2007, 73, 5990-6000.
28. Yang, Z.; Wu, Y.; Li, Y.; Ling, H. Q.; Chu, C. OsMT1a, a type 1 metallothionein, plays the pivotal role in zinc homeostasis and drought tolerance in rice. Plant. Mol. Biol. 2009, 70, 219-229.
29. Zimeri, A. M.; Dhankher, O. P.; McCaig, B.; Meagher, R. B. The plant MTl metallothioneins are stabilized by binding cadmiums and are required for cadmium tolerance and accumulation. Plant Mol. Biol. 2005, 58, 839-855.
30. Hassinen, V. H.; Tuomainen, M.; Peraniemi, S.; Schat, H.; Karenlampi, S. O.; Tervahauta, A. I. Metallothioneins 2 and 3 contribute to the metal-adapted phenotype but are not directly linked to Zn accumulation in the metal hyperaccumulator, Thlaspi caerulescens. J. EXP. Bot. 2009, 60, 187-196.