Efficient plant-based production of chicken interleukin-12 Yields a strong immunostimulatory cytokine

Journal of Interferon and Cytokine Research

Volumn 30, Issue 3, 2010, Pages 143-153

  Medrano, Giuliana ^a   Dolan, Maureen C ^a   Stephens, Nathan T ^b   McMickle, Anthony ^a   Erf, Gisela ^c   Radin, David ^b   Cramer, Carole L ^a,b  

^a ARKANSAS STATE UNIVERSITY   (United States)

^b State University   (United States)

^c UNIVERSITY OF ARKANSAS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTINEOPLASTIC AGENT; INTERLEUKIN 12; POLYHISTIDINE TAG; PROTEIN P40; VACCINE;

ARTICLE; BIOLOGICAL ACTIVITY; CELLULAR IMMUNITY; CHICKEN; IMMUNE RESPONSE; IMMUNOSTIMULATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT PRODUCT; PRIORITY JOURNAL; RHIZOBIUM; STOICHIOMETRY; TH1 CELL; TOBACCO; TRANSGENIC PLANT; VETERINARY MEDICINE;

ADJUVANTS, IMMUNOLOGIC; ANIMALS; CELLS, CULTURED; CHICKENS; GLYCOSYLATION; INTERLEUKIN-12; PLANT LEAVES; PROTEIN STABILITY; TOBACCO;

AGROBACTERIUM; MAMMALIA; NICOTIANA OBTUSIFOLIA;

EID: 77949802065     PISSN: 10799907     EISSN: None     Source Type: Journal    
DOI: 10.1089/jir.2009.0040     Document Type: Article

References (38)

1. Ahmad M, Mirza B. 2005. An efficient protocol for transient transformation of intact fruit and transgene expression in citrus. Plant Mol Biol Rep 23:419a-419k.
2. Asif M, Jenkins K, Hilton L, Kimpton W, Bean A, Lowenthal J. 2004. Cytokines as adjuvants for avian vaccines. Immunol Cell Biol 82(6):638-643.
3. Becker D. 1990. Binary vectors which allow the exchange of plant selectable markers and reporter genes. Nucleic Acids Res 18(1):203.
4. Bowen O, Erf G, Anthony N, Wideman R. 2006a. Pulmonary hypertension triggered by lipopolysaccharide in ascites-susceptible and -resistant broilers is not amplified by aminoguanidine, a specifi c inhibitor of inducible nitric oxide synthase. Poult Sci 85(3):528-536.
5. Bowen O, Wideman R, Anthony N, Erf G. 2006b. Variation in the pulmonary hypertensive responsiveness of broilers to lipopolysaccharide and innate variation in nitric oxide production by mononuclear cells. Poult Sci 85(8):1349-1363.
6. Carra G, Gerosa F, Trinchieri G. 2000. Biosynthesis and posttranslational regulation of human IL-12. J Immunol 164(9):4752-4761.
7. Carrington J, Freed D. 1990. Cap-independent enhancement of translation by a plant potyvirus 5′ nontranslated region. J Virol 64(4):1590-1597.
8. Condori J, Medrano G, Sivakumar G, Nair V, Cramer C, Medina-Bolivar F. 2009. Functional characterization of a stilbene synthase gene using a transient expression system in planta. Plant Cell Rep 28(4):589-599.
9. Degen W, van Daal N, van Zuilekom H, Burnside J, Schijns V. 2004. Identifi cation and molecular cloning of functional chicken IL-12. J Immunol 172(7):4371-4380.
10. Domeika K, Berg M, Eloranta M, Alm G. 2002. Porcine interleukin- 12 fusion protein and interleukin-18 in combination induce interferon-gamma production in porcine natural killer and T cells. Vet Immunol Immunopathol 86(1-2):11-21.
11. Gomord V, Faye L. 2004. Posttranslational modifi cation of therapeutic proteins in plants. Curr Opin Plant Biol 7(2):171-181.
12. Gubler U, Chua A, Schoenhaut D, Dwyer C, McComas W, Motyka R, Nabavi N, Wolitzky A, Quinn P, Familletti P. 1991. Coexpression of two distinct genes is required to generate secreted bioactive cytotoxic lymphocyte maturation factor. Proc Natl Acad Sci USA 88(10):4143-4147.
13. Gutierrez-Ortega A, Avila-Moreno F, Saucedo-Arias L, Sanchez-Torres C, Gomez-Lim M. 2004. Expression of a single-chain human interleukin-12 gene in transgenic tobacco plants and functional studies. Biotechnol Bioeng 85(7):734-740.
14. Gutierrez-Ortega A, Sandoval-Montes C, de Olivera-Flores T, Santos-Argumedo L, Gomez-Lim M. 2005. Expression of functional interleukin-12 from mouse in transgenic tomato plants. Transgenic Res 14(6):877-885.
15. Ha S, Chang J, Song M, Suh Y, Jin H, Lee C, Nam G, Choi G, Choi K, Lee S, Kim WB, Sung YC. 2002. Engineering N-glycosylation mutations in IL-12 enhances sustained cytotoxic T lymphocyte responses for DNA immunization. Nat Biotechnol 20(4):381-386.
16. Holsters M, de Waele D, Depicker A, Messens E, Van Montangu M, Schell J. 1978. Transfection and transformation of A. tumefaciens. Mol Gen Genet 163(2):181-187.
17. Jenkins K, Bean A, Lowenthal J. 2007. Avian genomics and the innate immune response to viruses. Cytogenet Genome Res 117(1-4):207-212.
18. Knablein J. 2005. Plant-based expression of biopharmaceuticals. In: Meyers RA, ed. Encyclopedia of molecular cell biology and molecular medicine, 2nd ed, Vol.10. Weinheim: Wiley-VCH Verlag GmbH & Co. KGaA.
19. Kobayashi M, Fitz L, Ryan M, Hewick R, Clark S, Chan S, Loudon R, Sherman F, Perussia B, Trinchieri G. 1989. Identifi cation and purifi cation of natural killer cell stimulatory factor (NKSF), a cytokine with multiple biologic effects on human lymphocytes. J Exp Med 170(3):827-845.
20. Kwon T, Seo J, Kim J, Lee J, Jang Y, Yang M. 2003. Expression and secretion of the heterodimeric protein interleukin-12 in plant cell suspension culture. Biotechnol Bioeng 81(7):870-875.
21. Lambrecht B, Gonze M, Meulemans G, van den Berg TP. 2000. Production of antibodies against chicken interferon-γ: demonstration of neutralizing activity and development of a quantitative ELISA. Vet Immunol Immunopathol 74:137-144.
22. Liu J, Dolan M, Reidy M, Cramer C. 2008. Expression of bioactive single-chain murine IL-12 in transgenic plants. J Interferon Cytokine Res 28(6):381-392.
23. Makabe K, Asano R, Ito T, Tsumoto K, Kudo T, Kumagai I. 2005. Tumor-directed lymphocyte-activating cytokines: refoldingbased preparation of recombinant human interleukin-12 and an antibody variable domain-fused protein by additive-introduced stepwise dialysis. Biochem Biophys Res Commun 328(1):98-105.
24. Medina-Bolivar F, Cramer C. 2004. Production of recombinant proteins by hairy roots cultured in plastic sleeve bioreactors. Methods Mol Biol 267:351-363.
25. Medrano G, Reidy M, Liu J, Ayala J, Dolan M, Cramer C. 2009. Rapid system for evaluating bioproduction capacity of complex pharmaceutical proteins in plants. Methods Mol Biol 483:51-67.
26. Pfaffl MW, Horgan GW, Dempfl e L. 2002. Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res 30(9):e36.
27. Podlaski F, Nanduri V, Hulmes J, Pan Y, Levin W, Danho W, Chizzonite R, Gately M, Stern A. 1992. Molecular characterization of interleukin 12. Arch Biochem Biophys 294(1):230-237.
28. Saint-Jore-Dupas C, Faye L, Gomord V. 2007. From planta to pharma with glycosylation in the toolbox. Trends Biotechnol 25(7):317-323.
29. Schijns V, Wierda C, Vahlenkamp T, Horzinek M. 1997. Molecular cloning of cat interleukin-12. Immunogenetics 45(6):462-463.
30. Sheludko Y, Sindarovska Y, Gerasymenko I, Bannikova M, Kuchuk N. 2007. Comparison of several Nicotiana species as hosts for highscale Agrobacterium-mediated transient expression. Biotechnol Bioeng 96(3):608-614.
31. Stern A, Podlaski F, Hulmes J, Pan Y, Quinn P, Wolitzky A, Familletti P, Stremlo D, Truitt T, Chizzonite R. 1990. Purifi cation to homogeneity and partial characterization of cytotoxic lymphocyte maturation factor from human B-lymphoblastoid cells. Proc Natl Acad Sci USA 87(17):6808-6812.
32. Stoger E, Sack M, Fischer R, Christou P. 2002. Plantibodies: applications, advantages and bottlenecks. Curr Opin Biotechnol 13(2):161-166.
33. Thomas J, Morris K, Godfrey D, Lowenthal J, Bean A. 2008. Expression, purifi cation and characterisation of recombinant Escherichia coli derived chicken interleukin-12. Vet Immunol Immunopathol 126(3-4):403-406.
34. Trinchieri G. 2003. Interleukin-12 and the regulation of innate resistance and adaptive immunity. Nat Rev Immunol 3(2):133-146.
35. Twyman R, Schillberg S, Fischer R. 2005. Transgenic plants in the biopharmaceutical market. Expert Opin Emerg Drugs 10(1):185-218.
36. Webster R, Hulse D. 2005. Controlling avian fl u at the source. Nature 435(7041):415-416.
37. Wolf S, Temple P, Kobayashi M, Young D, Dicig M, Lowe L, Dzialo R, Fitz L, Ferenz C, Hewick R. 1991. Cloning of cDNA for natural killer cell stimulatory factor, a heterodimeric cytokine with multiple biologic effects on T and natural killer cells. J Immunol 146(9):3074-3081.
38. Wydro M, Kozubek E, Lehmann P. 2006. Optimization of transient Agrobacterium-mediated gene expression system in leaves of Nicotiana benthamiana. Acta Biochim Pol 53(2):289-298.