A novel combination of TaqMan RT-PCR and a suspension microarray assay for the detection and species identification of pestiviruses

Veterinary Microbiology

Volumn 142, Issue 1-2, 2010, Pages 81-86

  LeBlanc, Neil ^a,b   Leijon, Mikael ^a   Jobs, Magnus ^b,c   Blomberg, Jonas ^b   Belák, Sándor ^a  

^a SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

^b UPPSALA UNIVERSITY   (Sweden)

^c Högskolan Dalarna ^*  (Sweden)

Author keywords

BDV; BVDV1; BVDV2; CSFV; Pestivirus; Suspension microarray; TaqMan real time PCR

Indexed keywords

ARTICLE; BOVINE DIARRHEA VIRUS; GENUS; MICROARRAY ANALYSIS; NONHUMAN; PESTIVIRUS; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VIRUS IDENTIFICATION; VIRUS STRAIN;

ANIMALS; CATTLE; CATTLE DISEASES; DNA PRIMERS; MICROARRAY ANALYSIS; PESTIVIRUS; PESTIVIRUS INFECTIONS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SWINE; SWINE DISEASES;

BORDER DISEASE VIRUS; BORNA DISEASE VIRUS; BOVINAE; CLASSICAL SWINE FEVER VIRUS; PESTIVIRUS; SUIDAE; SUS SCROFA; SUS SCROFA DOMESTICA;

EID: 77949659467     PISSN: 03781135     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetmic.2009.09.046     Document Type: Article

References (18)

1. Becher P., Avalos-Ramirez R., Orlich M., Cedillo-Rosales S., Konig M., Schweizer M., Stalder H., Schirrmeier H., Thiel H.-J. Genetic characterization of novel pestivirus genotypes: implications for classification. Virology 2003, 311:96-104.
2. Deregt D., Gilbert S.A., Dudas S., Pasick J., Baxi S., Burton K.M., Baxi M.K. A multiplex DNS suspension microarray for simultaneous detection and differentiation of classical swine fever and other pestiviruses. J. Virol. Methods 2006, 136:17-23.
3. ® xMAP™ technology for rapid, high-throughput multiplexed nucleic acid detection. Clin. Chim. Acta 2006, 363:71-82.
4. Elvander M., Baule C., Persson M., Egyed L., Ballagi-Pordány A., Belák S., Alenius S. An experimental study of a concurrent primary infection with bovine respiratory syncytial virus (BRSV) and bovine viral diarrhoea virus (BVDV) in calves. Acta Vet. Scand. 1998, 39:251-264.
5. Hakhverdyan M., Rasmussen T.B., Thorén P., Uttenthal Å., Belák S. Development of a real-time PCR assay based on primer-probe energy transfer for the detection of swine vesicular disease virus. Arch. Virol. 2006, 151:2365-2376.
6. Hoffman B., Beer M., Schelp C., Schirrmeier H., Depner K. Validation of a real-time RT-PCR assay for sensitive and specific detection of classical swine fever. J. Virol. Methods 2005, 130:36-44.
7. Houe H. Epidemiology of bovine viral diarrhoea virus. Vet. Clin. North Am.: Food Anim. Pract. 1995, 11:521-547.
8. Liu L., Xia H., Belák S., Baule C. A TaqMan real-time RT-PCR assay for selective detection of recently described bovine atypical pestiviruses in clinical samples and biological products. J. Virol. Methods 2008, 154:82-85.
9. McKillen J., Hjertner B., Millar A., McNeilly F., Belák S., Adair B., Allan G. Molecular beacon real-time PCR detection of swine viruses. J. Virol. Methods 2006, 140:155-165.
10. Moennig V., Plagemann G.W. The pestiviruses. Adv. Virus Res. 1992, 41:53-98.
11. Molenkamp R., van der Ham A., Schinkel J., Beld M. Simultaneous detection of five different DNA targets by real-time TaqMan PCR using the Roche LightCycler480: application in viral molecular diagnostics. J. Virol. Methods 2007, 141:205-211.
12. Nettleton P.F., Gilray J.A., Russo P., Dlissi E. Border disease of sheep and goats. Vet. Res. 1998, 29:327-340.
13. Pluimers F.H., de Leeuw P.W., Smak J.A., Elbers A.R., Stegeman J.A. Classical swine fever in The Netherlands 1997-1998: a description of organisation and measures to eradicate the disease. Prev. Vet. Med. 1999, 42:139-155.
14. Potgieter L.N.D. Immunology of bovine viral diarrhea virus. Vet. Clin. North Am.: Food Anim. Pract. 1995, 11:501-520.
15. Ståhl K., Kampa J., Alenius S., PerssonWadman A., Baule C., Aiumlamai S., Belák S. Natural infection of cattle with an atypical 'HoBi'-like pestivirus-implications for BVD control and for the safety of biological products. Vet. Res. 2007, 38:517-523.
16. Terpstra C., de Smit A.J. The 1997/1998 epizootic of swine fever in the Netherlands: control strategies under a non-vaccination regimen. Vet. Microbiol. 2000, 77:3-15.
17. Vet J., Majithia A.R., Marras S., Tyagi S., Dube S., Poiesz B.J., Kramer F.R. Multiplex detection of four pathogenic retroviruses using molecular beacons. Proc. Natl. Acad. Sci. U.S.A. 1999, 96(11):6394-6399.
18. Vilcek S., Herring A.J., Herring J.A., Nettleton P.F., Lowings J.P., Paton D.J. Pestiviruses isolated from pigs, cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis. Arch. Virol. 1994, 136:309-323.