Comparison of conventional versus real-time PCR detection of Brugia malayi DNA from dried blood spots from school children in a low endemic area

Tropical Biomedicine

Volumn 27, Issue 1, 2010, Pages 54-59

  Rahmah, N ^a   Nurulhasanah, O ^a   Norhayati, S ^b   Zulkarnain, I ^a   Norizan, M ^a  

^a SCHOOL OF PHYSICS   (Malaysia)

^b Vector borne Disease Control Unit   (Malaysia)

Author keywords

[No Author keywords available]

Indexed keywords

HELMINTH DNA;

ANIMAL; ARTICLE; BRUGIA MALAYI; CHILD; COMPARATIVE STUDY; ENDEMIC DISEASE; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METHODOLOGY; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

ANIMALS; BRUGIA MALAYI; CHILD; DNA, HELMINTH; ENDEMIC DISEASES; HUMANS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 77949633840     PISSN: 01275720     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (14)

1. Bockarie, M.J., Fischer, P., Williams, S.A., Zimmerman, P.A., Griffin, L., Alpers, M.P. & Kazura J.W. (2000). Application of a polymerase chain reaction-ELISA to detect Wuchereria bancrofti in pools of wild-caught Anopheles punctulatus in a filariasis control area in Papua New Guinea. American Journal of Tropical Medicine and Hygiene 62: 363-367.
2. Fischer, P., Boakye, D. & Hamburger, J. (2003). Polymerase chain reaction based detection of lymphatic filariasis. Medical Microbiology and Immunology 192: 3-7.
3. Fischer, P., Bonow, I., Supali, T., Rückert, P. & Rahmah, N. (2005). Detection of filarial-specific IgG4 antibodies and filarial DNA, for the screening of blood spots for Brugia timori. Annals of Tropical Medicine and Parasitology 99: 1-8.
4. Fischer, P., Wibowo, H., Pischeke, S., Rückert, P., Liebau, E., Ismid, I.S. & Supali, T. (2002). PCR-based detection and identification of the filarial parasite Brugia timori from Alor Island, Indonesia. Annals of Tropical Medicine and Parasitology 8: 809-821.
5. Ghedin, E., Wang, S., Foster, J.M. & Slatko, B.E. (2004). First sequenced genome of a parasitic nematode. Trends In Parasitology 20: 151-153.
6. Goodman, D.S., Orelus, J.N., Roberts, J.M., Lammie, P.J. & Streit, T.G. (2003). PCR and mosquito dissection as tools to monitor filarial infection levels following mass treatment. Filaria Journal 2: 11.
7. Helmy, H., Fischer, P., Farid, H.A., Farid, M., Bradley, H. & Ramzy, R.M. (2004). Test strip detection of Wuchereria bancrofti amplified DNA in wild-caught Culex pipiens and estimation of infection rate by a PoolScreen algorithm. Tropical Medicine and International Health 9: 158-163.
8. Kluber, S., Supali, T., Williams, S.A., Liebau, E. & Fischer, P. (2001). Rapid PCR-based detection of Brugia malayi DNA from blood spots by DNA detection test strips. Transactions Royal Society Tropical Medicine & Hgyiene 95: 169-170.
9. Lim, B.H., Rahmah, N., Afifi, S.A.B., Ramli, A. & Mehdi, R. (2001). Comparison of Brugia-Elisa and Thick Blood Smear examination in a prevalence study of brugian filariasis in Setiu, Terengganu, Malaysia. Medical Journal of Malaysia 56: 491-496.
10. Lizotte, M.R., Supali, T., Partono, F. & Williams, S.A. (1994). A polymerase chain reaction assay for detection of Brugia malayi in blood. American Journal of Tropical Medicine and Hygiene 51: 314-321.
11. Mishra, K., Raj, D.K., Rupenangshu, K.H., Hazra, K., Aditya, P.D. & Prakash, C.S. (2007). The development and evaluation of single step multiplex PCR method for simultaneous detection of Brugia malayi and Wuchereria bancrofti. Molecular and Cellular Probes 21: 355-362.
12. Ottensen, E.A., Duke, B.O.L., Karam, M. & Behbehani, K. (1997). Strategies and tools for the control/elimination of lymphatic filariasis. Bulletin World Health Organization 75: 491-503.
13. Rao, R.U., Weil, G.J., Fischer, K., Supali, T. & Fischer, P. (2006). Detection of brugia parasite DNA in human blood by real time PCR. Journal of Clinical Microbiology 44: 3887-3893.
14. Supali, T., Ismid, I.S., Wibowo, H., Djuardi, Y., Majawati, E., Ginanjar, P. & Fischer, P. (2006). Estimation of the prevalence of lymphatic filariasis by a pool screen PCR assay using blood spots collected on filter paper. Transactions Royal Society Tropical Medicine & Hygiene. 100: 753-759.