A metal-supported biomimetic micromembrane allowing the recording of single-channel activity and of impedance spectra of membrane proteins

Bioelectrochemistry

Volumn 78, Issue 2, 2010, Pages 176-180

  Becucci, Lucia ^a   D'Amico, Massimo ^a   Daniele, Salvatore ^b   Olivotto, Massimo ^a   Pozzi, Andrea ^a   Guidelli, Rolando ^a  

^a UNIVERSITY OF FLORENCE   (Italy)

^b CA' FOSCARI UNIVERSITY OF VENICE   (Italy)

Author keywords

Electrochemical impedance spectroscopy; OmpF porin; Single channel current; Tethered bilayer lipid membrane

Indexed keywords

ACTIVE STATE; BI-LAYER; BILAYER LIPID MEMBRANE; CHANNEL PROTEINS; DETERGENT SOLUTION; DRUG SCREENING; ELECTROCHEMICAL IMPEDANCE SPECTRA; HIGH THROUGHPUT; HYDROPHILIC SPACERS; IMPEDANCE SPECTRUM; ION TRANSLOCATION; LIPID MONOLAYER; MEMBRANE PROTEINS; MICROMEMBRANES; ON CURRENTS; PATCH CLAMP TECHNIQUE; PROTEIN MICROARRAY; SELF-ASSEMBLING; SINGLE-CHANNEL; SINGLE-CHANNEL CURRENT; STATISTICAL ANALYSIS;

AVIATION; BIOLOGICAL MEMBRANES; BIOMIMETICS; BIOPHYSICS; CELL MEMBRANES; ELECTROCHEMICAL CORROSION; ELECTRON ENERGY LEVELS; GRAPHIC METHODS; HYDROPHILICITY; LIPIDS; MERCURY (METAL); MONOLAYERS; PLATINUM; PROTEINS; SOLID SOLUTIONS; STATISTICAL METHODS;

ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY;

OUTER MEMBRANE PROTEIN F;

ARTICLE; BILAYER MEMBRANE; BIOMIMETICS; ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY; HISTOGRAM; HYDROPHILICITY; LIPID MEMBRANE; LIPID MONOLAYER; PATCH CLAMP; TETHERED BILAYER LIPID MICROMEMBRANE;

BIOMIMETICS; CALCIUM-BINDING PROTEINS; ELECTRODES; ETHER-A-GO-GO POTASSIUM CHANNELS; GRAMICIDIN; HYDROPHOBICITY; LIPID BILAYERS; MELITTEN; MEMBRANE MICRODOMAINS; MERCURY; MUSCLE PROTEINS; PATCH-CLAMP TECHNIQUES; PORINS; PROTEIN TRANSPORT; PROTEOLIPIDS;

EID: 77949571312     PISSN: 15675394     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bioelechem.2009.08.007     Document Type: Article

References (31)

1. Favero G., Campanella L., Cavallo S., D'Annibale A., Perrella M., Mattei E., and Ferri T. Glutamate receptor incorporated in a mixed hybrid bilayer lipid membrane array, as a sensing element of a biosensor working under flowing conditions. J. Am. Chem. Soc. 127 (2005) 8103-8111
2. Römer W., and Steinem C. Impedance analysis and single-channel recordings on nano-black lipid membranes based on porous alumina. Biophys. J. 86 (2004) 955-965
3. Guidelli R., Aloisi G., Becucci L., Dolfi A., Moncelli M.R., and Tadini Buoninsegni F. New directions and challenges in electrochemistry. Bioelectrochemistry at metal/water interfaces. J. Electroanal. Chem. 504 (2001) 1-28
4. Schiller S.M., Naumann R., Lovejoy K., Kunz H., and Knoll W. Archea analogue thiolipids for tethered bilayer lipid membranes on ultrasmooth gold surfaces. Angew. Chem. Int. Ed. 42 (2003) 208-211
5. + ion transport through tethered bilayer lipid membranes. J. Electroanal. Chem. 550 (2003) 241-252
6. He L., Robertson J.W.F., Li J., Kärcher I., Schiller S.M., Knoll W., and Naumann R. Tethered bilayer lipid membranes based on monolayers of thiolipids mixed with a complementary dilution molecule. 1. Incorporation of channel peptides. Langmuir 21 (2005) 11666-11672
7. Vitovic P., Kresák S., Naumann R., Schiller S.M., Lewis R.N.A.H., McElhaney R.N., and Hianik T. The study of the interaction of a model α-helical peptide with lipid bilayers and monolayers. Bioelectrochemistry 63 (2004) 169-176
8. Vockenroth I.K., Ohm C., Robertson J.W.F., McGillivray D.J., Lösche M., and Köper I. Stable insulating tethered bilayer lipid membranes. Biointerphases 3 (2008) FA68-FA73
9. J. Leitch, J. Kunze, J.D. Goddard, A.L. Schwan, R.J. Faragher, R. Naumann, W. Knoll, J.R. Dutcher, J. Lipkowski, In situ PM-IRRAS studies of an archea analogue thiolipid assembled on a Au(111) electrode surface, DOI: 10.1021/la900907d.
10. McGillivray D.J., Valincius G., Vanderah D.J., Febo-Ayala W., Woodward J.T., Heinrich F., Kasianowicz J.J., and Lösche M. Molecular-scale structural and functional characterization of sparsely tethered bilayer lipid membranes. Biointerphases 2 (2007) 21-33
11. Becucci L., Moncelli M.R., and Guidelli R. Impedance spectroscopy of OmpF porin reconstituted into a mercury-supported lipid bilayer. Langmuir 22 (2006) 1341-1346
12. Becucci L., Carbone M.V., Biagiotti T., D'Amico M., Olivotto M., and Guidelli R. Incorporation of the HERG potassium channel in a mercury supported lipid bilayer. J. Phys. Chem. B 112 (2008) 1315-1319
13. + ions in the hydrophilic spacer of tethered bilayer lipid membranes. Soft Matter 5 (2009) 2294-2301
14. Grahame D.C. Differential capacity on mercury in aqueous sodium fluoride solutions. I. Effect of concentration at 25. J. Am. Chem. Soc. 76 (1954) 4819-4823
15. Clavilier J., and Nguyen Van Huong C. Etude de la structure de la couche double sur les électrodes d'or. J. Electroanal. Chem. 41 (1973) 193-199
16. Becucci L., Moncelli M.R., Naumann R., and Guidelli R. Potassium ion transport by valinomycin across a Hg-supported lipid bilayer. J. Am. Chem. Soc. 127 (2005) 13316-13323
17. Becucci L., Romero León R., Moncelli M.R., Rovero P., and Guidelli R. Electrochemical investigation of melittin reconstituted into a mercury-supported lipid bilayer. Langmuir 22 (2006) 6644-6650
18. Becucci L., and Guidelli R. Kinetics of channel formation in bilayer lipid membranes (BLMs) and tethered BLMs: monazomycin and melittin. Langmuir 23 (2007) 5601-5608
19. Becucci L., Santucci A., and Guidelli R. Gramicidin conducting dimers in lipid bilayers are stabilized by single-file ionic flux along them. J. Phys. Chem. B 111 (2007) 9814-9820
20. Becucci L., Guidelli R., Karim C.B., Thomas D.D., and Veglia G. An electrochemical investigation of sarcolipin reconstituted into a mercury-supported lipid bilayer. Biophys. J. 93 (2007) 2678-2687
21. Becucci L., Cembran A., Karim C.B., Thomas D.D., Guidelli R., Gao J., and Veglia G. On the function of pentameric phospholamban: ion channel or storage form?. Biophys. J. 96 (2009) L60-L62
22. Daniele S., Ciani I., and Battistel D. Effect of the insulating shield thickness on the steady-state diffusion-limiting current of sphere cap microelectrodes. Anal. Chem. 80 (2008) 253-258
23. Becucci L., Moncelli M.R., and Guidelli R. Ion carriers and channels in metal-supported lipid bilayers as probes of transmenbrane and dipole potential. Langmuir 19 (2003) 3386-3392
24. Schindler H., and Rosenbusch J.P. Matrix protein from Escherichia coli outer membranes forms voltage-controlled channels in lipid bilayers. Proc. Natl. Acad. Sci. U. S. A. 75 (1978) 3751-3755
25. Nestorovich E.M., Danelon C., Winterhalter M., and Bezrukov S.M. Designed to penetrate: time-resolved interaction of single antibiotic molecules with bacterial pores. Proc. Natl. Acad. Sci. U. S. A. 99 (2002) 9789-9794
26. Iyer R., and Delcour A.H. Complex inhibition of OmpF and OmpC bacterial porins by polyamines. J. Biol. Chem. 272 (1997) 18595-18601
27. Belmont C., Tercier M.L., Buffle L., Fiaccabrino G.C., and Koudelka-Hep M. Mercury-plated iridium-based microelectrode arrays for trace metals detection by voltammetry: optimum conditions and reliability. Anal. Chim. Acta 329 (1996) 203-214
28. Silva R.M., El Khakani M.A., Chaker M., Champagne G.Y., Chevalet J., Gastonguay L., Lacasse R., and Ladouceur M. Development of Hg-electroplated iridium-based microelectrode arrays for heavy metal traces analysis. Anal. Chim. Acta 385 (1999) 249-255
29. Wang J., Wang J., Adeniyi W.K., and Kounaves S. Adsorptive stripping analysis of trace nickel at iridium-based ultramicroelectrode arrays. Electroanalysis 12 (2000) 44-47
30. Coldrick Z., Steenson P., Millner P., Davies M., and Nelson A. Phospholipid monolayer coated microfabricated electrodes to model the interaction of molecules with biomembranes. Electrochim. Acta 54 (2009) 4954-4962
31. A. Nelson, Z. Coldrick, PCT Application No. PCT/GB2008/002591:priority filing 31.07.07.