Realtime PCR is more sensitive than multiplex PCR for diagnosis and serotyping in children with culture negative pneumococcal invasive disease

PLoS ONE

Volumn 5, Issue 2, 2010, Pages

  Azzari, Chiara ^a   Moriondo, Maria ^a   Indolfi, Giuseppe ^a   Cortimiglia, Martina ^a   Canessa, Clementina ^a   Becciolini, Laura ^a   Lippi, Francesca ^a   De Martino, Maurizio ^a   Resti, Massimo ^a  

^a UNIVERSITY OF FLORENCE   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIUM CULTURE; BACTERIUM ISOLATE; BLOOD SAMPLING; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; HUMAN; INTERMETHOD COMPARISON; INVASIVE PNEUMOCOCCAL DISEASE; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NOSE SMEAR; PEDIATRIC HOSPITAL; PNEUMOCOCCAL INFECTION; REAL TIME POLYMERASE CHAIN REACTION; SEROTYPING; CHILD; CLASSIFICATION; COMPARATIVE STUDY; DIAGNOSTIC PROCEDURE; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; STREPTOCOCCUS PNEUMONIAE;

BACTERIA (MICROORGANISMS);

BACTERIAL DNA; BACTERIAL PROTEIN; CPSA PROTEIN, STREPTOCOCCUS PNEUMONIAE; PRIMER DNA;

BACTERIAL PROTEINS; CHILD; DNA PRIMERS; DNA, BACTERIAL; HUMANS; MOLECULAR DIAGNOSTIC TECHNIQUES; PNEUMOCOCCAL INFECTIONS; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SEROTYPING; STREPTOCOCCUS PNEUMONIAE;

EID: 77949511183     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0009282     Document Type: Article

References (39)

1. Bridy-Pappas AE, Margolis MB, Center KJ, Isaacman DJ (2005) Streptococcus pneumoniae: description of the pathogen, disease epidemiology, treatment, and prevention. Pharmacotherapy 25: 1193-1212.
2. World Health Organization (WHO) (2007) Pneumococcal conjugate vaccine for childhhood immunization-WHO position paper. Wkly Epidemiol Rec 82: 93-104.
3. Jin P, Kong F, Xiao M, Oftadeh S, Zhou F, et al. (2009) First Report of Putative Streptococcus pneumoniae Serotype 6D among Nasopharyngeal Isolates from Fijian Children. J Infect Dis 200(9): 1375-80.
4. Whitney CG, Farley MM, Hadler J, Harrison LH, Bennett NM, et al. (2003) Decline in invasive pneumococcal disease after the introduction of protein-polysaccharide conjugate vaccine. N Engl J Med 348: 1737-1746.
5. Arai S, Konda T, Wad A, Matsunaga Y, Okabe N, et al. (2001) Use of antiserum-coated latex particles for serotyping Streptococcus pneumoniae. Microbiol Immunol 45: 159-162.
6. Lalitha MK, Thomas K, Kumar RS, Steinhoff MC (1999) Serotyping of Streptococcus pneumoniae by coagglutination with 12 pooled antisera. J Clin Microbiol 37: 263-265.
7. Heidelberger M (1983) Precipitating cross-reactions among pneumococcal types. Infect Immun 41: 1234-1244.
8. Henrichsen J (1999) Typing of Streptococcus pneumoniae: past, present, and future. Am J Med 107: 50S-54S.
9. Kumar A, Mariappuram J, Kim CH (1985) Discrepancies in fluorescent antibody, counterimmunoelectrophoresis, and Neufeld test for typing of Streptococcus pneumoniae. Diagn Microbiol Infect Dis 3: 509-514.
10. Resti M, Micheli A, Moriondo M, Becciolini L, Cortimiglia M, et al. (2009) Comparison of the effect of antibiotic treatment on the possibility to diagnose invasive pneumococcal disease by cultural or molecular methods: a prospective observational study of children with proven pneumococcal infection. Clin Therapeutics 31: 1266-1273.
11. Charalambous BM, Oriyo NM, Gillespie SH (2008) How valid is single colony isolation for surveillance of Streptococcus pneumoniae carriage? J Clin Microbiol 46: 2467-468.
12. Azzari C, Resti M (2008) Reducing carriage and transmission: the beneficial "side effect" of pneumococcal conjugate vaccine. Clin Infect Dis 47: 997-999.
13. Corless CE, Guiver M, Borrow R, Edwards-Jones V, Fox AJ, et al. (2001) Simultaneous detection of Neisseria meningitidis, Haemophilus influenzae and Streptococcus pneumoniae in suspected cases of meningitis and septicemia using real-time PCR. J Clin Microbiol 39: 1553-1558.
14. Azzari C, Moriondo M, Indolfi G, Massai C, Becciolini L, et al. (2008) Molecular detection and serotyping on clinical samples improve diagnostic sensitivity and reveal increased incidence of invasive disease by Streptococcus pneumoniae in Italian children. J Med Microbiol 57: 1205-1212.
15. Saha SK, Darmstadt GL, Baqui AH, Hossain B, Islam M, et al. (2008) Identification of Serotype in Culture Negative Pneumococcal Meningitis Using Sequential Multiplex PCR: Implication for Surveillance and Vaccine Design. PLoS ONE 3: e3576.
16. Heid CA, Stevens J, Livak KJ, Williams PM (1996) Real time quantitative PCR. Genome Res 6: 986-994.
17. Mackay IM (2004) Real-time PCR in the microbiology laboratory. Clin Microbiol Infect 10: 190-212.
18. Carvalho MG, Tondella ML, McCaustland K, Weidlich L, McGee L, et al. (2007) Evaluation and improvement of real-time PCR assays targeting lyt, ply, and psaA genes for detection of pneumococcal DNA. J Clin Microbiol 45: 2460-2466.
19. Sandgren A, Sjostrom K, Olsson-Liljequist B, Christensson B, Samuelsson A, et al. (2004) Effect of clonal and serotype-specific properties on the invasive capacity of Streptococcus pneumoniae. J Infect Dis 189: 785-796.
20. Pai R, Gertz RE, Beall B (2006) Sequential multiplex PCR approach for determining capsular serotypes of Streptococcus pneumoniae isolates. J Clin Microbiol 44: 124-131.
21. Martell M, Gómez J, Esteban JI, Sauleda S, Quer J, et al. (1999) High-throughput real-time reverse transcription-PCR quantitation of hepatitis C virus RNA. J Clin Microbiol 37: 327-332.
22. Lallemand F, Desire N, Rozenbaum W, Nicolas JC, Marechal V (2000) Quantitative analysis of human herpesvirus 8 viral load using a real-time PCR assay. J Clin Microbiol 38: 1404-1408.
23. Hare KM, Morris P, Smith-Vaughan H, Leach AJ (2008) Random colony selection versus colony morphology for detection of multiple pneumococcal serotypes in nasopharyngeal swabs. Pediatr Infect Dis J 27: 178-0.
24. Brugger SD, Hathaway LJ, Muhlemann K (2009) Detection of Streptococcus pneumoniae Strain Cocolonization in the Nasopharynx. J Clin Microbiol 47: 1750-56.
25. Vestrheim DF, Høiby EA, Aaberge IS, Caugant DA (2008) Phenotypic and Genotypic Characterization of Streptococcus pneumoniae Strains Colonizing Children Attending Day-Care Centers in Norway. J Clin Microbiol 46: 2508-18.
26. Tarallo L, Tancredi F, Schito G, Marchese A, Bella A (2006) Italian Pneumonet Group (Società Italiana Pediatria and Associazione Italiana Studio Antimicrobici e Resistenze) Active surveillance of Streptococcus pneumoniae bacteremia in Italian children. Vaccine 24: 6938-6943.
27. Isaacman DJ, McIntosh ED, Reinert RR (2009) Burden of invasive pneumococcal disease and serotype distribution among Streptococcus pneumoniae isolates in young children in Europe: impact of the 7-valent pneumococcal conjugate vaccine and considerations for future conjugate vaccines. Int J Infect Dis Aug 21: [Epub ahead of print].
28. Center for Disease Control and Prevention (CDC) (2008) Invasive pneumococcal disease in children 5 years after conjugate vaccine introduction-eight states, 1998-2005. MMWR 57: 144-148.
29. Murdoch DR, Anderson TP, Beynon KA, Chua A, Fleming AM, et al. (2003) Evaluation of a PCR assay fro detection of Streptococcus pneumoniae in respiratory and nonrespiratory samples from adults with community-acquired pneumonia. J Clin Microbiol 41: 63-66.
30. Dagan R, Shriker O, Hazan I, Leibovitz E, Greenberg D, et al. (1998) Prospective study to determine clinical relevance of detection of pneumococcal DNA in sera of children by PCR. J Clin Microbiol 36: 669-673.
31. Hausdorff WP, Sieber G, Paradiso PR (2001) Geographical differences in invasive pneumococcal disease rates and serotype frequency in young children. Lancet 357: 950-952.
32. Saha SK, Naheed A, El Arifeen S, Islam M, Al-Emran H, et al. (2009) Surveillance for invasive Streptococcus pneumoniae disease among hospitalised children in Bangladesh: antimicrobial susceptibility and serotypedistribution. Clin Infect Dis 48 Suppl 2: S75-81.
33. Byington CL, Samore MH, Stoddard GJ, Barlow S, Daly J, et al. (2005) Temporal Trends of Invasive Disease Due to Streptococcus pneumoniae among Children in the Intermountain West: Emergence of Nonvaccine Serogroups. Clin Infect Dis 41: 21-29.
34. Finland M, Barnes MW (1977) Changes in Occurrence of Capsular Serotypes of Streptococcus pneumoniae at Boston City Hospital During Selected Years Between 1935 and 1974. J Clin Microbiol 5: 154-156.
35. Butler JC, Breiman RF, Lipman HB, Hofmann J, Facklam RR (1995) Serotype distribution of Streptococcus pneumoniae infections among preschool children in the United States 1978-1994: implications for development of a conjugate vaccine. J Infect Dis 171: 885-889.
36. Tarragó D, Fenoll A, Sánchez-Tatay D, Arroyo LA, Muñoz-Almagro C, et al. (2008) Identification of pneumococcal serotypes from culture-negative clinical specimens by novel real-time PCR. Clin Microbiol Infect 14: 828-834.
37. Pai R, Limor J, Beall B (2005) Use of Pyrosequencing To Differentiate Streptococcus pneumoniae Serotypes 6A and 6B. J Clin Microbiol 43: 4820-4822.
38. Pimenta FC, Gertz RE, Roundtree A, Yu J, Nahm MH, et al. (2009) Rarely Occurring 19A-Like cps Locus from a Serotype 19F Pneumococcal Isolate Indicates Continued Need of Serology-Based Quality Control for PCR-Based Serotype Determinations. J Clin Microbiol 47: 2353-2354.
39. Hsu HE, Shutt KA, Moore MR, Beall BW, Bennett NM, et al. (2009) Effect of pneumococcal conjugate vaccine on pneumococcal meningitis. N Engl J Med 360: 244-246.