An MHC-I cytoplasmic domain/HIV-1 nef fusion protein binds directly to the μ subunit of the AP-1 endosomal coat complex

PLoS ONE

Volumn 4, Issue 12, 2009, Pages

  Singh, Rajendra Kumar ^a   Lau, David ^a   Noviello, Colleen M ^a   Ghosh, Partho ^b   Guatelli, John C ^a,c  

^a UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c VA SAN DIEGO HEALTHCARE SYSTEM   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; GLUTAMINE; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 1; NEF PROTEIN; RECOMBINANT PROTEIN; SERINE; TRANSCRIPTION FACTOR AP 1; TYROSINE;

AMINO ACID SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; CYTOPLASM; DOWN REGULATION; ENDOSOME; HUMAN IMMUNODEFICIENCY VIRUS 1; HYDROPHOBICITY; NONHUMAN; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN MOTIF; PROTEIN PROTEIN INTERACTION;

ADAPTOR PROTEIN COMPLEX MU SUBUNITS; AMINO ACIDS; CD8-POSITIVE T-LYMPHOCYTES; CELL LINE; CELL MEMBRANE; CYTOPLASM; DOWN-REGULATION; ENDOSOMES; HISTOCOMPATIBILITY ANTIGENS CLASS I; HUMANS; NEF GENE PRODUCTS, HUMAN IMMUNODEFICIENCY VIRUS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS;

HUMAN IMMUNODEFICIENCY VIRUS 1; PRIMATE LENTIVIRUS GROUP;

EID: 77949496852     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0008364     Document Type: Article

References (22)

1. Collins KL, Chen BK, Kalams SA, Walker BD, Baltimore D (1998) HIV-1 Nef protein protects infected primary cells against killing by cytotoxic T lymphocytes. Nature 391: 397-401.
2. Benson RE, Sanfridson A, Ottinger JS, Doyle C, Cullen BR (1993) Downregulation of cell-surface CD4 expression by simian immunodeficiency virus Nef prevents viral super infection. J Exp Med 177: 1561-1566.
3. Garcia JV, Miller AD (1991) Serine phosphorylation-independent downregula- tion of cell-surface CD4 by nef. Nature 350: 508-511.
4. Aiken C, Konner J, Landau NR, Lenburg ME, Trono D (1994) Nef induces CD4 endocytosis: requirement for a critical dileucine motif in the membrane- proximal CD4 cytoplasmic domain. Cell 76: 853-864.
5. Le Gall S, Erdtmann L, Benichou S, Torrent CB, Liu L, et al. (1998) Nef interacts with the μ subunit of clathrin adaptor complexes and reveals a cryptic sorting signal in MHC 1 molecules. Immunity 8: 483-495.
6. Schaefer MR, Wonderlich ER, Roeth JF, Leonard JA, Collins KL (2008) HIV-1 Nef targets MHC-I and CD4 for degradation via a final common b-COP- dependent pathway in T cells. PLoS Pathog 22:4(8): e1000131.
7. Baur AS, Sawai ET, Dazin P, Fantl WJ, Cheng-Mayer C, et al. (1994) HIV-1 Nef leads to inhibition or activation of T cells depending on its intracellular localization. Immunity 5: 373-384.
8. Kaminchik J, Bashan N, Itach A, Sarver N, Gorecki M (1991) Genetic characterization of human immunodeficiency virus type 1 nef gene products translated in vitro and expressed in mammalian cells. J Virol 65:583-588.
9. Robinson M S (2004) Adaptable adaptors for coated vesicles. Trends Cell Biol 4: 167-174.
10. Roeth JF, Williams M, Kasper MR, Filzen TM, Collins KL (2004) HIV-1 Nef disrupts MHC-I trafficking by recruiting AP-1 to the MHC-I cytoplasmic tail. J Cell Biol 167: 903-913.
11. Owen DJ, Evans PR (1998) A structural explanation for the recognition of tyrosine-based endocytotic signals. Science 282: 1327-1332.
12. Kelly BT, McCoy AJ, Späte K, Miller SE, Evans PR, et al. (2008) A structural explanation for the binding of endocytic dileucine motifs by the AP2 complex. Nature 456: 976-979.
13. Chaudhuri R, Mattera R, Lindwasser OW, Robinson MS, Bonifacino JS (2009) A basic patch on alpha-adaptin is required for binding of human immunode- ficiency virus type 1 Nef and cooperative assembly of a CD4-Nef-AP-2 complex. J Virol 83: 2518-2530.
14. Noviello CM, Benichou S, Guatelli JC (2008) Cooperative binding of the class I major histocompatibility complex cytoplasmic domain and human immunode- ficiency virus type 1 Nef to the endosomal AP-1 complex via its μ subunit. J Virol 82: 1249-1258.
15. Geyer M, Munte CE, Schorr J, Kellner R, Kalbitzer HR (1999) Structure of the anchor-domain of myristoylated and non-myristoylated HIV-1 Nef protein. J Mol Biol 289: 123-138.
16. Grzesiek S, Bax A, Hu JS, Kaufman J, Palmer I, et al. (1997) Refined solution structure and backbone dynamics of HIV-1 Nef. Protein Sci 6: 1248-1263.
17. Coleman SH, Van Damme N, Day JR, Noviello CM, Hitchin D, et al. (2005) Leucine-specific, functional interactions between human immunodeficiency virus type 1 Nef and adaptor protein complexes. J Virol. 79: 2066-2078.
18. Janvier K, Kato Y, Boehm M, Rose JR, Martina JA, et al. (2003) Recognition of dileucine-based sorting signals from HIV-1 Nef and LIMP-II by the AP-1 gamma-sigma1 and AP-3 delta-sigma3 hemicomplexes. J Cell Biol. 163: 1281-1290.
19. Riggs NL, Craig HM, Pandori MW, Guatelli J (1999) The dileucine-based sorting motif in HIV-1 nef is not required for down-regulation of class I MHC. Virology 258: 203-207.
20. Wonderlich ER, Williams M, Collins KL (2008) The tyrosine binding pocket in the adaptor protein 1 (AP-1) m1 subunit is necessary for Nef to recruit AP-1 to the major histocompatibility complex class I cytoplasmic tail. J Biol Chem 283: 3011-3022.
21. Piguet V, Wan L, Borel C, Mangasarian A, Demaurex N, et al. (2000) HIV-1 Nef protein binds to the cellular protein PACS-1 to downregulate class I major histocompatibility complexes. Nature Cell Biology 2: 163-167.
22. Heldwein EE, Macia E, Wang J, Yin HL, Kirchhausen T, et al. (2004) Crystal structure of the clathrin adaptor protein 1 core. PNAS 101: 14108-14113.