Development of in situ hybridization assay that differentiates between two genotypes of Porcine circovirus-2 in formalin-fixed, paraffin-embedded tissues

Journal of Veterinary Diagnostic Investigation

Volumn 22, Issue 2, 2010, Pages 231-233

  Kim, Duyeol ^a   Ha, Yooncheol ^a   Oh, Yeonsu ^a   Han, Kiwon ^a   Noh, Seung Ho ^b   Kim, Cheolwoo ^c   Kim, Sung Hoon ^c   Chae, Chanhee ^a  

^a Seoul National University   (South Korea)

^b Hanyang University   (South Korea)

^c Kyung Hee University   (South Korea)

Author keywords

Formalin fixed tissue; Genotype; In situ hybridization; Porcine circovirus 2

Indexed keywords

PORCINE CIRCOVIRUS 2; SUIDAE;

EID: 77949337574     PISSN: 10406387     EISSN: None     Source Type: Journal    
DOI: 10.1177/104063871002200209     Document Type: Article

References (10)

1. Calsamiglia M, Segales J, Quintana J, et al.: 2002, Detection of porcine circovirus types 1 and 2 in serum and tissue samples of pigs with and without postweaning multisystemic wasting syndrome. J Clin Microbiol 40:1848-1850.
2. Chae C: 2004, Postweaning multisystemic wasting syndrome: a review of aetiology, diagnosis and pathology. Vet J 168:41-49.
3. Chae C: 2005, A review of porcine circovirus 2-associated syndromes and disease. Vet J 169:326-336.
4. Gagnon CA, Tremblay D, Tijssen P, et al.: 2007, The emergence of porcine circovirus 2b genotype (PCV-2b) in swine in Canada. Can Vet J 48:811-819.
5. Grau-Roma L, Crisci E, Sibila M, et al.: 2008, A proposal on porcine circovirus type 2 (PCV2) genotype definition and their relation with postweaning multisystemic wasting syndrome (PMWS) occurrence. Vet Microbiol 128:23-35.
6. Kim J, Choi C, Chan C: 2003, Pathogenesis of postweaning multisystemic wasting syndrome reproduced by co-infection with Korean isolates of porcine circovirus 2 and porcine parvovirus. J Comp Pathol 128:52-59.
7. Lyoo KS, Kim HB, Joo HS: 2008, Evaluation of a nested polymerase chain reaction assay to differentiate between two genotypesofPorcinecircovirus-2. J Vet Diagn Invest 20:283-288.
8. Mankertz A, Persson F, Mankertz J, et al.: 1997, Mapping and characterization of the origin of DNA replication of porcine circovirus. J Virol 71:2562-2566.
9. Meehan BM, McNeilly F, Todd D, et al.: 1998, Characterization of novel circovirus DNAs associated with wasting syndromes in pigs. J Gen Virol 79:2171-2179.
10. Segales J, Olvera A, Grau-Roma L, et al.: 2008, PCV-2 genotype definition and nomenclature. Vet Rec 162:867-868.