Optimization of a whole blood gamma interferon assay for the detection of sheep infected with mycobacterium avium subspecies paratuberculosis

Journal of Veterinary Diagnostic Investigation

Volumn 22, Issue 2, 2010, Pages 210-217

  Bosward, Katrina L ^a   Dhand, Navneet K ^a   Begg, Douglas J ^a   Thomson, Peter C ^a   Emery, David L ^a   Whittington, Richard J ^a  

^a UNIVERSITY OF SYDNEY   (Australia)

Author keywords

Bovigam assay; Gamma interferon; Johne's disease; Paratuberculosis; Sheep

Indexed keywords

ANIMALIA; BACTERIA (MICROORGANISMS); BOS; MYCOBACTERIUM AVIUM; OVIS ARIES;

EID: 77949335054     PISSN: 10406387     EISSN: None     Source Type: Journal    
DOI: 10.1177/104063871002200206     Document Type: Article

References (15)

1. Bassey EOE, Collins MT: 1997, Study of T-lymphocyte subsets of healthy and Mycobacterium avium subsp. paratuberculosis-infected cattle. Infect Immun 65:4869-4872.
2. Bilman-Jacobe H, Carrigan M, Cockram F, et al.: 1992, A comparison of the interferon gamma assay with the absorbed ELISA for the diagnosis of Johne's disease in cattle. Aust Vet J 69:25-28.
3. Burrells C, Clarke CJ, Colston A, et al.: 1999, Interferongamma and interleukin 2 release by lymphocytes derived from the blood, mesenteric lymph nodes and intestines of normal sheep and those with paratuberculosis (Johne's disease). Vet Immunol Immunopathol 68:139-148.
4. Cehovin A, Cliff JM, Hill PC, et al.: 2007, Extended culture enhances sensitivity of a gamma interferon assay for latent Mycobacterium tuberculosis infection. Clin Vaccine Immunol 14:796-798.
5. Elsasser-Beile U, Von Kleist S, Gallati H: 1991, Evaluation of a test system for measuring cytokine production in human whole blood cell cultures. J Immunol Methods 139:191-195.
6. Emery DL, Rothel JS, Wood PR: 1990, Influence of antigens and adjuvants on the production of gamma-interferon and antibody by ovine lymphocytes. Immunol Cell Biol 68:127-136.
7. Gwozdz JM, Thompson KG: 2002, Antigen-induced production of interferon-c in samples of peripheral lymph nodes from sheep experimentally inoculated with Mycobacterium avium subsp. paratuberculosis. Vet Microbiol 84:243-252.
8. Hanekom WA, Hughes J, Mavinkurve M, et al.: 2004, Novel application of a whole blood intracellular cytokine detection assay to quantitate specific T-cell frequency field studies. J Immunol Methods 291:185-195.
9. Jungersen G, Huda A, Hansen JJ, Lind P: 2002, Interpretation of the gamma interferon test for diagnosis of subclinical paratuberculosis in cattle. Clin Diagn Lab Immunol 9:453-460.
10. Pauly JL, Sokal JE, Han T: 1973, Whole-blood culture technique for functional studies of lymphocyte reactivity to mitogens, antigens, and homologous lymphocytes. J Lab Clin Med 82:500-512.
11. Robbe-Austerman S, Krull AC, Stabel JR: 2006, Time delay, temperature effects and assessment of positive controls on whole blood for the gamma interferon ELISA to detect paratuberculosis. J Vet Med B Infect Dis Vet Public Health 53:213-217.
12. Rothel JS, Jones SL, Corner LA, et al.: 1990, A sandwich enzyme immunoassay for bovine interferon-c and its use for the detection of tuberculosis in cattle. Aust Vet J 67:134-137.
13. Rothel JS, Jones SL, Corner LA, et al.: 1992, The gammainterferon assay for diagnosis of bovine tuberculosis in cattle: conditions affecting the production of gamma-interferon in whole blood culture. Aust Vet J 69:1-4.
14. Stabel JR, Whitlock RH: 2001, An evaluation of a modified interferon-c assay for the detection of paratuberculosis in dairy herds. Vet Immunol Immunopathol 79:69-81.
15. Stewart DJ, Vaughan JA, Stiles PL, et al.: 2004, A long-term study in Merino sheep experimentally infected with Mycobacterium avium subsp. paratuberculosis: clinical disease, faecal culture and immunological studies. Vet Microbiol 104:165-178.