Straightforward histochemical staining of Fe by the adaptation of an old-school technique: Identification of the endodermal vacuole as the site of fe storage in Arabidopsis embryos

Plant Signaling and Behavior

Volumn 5, Issue 1, 2010, Pages 56-57

  Roschzttardtz, Hannetz ^a   Conéjéro, Geneviève ^a   Curie, Catherine ^a   Mari, Stéphane ^a  

^a MONTPELLIER SUPAGRO   (France)

Author keywords

Arabidopsis; Embryo; Endodermis; Iron; Vacuole

Indexed keywords

IRON;

ARABIDOPSIS; ARTICLE; CELL VACUOLE; CYTOCHEMISTRY; METABOLISM; METHODOLOGY; PLANT MORPHOLOGY; PLANT SEED; PRENATAL DEVELOPMENT; STAINING; ULTRASTRUCTURE;

ARABIDOPSIS; HISTOCYTOCHEMISTRY; IRON; PLANT STRUCTURES; SEEDS; STAINING AND LABELING; VACUOLES;

ARABIDOPSIS;

EID: 77949293699     PISSN: 15592316     EISSN: 15592324     Source Type: Journal    
DOI: 10.4161/psb.5.1.10159     Document Type: Article

References (6)

1. Lanquar V, Lelievre F, Bolte S, Hames C, Alcon C, Neumann D, et al. Mobilization of vacuolar iron by AtNRAMP3 and AtNRAMP4 is essential for seed germination on low iron. EMBO J 2005; 24:4041-51.
2. Kim SA, Punshon T, Lanzirotti A, Li L, Alonso JM, Ecker JR, et al. Localization of iron in Arabidopsis seed requires the vacuolar membrane transporter VIT1. Science 2006; 314:1295-8.
3. Nguyen-Legros J, Bizot J, Bolesse M, Pulicani JP. "Diaminobenzidine black" as a new histochemical demonstration of exogenous iron (author's transl). Histochemistry 1980; 66:239-44.
4. Roschzttardtz H, Conéjéro G, Curie C, Mari S. Identification of the endodermal vacuole as the iron storage compartment in the Arabidopsis embryo. Plant Physiol 2009; 151:1329-38.
5. Scheres B, Dilaurenzio L, Willemsen V, Hauser MT, Janmaat K, Weisbeek P, Benfey PN. Mutations affecting the radial organization of the Arabidopsis root display specific defects throughout the embryonic axis. Development 1995; 121:53-62.
6. Deyholos MK, Cordner G, Beebe D, Sieburth LE. The SCARFACE gene is required for cotyledon and leaf vein patterning. Development 2000; 127:3205-13.