A protocol to effectively create single cell suspensions of adherent cells for multiparameter high-throughput flow cytometry

In Vitro Cellular and Developmental Biology - Animal

Volumn 46, Issue 2, 2010, Pages 97-101

  Glazer, Evan S ^a   Massey, Katheryn L ^a   Curley, Steven A ^a,b  

^a UNIVERSITY OF TEXAS MD ANDERSON CANCER CENTER   (United States)

^b Rice University   (United States)

Author keywords

Collagenase; FACS; High throughput flow cytometry; Multiparameter flow cytometry

Indexed keywords

CELL ADHESION MOLECULE; COLLAGENASE; DOXORUBICIN; TRYPSIN;

ARTICLE; CELL ADHESION; CELL CYCLE; CULTURE TECHNIQUE; DRUG EFFECT; FLOW CYTOMETRY; HUMAN; METABOLISM; METHODOLOGY; PANCREAS TUMOR; PATHOLOGY; TUMOR CELL LINE;

CELL ADHESION; CELL ADHESION MOLECULES; CELL CULTURE TECHNIQUES; CELL CYCLE; CELL LINE, TUMOR; COLLAGENASES; DOXORUBICIN; FLOW CYTOMETRY; HUMANS; PANCREATIC NEOPLASMS; TRYPSIN;

EID: 77949265986     PISSN: 10712690     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11626-009-9256-8     Document Type: Article

References (22)

1. T Araki M Yamada 1985 Fluorescence decay measurements for determining the relative content of ethidium bromide to DNA in situ in cell nuclei Histochemistry. 83 299 301 10.1007/BF00684374 1:CAS:528:DyaL2MXmtFShur8%3D 4066410
2. CF Brunk L Simpson 1977 Comparison of various ultraviolet sources for fluorescent detection of ethidium bromide-DNA complexes in polyacrylamide gels Anal Biochem. 82 455 462 10.1016/0003-2697(77)90183-X 1:CAS:528: DyaE2sXlsVyiu74%3D 907145
3. V Buschmann KD Weston M Sauer 2003 Spectroscopic study and evaluation of red-absorbing fluorescent dyes Bioconjug Chem. 14 195 204 10.1021/bc025600x 1:CAS:528:DC%2BD38XovFyktbw%3D 12526709
4. SM Cruickshank J Southgate PJ Selby LK Trejdosiewicz 1998 Expression and cytokine regulation of immune recognition elements by normal human biliary epithelial and established liver cell lines in vitro J. Hepatol. 29 550 558 10.1016/S0168-8278(98)80149-9 1:CAS:528:DyaK1cXnt1KktLo%3D 9824263
5. HH Cui JG Valdez JA Steinkamp HA Crissman 2003 Fluorescence lifetime-based discrimination and quantification of cellular DNA and RNA with phase-sensitive flow cytometry Cytometry A. 52 46 55 10.1002/cyto.a.10022 12596251
6. HJBH Davis MC Bene, et al. 2007 Bethesda International Consensus recommendations on the flow cytometric immunophenotypic analysis of hematolymphoid neoplasia: medical indications Cytometry B Clin Cytom. 72 S5 13 17803188
7. E Fiore C Fusco P Romero I Stamenkovic 2002 Matrix metalloproteinase 9 (MMP-9/gelatinase B) proteolytically cleaves ICAM-1 and participates in tumor cell resistance to natural killer cell-mediated cytotoxicity Oncogene. 21 5213 5223 10.1038/sj.onc.1205684 1:CAS:528:DC%2BD38Xls1Cltrs%3D 12149643
8. JE Gervasoni Jr AA Hindenburg MP Vezeridis S Schulze HJ Wanebo S Mehta 2004 An effective in vitro antitumor response against human pancreatic carcinoma with paclitaxel and daunorubicin by induction of both necrosis and apoptosis Anticancer Res. 24 2617 2626 1:CAS:528:DC%2BD2cXhtVSjsLzK 15517865
9. SF Ibrahim G van den Engh 2003 High-speed cell sorting: fundamentals and recent advances Curr Opin Biotechnol. 14 5 12 10.1016/S0958-1669(02)00009-5 1:CAS:528:DC%2BD3sXps1WitA%3D%3D 12565996
10. L Lamoreaux M Roederer R Koup 2006 Intracellular cytokine optimization and standard operating procedure Nature Protocols. 1 1507 1516 10.1038/nprot.2006.268 1:CAS:528:DC%2BD28Xht1eitL%2FP 17406442
11. JM Llovet J Bruix 2003 Systematic review of randomized trials for unresectable hepatocellular carcinoma: Chemoembolization improves survival Hepatology. 37 429 442 10.1053/jhep.2003.50047 1:CAS:528:DC%2BD3sXhsFeltrg%3D 12540794
12. G Marx H Zhou DE Graves N Osheroff 1997 Covalent attachment of ethidium to DNA results in enhanced topoisomerase II-mediated DNA cleavage Biochemistry. 36 15884 15891 10.1021/bi971858c 1:CAS:528:DyaK1cXmsVSl 9398321
13. S Matsuno H Hisano M Kobari S Akaishi 1990 Growth-inhibitory effect of combination chemotherapy for human pancreatic cancer cell lines Cancer. 66 2369 2374 10.1002/1097-0142(19901201)66:11<2369::AID-CNCR2820661120>3.0.CO;2-Z 1:CAS:528:DyaK3MXhvF2itb0%3D 2123126
14. PA McCourt B Ek N Forsberg S Gustafson 1994 Intercellular adhesion molecule-1 is a cell surface receptor for hyaluronan J. Biol. Chem. 269 30081 30084 1:CAS:528:DyaK2cXmslOnurs%3D 7527024
15. E Ohuchi K Imai Y Fujii H Sato M Seiki Y Okada 1997 Membrane type 1 matrix metalloproteinase digests interstitial collagens and other extracellular matrix macromolecules J. Biol. Chem. 272 2446 2451 10.1074/jbc.272.4.2446 1:CAS:528:DyaK2sXotFeguw%3D%3D 8999957
16. Phelan MC. Techniques for mammalian cell tissue culture. Curr Protoc Cytom. Appendix 3: Appendix 3B; 2006.
17. BL Sailer AJ Nastasi JG Valdez JA Steinkamp HA Crissman 1997 Differential effects of deuterium oxide on the fluorescence lifetimes and intensities of dyes with different modes of binding to DNA J. Histochem. Cytochem. 45 165 175 1:CAS:528:DyaK2sXhvVyls7o%3D 9016307
18. I Schmid J Ferbas CH Uittenbogaart JV Giorgi 1999 Flow cytometric analysis of live cell proliferation and phenotype in populations with low viability Cytometry. 35 64 74 10.1002/(SICI)1097-0320(19990101)35:1<64::AID- CYTO9>3.0.CO;2-Y 1:STN:280:DC%2BD3c%2FhvFKisg%3D%3D 10554182
19. S Shimoyama F Gansauge S Gansauge M Kaminishi HG Beger 1999 Basal expression and cytokine induction of intercellular adhesion molecule-1 in human pancreatic cancer cell lines J. Exp. Clin. Cancer Res. 18 107 110 1:STN:280:DyaK1MzgtFahtw%3D%3D 10374689
20. SD Sithu WR English P Olson, et al. 2007 Membrane-type 1-matrix metalloproteinase regulates intracellular adhesion molecule-1 (ICAM-1)-mediated monocyte transmigration J. Biol. Chem. 282 25010 25019 10.1074/jbc.M611273200 1:CAS:528:DC%2BD2sXpt1SltL4%3D 17591781
21. DM Vail MA Amantea GT Colbern FJ Martin RA Hilger PK Working 2004 Pegylated liposomal doxorubicin: proof of principle using preclinical animal models and pharmacokinetic studies Semin Oncol. 31 16 35 10.1053/j.seminoncol. 2004.08.002 1:CAS:528:DC%2BD2MXhtVyns7k%3D 15717736
22. RM Watt EW Voss Jr 1979 Solvent perturbation of the fluorescence of fluorescein bound to specific antibody. Fluorescence quenching of the bound fluorophore by iodide J Biol Chem. 254 1684 1690 1:CAS:528:DyaE1MXhs1Kgtbc%3D 570194