Construction of a femtosecond laser microsurgery system

Nature Protocols

Volumn 5, Issue 3, 2010, Pages 395-407

  Steinmeyer, Joseph D ^a   Gilleland, Cody L ^a   Pardo Martin, Carlos ^a,b   Angel, Matthew ^a   Rohde, Christopher B ^a   Scott, Mark A ^a,b   Yanik, Mehmet Fatih ^a  

^a Department of Electrical Engineering and Computer Science   (United States)

^b HARVARD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CAENORHABDITIS ELEGANS; CONTROLLED STUDY; FLUORESCENCE IMAGING; FLUORESCENCE MICROSCOPE; IMAGE ANALYSIS; IMAGING SYSTEM; LASER SURGERY; MICROSURGERY; NERVE FIBER TRANSECTION; NONHUMAN; OPTICS; PRIORITY JOURNAL;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; AXOTOMY; CAENORHABDITIS ELEGANS; EQUIPMENT DESIGN; LASER THERAPY; MICROSCOPY, FLUORESCENCE; MICROSURGERY; NERVE REGENERATION;

CAENORHABDITIS ELEGANS;

EID: 77749333462     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2010.4     Document Type: Article

References (29)

1. Vogel, A., Noack, J., Huttman, G. & Paltauf, G. Mechanisms of femtosecond laser nanosurgery of cells and tissues. Appl. Phys. B 81, 1015-1047 (2005).
2. Botvinick, E.L., Venugopalan, V., Shah, J.V., Liaw, L.H. & Berns, M.W. Controlled ablation of microtubules using a picosecond laser. Biophys. J. 87, 4203-4212 (2004).
3. Watanabe, W. et al. Femtosecond laser disruption of subcellular organelles in a living cell. Opt. Express 12, 4203-4213 (2004).
4. Heisterkamp, A. et al. Pulse energy dependence of subcellular dissection by femtosecond laser pulses. Opt. Express 13, 3690-3696 (2005).
5. Tirlapur, U.K. & König, K. Targeted transfection by femtosecond laser. Nature 418, 290-291 (2002).
6. Vogel, A. et al. Mechanisms of laser-induced dissection and transport of histologic specimens. Biophys. J. 93, 4481-4500 (2007).
7. Shen, N. et al. Ablation of cytoskeletal filaments and mitochondria in live cells using femtosecond laser nanoscissor. Mech. Chem. Biosyst. 2, 17-25 (2005).
8. Yanik, M.F. et al. Neurosurgery-functional regeneration after laser axotomy. Nature 432, 822 (2004).
9. Chung, S.H., Clark, D.A., Gabel, C.V., Mazur, E. & Samuel, A.D.T. The role of the AFD neuron in C. elegans thermotaxis analyzed using femtosecond laser ablation. BMC Neurosci. 7 (2006).
10. Yanik, M.F. et al. Nerve regeneration in Caenorhabditis elegans after femtosecond laser axotomy. IEEE J. Sel. Top. Quantum Electron. 12, 1283-1291 (2006).
11. Wu, Z. et al. Caenorhabditis elegans neuronal regeneration is infuenced by life stage, ephrin signaling, and synaptic branching. Proc. Natl. Acad. Sci. USA 104, 15132-15137 (2007).
12. Biron, D., Wasserman, S., Thomas, J.H., Samuel, A.D.T. & Sengupta, P. An olfactory neuron responds stochastically to temperature and modulates Caenorhabditis elegans thermotactic behavior. Proc. Natl. Acad. Sci. USA 105, 11002-11007 (2008).
13. Zhang, M. et al. A self-regulating feed-forward circuit controlling C. elegans egg-laying behavior. Curr. Biol. 18, 1445-1455 (2008).
14. Hammarlund, M., Nix, P., Hauth, L., Jorgensen, E.M. & Bastiani, M. Axon regeneration requires a conserved MAP kinase pathway. Science 323, 802-806 (2009).
15. Gabel, C.V., Antoine, F., Chuang, C.F., Samuel, A.D.T. & Chang, C. Distinct cellular and molecular mechanisms mediate initial axon development and adult-stage axon regeneration in C. elegans. Development 135, 3623-3623 (2008).
16. Chung, S.H. & Mazur, E. Femtosecond laser ablation of neurons in C. elegans for behavioral studies. Appl. Phys. A 96, 335-341 (2009).
17. Supatto, W. et al. In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses. Proc. Natl. Acad. Sci. USA 102, 1047-1052 (2005).
18. Nishimura, N. et al. Targeted insult to subsurface cortical blood vessels using ultrashort laser pulses: three models of stroke. Nat. Methods 3, 99-108 (2006).
19. Rohde, C.B., Zeng, F., Gonzalez-Rubio, R., Angel, M. & Yanik, M.F. Microfluidic system for on-chip high-throughput whole-animal sorting and screening at subcellular resolution. Proc. Natl. Acad. Sci. USA 104, 13891-13895 (2007).
20. Zeng, F., Rohde, C.B. & Yanik, M.F. Sub-cellular precision on-chip small-animal immobilization, multi-photon imaging and femtosecond-laser manipulation. Lab Chip 8, 653-656 (2008).
21. Guo, S.X. et al. Femtosecond laser nanoaxotomy lab-on-a chip for in vivo nerve regeneration studies. Nat. Methods 5, 531-533 (2008).
22. Hulme, S.E., Shevkoplyas, S.S., Apfeld, J., Fontana, W. & Whitesides, G.M. A microfabricated array of clamps for immobilizing and imaging C. elegans. Lab Chip 7, 1515-1523 (2007).
23. Chung, K.H., Crane, M.M. & Lu, H. Automated on-chip rapid microscopy, phenotyping and sorting of C. elegans. Nat. Methods 5, 637-643 (2008).
24. Colombelli, J., Reynaud, E.G. & Stelzer, E.H.K. Subcellular nanosurgery with a pulsed subnanosecond UV-A laser. Med. Laser Appl. 20, 217-222 (2005).
25. Mandolesi, G., Madeddu, F., Bozzi, Y., Maffei, L. & Ratto, G.M. Acute physiological response of mammalian central neurons to axotomy: ionic regulation and electrical activity. J. Fed. Am. Soc. Exp. Biol. 18, 1934-1936 (2004).
26. O'Brien, G.S. et al. Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos. J. Vis. Exp. pii: 1129; doi:10.3791/1129 (2009).
27. Frostig, R.D. In Vivo Optical Imaging of Brain Function (CRC Press Ltd., Boca Raton, Florida, USA, 2002).
28. Wood, W.B. The Nematode Caenorhabditis Elegans (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA, 1988).
29. Bourgeois, F. & Ben-Yakar, A. Femtosecond laser nanoaxotomy properties and their effect on nerve regeneration in C. elegans. Optics Express 15, 8521-8531 (2007).