Development of a multiplex PCR assay for the simultaneous detection of Clavibacter michiganensis subsp. michiganensis, Pseudomonas syringae pv. tomato and Xanthomonas axonopodis pv. vesicatoria using pure cultures

Journal of Plant Pathology

Volumn 91, Issue 2, 2009, Pages 495-497

  Ozdemir Z ^a  

^a ADNAN MENDERES UNIVERSITY   (Turkey)

Author keywords

Clavibacter michiganensis subsp. michiganensis; Detection; Multiplex PCR; Pseudomonas syringae pv. tomato; Tomato; Xanthomonas axonopodis pv. vesicatoria

Indexed keywords

BACTERIA (MICROORGANISMS); CLAVIBACTER MICHIGANENSIS; CLAVIBACTER MICHIGANENSIS SUBSP. MICHIGANENSIS; LYCOPERSICON ESCULENTUM; PSEUDOMONAS SYRINGAE; PSEUDOMONAS SYRINGAE PV. TOMATO; XANTHOMONAS AXONOPODIS; XANTHOMONAS AXONOPODIS PV. VESICATORIA;

EID: 77749313132     PISSN: 11254653     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. Bereswill S., Bugert P., Volksch B., Ullrich M., Bender C.L., Geider K., 1994.Identification and relatedness of coronatine-producing Pseudomonas syringae pathovars by PCR analysis and sequence determination of the amplification products. Applied and Environmental Microbiology 60: 2924-2930.
2. Bertolini E., Olmos A., López M.M., Cambra M., 2003. Multiplex nested reverse transcription-polymerase chain reaction in a single tube for sensitive and simultaneous detection of four RNA viruses and Pseudomonas savastanoi pv. savastanoi in olive trees. Phytopathology 93: 286-292.
3. Chamberlain J.S., Chamberlain J.R., 1994. Optimization of multiplex PCRs. In: Mullis K.B., Ferré F., Gibbs R.A. (eds). The Polymerase Chain Reaction, pp. 38-46. Birkhäuser, Boston, MS, USA.
4. Dreier J., Bermpohl A., Eichenlaub R., 1995. Southern hybridization and PCR for specific detection of phytopathogenic Clavibacter michiganensis subsp. michiganensis. Phytopathology 85: 462-468.
5. Elnifro E.M., Ashshi A.M., Cooper R.J., Klapper P.E., 2000. Multiplex PCR: optimization and application in diagnostic virology. Clinical Microbiology Reviews 13: 550-570.
6. Fessehaie A., Walcott R., 2005. Simultaneous detection of Acidovorax avenae subsp. citrulli and Didymella bryoniae using multiplex real-time PCR. Phytopathology 95, S29.
7. Glick D.L., Coffey C.M., Sulzinski M.A., (2002) Simultaneous PCR detection of the two major bacterial pathogens of geranium. Journal of Phytopathology 150: 54-59.
8. Hiroyuki U., Tsuda S., 2005. A one-step reverse transcriptionpolymerase chain reaction system for the simultaneous detection and identification of multiple tospovirus infections. Phytopathology 95: 166-171.
9. Johnson K., Walcott R., 2005. A real-time PCR assay for the simultaneous detection of Pepino mosaic virus and Clavibacter michiganensis subsp. michiganensis. Phytopathology 95: S50.
10. Jones J.B., Lacy G.H., Bouzar H., Stall R.E., Schaad N.W., 2004. Reclassification of the xanthomonads associated with bacterial spot disease of tomato and pepper. Systematic and Applied Microbiology. 27: 755-762.
11. King E.O., Ward M.K., Raney D.E., 1954. Two simple media for the demonstration of pyocyanin and fluorescein. Journal of Laboratory and Clinical Medicine 44: 301-307.
12. Leite R.P.Jr., Minsavage G.V., Bonas U., Stall R.E., 1994. Detection and identification of phytopathogenic Xanthomonas strains by amplification of DNA sequences related to the hrp genes of Xanthomonas campestris pv. vesicatoria. Applied and Environmental Microbiology 60: 1068-1077.
13. Menzel W., Jelkman W., Maiss E., 2002. Detection of four apple viruses by multiplex RT-PCR assays with coamplification of plant mRNA as internal control. Journal of Virological Methods 99: 81-92.
14. Özdemir Z., 2005a. Development of a multiplex PCR assay for concurrent detection of Clavibacter michiganensis ssp. michiganensis and Xanthomonas axonopodis pv. vesicatoria. Plant Pathology Journal 4: 133-137.
15. Özdemir Z., 2005b. A multiplex PCR assay for detection of Clavibacter michiganensis subsp. michiganensis, Pseudomonas syringae pv. tomato and Xanthomonas axonopodis pv. vesicatoria. American Phytopathological Society, Northeastern Division Meeting, Geneva 2005: 44.
16. Schaad N.W., Cheong S.S., Tamaki S., Hatziloukas E., Panapoulos N.J., 1995. A combined biological and enzymatic amplification (BIO-PCR) technique to detect Pseudomonas syringae pv. phaseolicola in bean seed extracts. Phytopathology 85: 243-248.
17. Vidaver A.K., 1967. Synthetic and complex media for rapid detection of fluorescence of phytopathogenic pseudomonads: effect of the carbon source. Applied Microbiology 15: 1523-1524.
18. Wilson E.E., Zeitoun F.M., Frederickson D.L., 1967. Bacterial phloem canker, a new disease of Persian walnut trees. Phytopathology 57: 618-621.