Monoclonal antibodies against recombinant hemolysin BL complex of bacillus cereus

Hybridoma

Volumn 29, Issue 1, 2010, Pages 67-71

  Kumar, Thakasi D K ^a   Urs, Radhika Madan ^a   Balakrishna, Konduru ^a   Murali, Harishchandra S ^a   Batra, Harsh V ^a   Bawa, Amarinder S ^a  

^a DEFENCE FOOD RESEARCH LABORATORY   (India)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; BACTERIAL TOXIN; CHIMERIC PROTEIN; CROSS REACTING ANTIBODY; EPITOPE; FREUND ADJUVANT; HEMOLYSIN; MONOCLONAL ANTIBODY; RECOMBINANT PROTEIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBODY PRODUCTION; ARTICLE; BACILLUS CEREUS; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; CONTROLLED STUDY; CROSS REACTION; GENE EXPRESSION PROFILING; GENETIC CODE; IMMUNIZATION; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; WESTERN BLOTTING;

ANIMALS; ANTIBODIES, MONOCLONAL; BACILLUS CEREUS; BACTERIAL PROTEINS; BACTERIAL TOXINS; BLOTTING, WESTERN; ENZYME-LINKED IMMUNOSORBENT ASSAY; FLUORESCENT ANTIBODY TECHNIQUE; HEMOLYSIN PROTEINS; HYBRIDOMAS; IMMUNIZATION; IMMUNOENZYME TECHNIQUES; MICE; MICE, INBRED BALB C; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS;

BACILLUS CEREUS; MURINAE;

EID: 77749267502     PISSN: 15540014     EISSN: None     Source Type: Journal    
DOI: 10.1089/hyb.2009.0060     Document Type: Article

References (17)

1. Beecher DJ, and Macmillan JD: Characterization of the components of haemolysin BL from Bacillus cereus. Infect Immun 1991;59:1778-1784.
2. Beecher DJ, and Wong ACL: Cooperative, synergistic and antagonistic haemolytic interactions between haemolysin BL, phosphatidylcholine phospholipase C and sphingomyelinase from Bacillus cereus. Microbiol 2000;146: 3033-3039.
3. Pruss BMR, Dietrich B, Nibler E, Martlbauer, and Scherer S: The hemolytic enterotoxin HBL is broadly distributed among species of the Bacillus cereus group. Appl Environ Microbiol 1999;65:5436-5442.
4. Beecher DJ, and Macmillan JD: A novel bio component he-molysin from Bacillus cereus. Infect Immun 1990;58:2220-2227.
5. Rusul G, and Yaacob NH: Prevalence of Bacillus cereus in selected foods and detection of enterotoxin using TECRA-VIA and BECT-RPLA. Int J Food Microbiol 1995;25:131-139.
6. Callegan MC, Cochran DC, Kane ST, Gilmore MS, Gominet M, and Lereclus D: Contribution of Membrane-Damaging Toxins to Bacillus Endophthalmitis Pathogenesis. Infect. Immun 2002;70:5381-5389.
7. Beecher DJ, Schoeni JL, and Wong ACL: Enterotoxic activity of hemolysin BL from Bacillus cereus. Infect Immun 1995; 63:632-639.
8. Buchanan RL, and Schultz FJ: Comparison of the Tecra. VIA kit, Oxoid BCET-RPLA kit and CHO cell culture for the detection of Bacillus cereus diarrheal enterotoxin. Lett Appl Microbiol 1994;19:353-356.
9. Day TL, Tatani SR, Notermans S, and Bennett RW: A comparison of ELISA and RPLA for detection of Bacillus cereus diarrhoeal enterotoxin. J Appl Bacteriol 1994;77:9-13.
10. Ryan PA, Macmillan JD, and Zilinskas BA: Molecular cloning and characterization of the genes encoding the L1 and L2 components of hemolysin BL from Bacillus cereus. J Bacteriol 1997;179:2551-2556.
11. Dietrich R, Fella C, Strich S, and Martlbauer E: Production and characterization of monoclonal antibodies against the hemolysin bl enterotoxin complex produced by Bacillus cereus. Appl Environ Microbiol 1999;65(10):4470-4474.
12. Kumar TDK, Murali HS, and Batra HV: Construction of a non toxic chimeric protein (L1-L2-B) of Haemolysin BL from Bacillus cereus and its application in HBL toxin detection. J Microbiol Methods 2008;75:472-477.
13. Engvall E, and Perlman P: Enzyme-linked immunosorbent assay (ELISA): quantitative assay of immunoglobulin G. Immunochemistry 1971;8:871-879.
14. Kohler G, and Milstein C: Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 1975; 256:495-497, PMID 1172191;
15. reproduced in J Immunol 2005; 174:2453-2455.
16. Sambrook J, Fritsch E, and Maniatis A (Eds): Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989.
17. Beecher DJ, and Wong ACL: Identification and analysis of the antigens detected by two commercial Bacillus cereus diarrheal enterotoxin immunoassay kits. Appl Environ Microbiol 1994;60:4614-4616.