Mild hyperthermia prior to electroporation increases transfection efficiency in HCT 116, HeLa S3 and SGC 7901 cells

Biotechnology Letters

Volumn 32, Issue 3, 2010, Pages 367-371

  Wei, Zheng Li ^a   Ogawa, Ryohei ^a   Takasaki, Ichiro ^a   Zhao, Qing Li ^a   Zheng, Hua Chuan ^b   Ahmed, Kanwal ^a   Hassan, Mariame A ^a   Kondo, Takashi ^a  

^a UNIVERSITY OF TOYAMA   (Japan)

^b Liaoning Institute of Basic Medicine   (China)

Author keywords

Efficiency; Electroporation; Mild hyperthermia; Preheating; Transfection

Indexed keywords

COMBINED TREATMENT; ELECTROPORATION; FLUORESCEIN ISOTHIOCYANATE DEXTRANS; LUCIFERASE ACTIVITIES; MILD HYPERTHERMIA; MRNA TRANSCRIPTION; PARTICLE ACCUMULATION; TRANSFECTION EFFICIENCY;

DIELECTRIC DEVICES; GLUCOSE; NUCLEIC ACIDS; SPONTANEOUS EMISSION;

PREHEATING;

DEXTRAN; DRUG DERIVATIVE; FLUORESCEIN ISOTHIOCYANATE; FLUORESCEIN ISOTHIOCYANATE DEXTRAN; LUCIFERASE; MESSENGER RNA;

ARTICLE; CELL STRAIN HCT116; ELECTROPORATION; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; GENETICS; HEAT SHOCK RESPONSE; HELA CELL; HUMAN; INTRACELLULAR SPACE; METABOLISM; METHODOLOGY;

DEXTRANS; ELECTROPORATION; FLUORESCEIN-5-ISOTHIOCYANATE; HCT116 CELLS; HEAT-SHOCK RESPONSE; HELA CELLS; HUMANS; INTRACELLULAR SPACE; LUCIFERASES; RNA, MESSENGER; TRANSCRIPTION, GENETIC; TRANSFECTION;

EID: 77649343001     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-009-0164-5     Document Type: Article

References (14)

1. André F, Mir LM (2004) DNA electrotransfer, its principles and an updated review of its therapeutic applications. Gene Ther 11: S33-S42.
2. Grünhagen DJ, de Wilt JH, van Geel AN et al (2006) Isolated limb perfusion for melanoma patients-a review of its indications and the role of tumour necrosis factor-alpha. Eur J Surg Oncol 32: 371-380.
3. Hildebrandt B, Wust P, Ahlers O et al (2002) The cellular and molecular basis of hyperthermia. Crit Rev Oncol Hematol 43: 33-56.
4. Lechardeur D, Lukacs GL (2002) Intracellular barriers to non-viral gene transfer. Curr Gene Ther 2: 183-194.
5. Li GC, He F, Ling CC (2006) Hyperthermia and gene therapy, potential use of microPET imaging. Int J Hyperthermia 22: 215-221.
6. Mir LM, Bureau MF, Rangara R et al (1998) Long-term, high level in vivo gene expression after electric pulse-mediated gene transfer into skeletal muscle. C R Acad Sci III 321: 893-899.
7. Molnar MJ, Gilbert R, Lu Y et al (2004) Factors influencing the efficacy, longevity, and safety of electroporation-assisted plasmid-based gene transfer into mouse muscles. Mol Ther 10: 447-455.
8. Murthy SN, Sen A, Zhao YL et al (2004) Temperature influences the postelectroporation permeability state of the skin. J Pharm Sci 93: 908-915.
9. Mushiake H, Aoe M, Washio K et al (2002) Enhancement of gene transduction efficiency in cancer cells using cationic liposome with hyperthermia. Acta Med Okayama 56: 35-42.
10. Ogawa R, Kagiya G, Feril LB Jr et al (2004) Ultrasound mediated intravesical transfection enhanced by treatment with lidocaine or heat. J Urol 172: 1469-1473.
11. Omlor G, Gross G, Ecker KW et al (1992) Optimization of isolated hyperthermic limb perfusion. World J Surg 16: 1117-1119.
12. Rols MP (2006) Electropermeabilization, a physical method for the delivery of therapeutic molecules into cells. Biochim Biophys Acta 1758: 423-428.
13. Schertzer JD, Plant DR, Lynch GS (2006) Optimizing plasmid-based gene transfer for investigating skeletal muscle structure and function. Mol Ther 13: 795-803.
14. Takasaki I, Takarada S, Fukuchi M et al (2007) Identification of genetic networks involved in the cell growth arrest and differentiation of a rat astrocyte cell line RCG-12. J Cell Biochem 102: 1472-1485.