Optimization of a dendritic cell-based assay for the in vitro priming of naïve human CD4+ T cells

Journal of Immunological Methods

Volumn 353, Issue 1-2, 2010, Pages 8-19

  Moser, Janice M ^a   Sassano, Emily R ^a   Leistritz, Del C ^a   Eatrides, Jennifer M ^a   Phogat, Sanjay ^b   Koff, Wayne ^b   Drake, Donald R ^a  

^a VaxDesign Corporation   (United States)

^b INTERNATIONAL AIDS VACCINE INITIATIVE   (United States)

Author keywords

Antigen presentation; Dendritic cells; Human; In vitro assays; T cells

Indexed keywords

GAMMA INTERFERON; INTERLEUKIN 17; INTERLEUKIN 2; INTERLEUKIN 21; INTERLEUKIN 5; TUMOR NECROSIS FACTOR ALPHA; YELLOW FEVER VACCINE;

ARTICLE; CD4+ T LYMPHOCYTE; CELL ASSAY; CELL MATURATION; CELL STIMULATION; CELLULAR IMMUNITY; CHEMOSENSITIZATION; COCULTURE; CONTROLLED STUDY; CULTURE OPTIMIZATION; CYTOKINE PRODUCTION; DENDRITIC CELL; HELPER CELL; HUMAN; HUMAN CELL; IN VITRO STUDY; MEMORY CELL; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; PURIFICATION; REGULATORY T LYMPHOCYTE;

ANTIGEN PRESENTATION; CD4-POSITIVE T-LYMPHOCYTES; CELL SEPARATION; CELLS, CULTURED; COCULTURE TECHNIQUES; DENDRITIC CELLS; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; IMMUNOLOGIC MEMORY; INTERFERON-GAMMA; INTERLEUKINS; LYMPHOCYTE ACTIVATION; T-LYMPHOCYTES, HELPER-INDUCER; T-LYMPHOCYTES, REGULATORY; TUMOR NECROSIS FACTOR-ALPHA; VACCINES, SUBUNIT; YELLOW FEVER VACCINE;

EID: 77649275176     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2009.11.006     Document Type: Article

References (43)

1. Ahmed R., Gray D. Immunological memory and protective immunity: understanding their relation. Science 1996, 272:54.
2. Aiba S., Tagami H. Dendritic cell activation induced by various stimuli, e.g. exposure to microorganisms, their products, cytokines, and simple chemicals as well as adhesion to extracellular matrix. J. Dermatol. Sci. 1998, 20:1.
3. Banchereau J., Briere F., Caux C., Davoust J., Lebecque S., Liu Y.J., Pulendran B., Palucka K. Immunobiology of dendritic cells. Annu. Rev. Immunol. 2000, 18:767.
4. Bonasio R., von Andrian U.H. Generation, migration and function of circulating dendritic cells. Curr. Opin. Immunol. 2006, 18:503.
5. Boscardin S.B., Hafalla J.C., Masilamani R.F., Kamphorst A.O., Zebroski H.A., Rai U., Morrot A., Zavala F., Steinman R.M., Nussenzweig R.S., Nussenzweig M.C. Antigen targeting to dendritic cells elicits long-lived T cell help for antibody responses. J. Exp. Med. 2006, 203:599.
6. Corinti S., Medaglini D., Prezzi C., Cavani A., Pozzi G., Girolomoni G. Human dendritic cells are superior to B cells at presenting a major histocompatibility complex class II-restricted heterologous antigen expressed on recombinant Streptococcus gordonii. Infect. Immun. 2000, 68:1879.
7. de Jong J.M., Schuurhuis D.H., Ioan-Facsinay A., Welling M.M., Camps M.G., van der Voort E.I., Huizinga T.W., Ossendorp F., Verbeek J.S., Toes R.E. Dendritic cells, but not macrophages or B cells, activate major histocompatibility complex class II-restricted CD4+ T cells upon immune-complex uptake in vivo. Immunology 2006, 119:499.
8. Drescher K.M., Sosnowska D. Being a mouse in a man's world: what TMEV has taught us about human disease. Front. Biosci. 2008, 13:3775.
9. Duda P.W., Krieger J.I., Schmied M.C., Balentine C., Hafler D.A. Human and murine CD4 T cell reactivity to a complex antigen: recognition of the synthetic random polypeptide glatiramer acetate. J. Immunol. 2000, 165:7300.
10. Forster R., Schubel A., Breitfeld D., Kremmer E., Renner-Muller I., Wolf E., Lipp M. CCR7 coordinates the primary immune response by establishing functional microenvironments in secondary lymphoid organs. Cell 1999, 99:23.
11. Frentsch M., Arbach O., Kirchhoff D., Moewes B., Worm M., Rothe M., Scheffold A., Thiel A. Direct access to CD4+ T cells specific for defined antigens according to CD154 expression. Nat. Med. 2005, 11:1118.
12. Garside P., Ingulli E., Merica R.R., Johnson J.G., Noelle R.J., Jenkins M.K. Visualization of specific B and T lymphocyte interactions in the lymph node. Science 1998, 281:96.
13. Gaucher D., Therrien R., Kettaf N., Angermann B.R., Boucher G., Filali-Mouhim A., Moser J.M., Mehta R.S., Drake D.R., Castro E., Akondy R., Rinfret A., Yassine-Diab B., Said E.A., Chouikh Y., Cameron M.J., Clum R., Kelvin D., Somogyi R., Greller L.D., Balderas R.S., Wilkinson P., Pantaleo G., Tartaglia J., Haddad E.K., Sekaly R.P. Yellow fever vaccine induces integrated multilineage and polyfunctional immune responses. J. Exp. Med. 2008, 205:3119.
14. Gupta U.D., Katoch V.M. Animal models of tuberculosis. Tuberc. (Edinb.) 2005, 85:277.
15. Jonuleit H., Schmitt E., Kakirman H., Stassen M., Knop J., Enk A.H. Infectious tolerance: human CD25(+) regulatory T cells convey suppressor activity to conventional CD4(+) T helper cells. J. Exp. Med. 2002, 196:255.
16. Kirchner H., Tosato G., Blaese R.M., Broder S., Magrath I.T. Polyclonal immunoglobulin secretion by human B lymphocytes exposed to Epstein-Barr virus in vitro. J. Immunol. 1979, 122:1310.
17. Kwok W.W., Yang J., James E., Bui J., Huston L., Wiesen A.R., Roti M. The anthrax vaccine adsorbed vaccine generates protective antigen (PA)-Specific CD4+ T cells with a phenotype distinct from that of naive PA T cells. Infect. Immun. 2008, 76:4538.
18. Langenkamp A., Casorati G., Garavaglia C., Dellabona P., Lanzavecchia A., Sallusto F. T cell priming by dendritic cells: thresholds for proliferation, differentiation and death and intraclonal functional diversification. Eur. J. Immunol. 2002, 32:2046.
19. Leitenberg D., Bottomly K. Regulation of naive T cell differentiation by varying the potency of TCR signal transduction. Semin. Immunol. 1999, 11:283.
20. Li Pira G., Oppezzi L., Seri M., Westby M., Caroli F., Fenoglio D., Lancia F., Ferraris A., Bottone L., Valle M.T., Kunkl A., Romeo G., Dalgleish A.G., Manca F. Repertoire breadth of human CD4+ T cells specific for HIV gp120 and p66 (primary antigens) or for PPD and tetanus toxoid (secondary antigens). Hum. Immunol. 1998, 59:137.
21. Macatonia S.E., Patterson S., Knight S.C. Primary proliferative and cytotoxic T-cell responses to HIV induced in vitro by human dendritic cells. Immunology 1991, 74:399.
22. Mehta-Damani A., Markowicz S., Engleman E.G. Generation of antigen-specific CD8+ CTLs from naive precursors. J. Immunol. 1994, 153:996.
23. Moon J.J., Chu H.H., Pepper M., McSorley S.J., Jameson S.C., Kedl R.M., Jenkins M.K. Naive CD4(+) T cell frequency varies for different epitopes and predicts repertoire diversity and response magnitude. Immunity 2007, 27:203.
24. Muller G., Hopken U.E., Lipp M. The impact of CCR7 and CXCR5 on lymphoid organ development and systemic immunity. Immunol. Rev. 2003, 195:117.
25. Ohl L., Henning G., Krautwald S., Lipp M., Hardtke S., Bernhardt G., Pabst O., Forster R. Cooperating mechanisms of CXCR5 and CCR7 in development and organization of secondary lymphoid organs. J. Exp. Med. 2003, 197:1199.
26. Osugi Y., Vuckovic S., Hart D.N. Myeloid blood CD11c(+) dendritic cells and monocyte-derived dendritic cells differ in their ability to stimulate T lymphocytes. Blood 2002, 100:2858.
27. Querec T., Bennouna S., Alkan S., Laouar Y., Gorden K., Flavell R., Akira S., Ahmed R., Pulendran B. Yellow fever vaccine YF-17D activates multiple dendritic cell subsets via TLR2, 7, 8, and 9 to stimulate polyvalent immunity. J. Exp. Med. 2006, 203:413.
28. Raulf-Heimsoth M. T cell - primary culture from peripheral blood. Methods Mol. Med. 2008, 138:17.
29. Reinhardt B., Jaspert R., Niedrig M., Kostner C., L'Age-Stehr J. Development of viremia and humoral and cellular parameters of immune activation after vaccination with yellow fever virus strain 17D: a model of human flavivirus infection. J. Med. Virol. 1998, 56:159.
30. Reis E.A., Mauadi Carmo T.A., Athanazio R., Reis M.G., Harn D.A. Schistosoma mansoni triose phosphate isomerase peptide MAP4 is able to trigger naive donor immune response towards a type-1 cytokine profile. Scand. J. Immunol. 2008, 68:169.
31. Rizzuto G.A., Merghoub T., Hirschhorn-Cymerman D., Liu C., Lesokhin A.M., Sahawneh D., Zhong H., Panageas K.S., Perales M.A., Altan-Bonnet G., Wolchok J.D., Houghton A.N. Self-antigen-specific CD8+ T cell precursor frequency determines the quality of the antitumor immune response. J. Exp. Med. 2009, 206:849.
32. Rossi M., Young J.W. Human dendritic cells: potent antigen-presenting cells at the crossroads of innate and adaptive immunity. J. Immunol. 2005, 175:1373.
33. Sallusto F., Lanzavecchia A. Heterogeneity of CD4+ memory T cells: functional modules for tailored immunity. Eur. J. Immunol. 2009, 39:2076.
34. Sallusto F., Geginat J., Lanzavecchia A. Central memory and effector memory T cell subsets: function, generation, and maintenance. Annu. Rev. Immunol. 2004, 22:745.
35. Schakel K., Mayer E., Federle C., Schmitz M., Riethmuller G., Rieber E.P. A novel dendritic cell population in human blood: one-step immunomagnetic isolation by a specific mAb (M-DC8) and in vitro priming of cytotoxic T lymphocytes. Eur. J. Immunol. 1998, 28:4084.
36. Schanen B.C., Drake D.R. A novel approach for the generation of human dendritic cells from blood monocytes in the absence of exogenous factors. J. Immunol. Methods 2008, 335:53.
37. Stagg A.J., Elsley W.A., Pickett M.A., Ward M.E., Knight S.C. Primary human T-cell responses to the major outer membrane protein of Chlamydia trachomatis. Immunology 1993, 79:1.
38. Thornton A.M., Shevach E.M. CD4+CD25+ immunoregulatory T cells suppress polyclonal T cell activation in vitro by inhibiting interleukin 2 production. J. Exp. Med. 1998, 188:287.
39. Thornton A.M., Shevach E.M. Suppressor effector function of CD4+CD25+ immunoregulatory T cells is antigen nonspecific. J. Immunol. 2000, 164:183.
40. Victorino R.M., Hodgson H.J. Relationship between T cell subpopulations and the mitogen responsiveness and suppressor cell function of peripheral blood mononuclear cells in normal individuals. Clin. Exp. Immunol. 1980, 42:571.
41. Watkins D.I., Burton D.R., Kallas E.G., Moore J.P., Koff W.C. Nonhuman primate models and the failure of the Merck HIV-1 vaccine in humans. Nat. Med. 2008, 14:617.
42. Young J.W., Inaba K. Dendritic cells as adjuvants for class I major histocompatibility complex-restricted antitumor immunity. J. Exp. Med. 1996, 183:7.
43. Zhao D.M., Thornton A.M., DiPaolo R.J., Shevach E.M. Activated CD4+CD25+ T cells selectively kill B lymphocytes. Blood 2006, 107:3925.