Combining positional scanning peptide libraries, HLA-DR transfectants and bioinformatics to dissect the epitope spectrum of HLA class II cross-restricted CD4+ T cell clones

Journal of Immunological Methods

Volumn 353, Issue 1-2, 2010, Pages 93-101

  Sospedra, Mireia ^a,b   Zhao, Yingdong ^c   Giulianotti, Marc ^d   Simon, Richard ^c   Pinilla, Clemencia ^d,e   Martin, Roland ^a,b  

^a NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE   (United States)

^b UNIVERSITY MEDICAL CENTER HAMBURG EPPENDORF   (Germany)

^c NATIONAL CANCER INSTITUTE   (United States)

^d Mixture Sciences Inc   (United States)

^e TORREY PINES INSTITUTE FOR MOLECULAR STUDIES   (United States)

Author keywords

Antigen presentation; Combinatorial peptide libraries; MHC; Specificity; T cells

Indexed keywords

EPITOPE; HLA ANTIGEN CLASS 1; HLA ANTIGEN CLASS 2; HLA DR ANTIGEN;

ACCURACY; ANTIBODY LIBRARY; ANTIGEN PRESENTING CELL; ANTIGEN RECOGNITION; ARTICLE; B LYMPHOCYTE; BIOINFORMATICS; BIOMETRY; CD4+ T LYMPHOCYTE; CELL CLONE; CELL TYPE; CONTROLLED STUDY; CROSS REACTION; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; PERIPHERAL BLOOD MONONUCLEAR CELL; PREDICTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUALITATIVE ANALYSIS; REPRODUCIBILITY; T LYMPHOCYTE;

ANTIGEN PRESENTATION; ANTIGEN-PRESENTING CELLS; B-LYMPHOCYTES; CD4-POSITIVE T-LYMPHOCYTES; CELL PROLIFERATION; CELLS, CULTURED; COMPUTATIONAL BIOLOGY; CYTOKINES; EPITOPE MAPPING; EPITOPES; HLA-DR ANTIGENS; HUMANS; LYMPHOCYTE ACTIVATION; PEPTIDE LIBRARY; T-CELL ANTIGEN RECEPTOR SPECIFICITY; TRANSFECTION;

EID: 77649272880     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2009.12.006     Document Type: Article

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