A new diagnostic system for ultra-sensitive and specific detection and quantification of Candidatus Liberibacter asiaticus, the bacterium associated with citrus Huanglongbing

Journal of Microbiological Methods

Volumn 81, Issue 1, 2010, Pages 17-25

  Lin, Hong ^a   Chen, Chuanwu ^b   Doddapaneni, Harshavardhan ^c   Duan, Yongping ^d   Civerolo, Edwin L ^a   Bai, Xianjin ^e   Zhao, Xiaolong ^b  

^a U S DEPARTMENT OF AGRICULTURE   (United States)

^b Guangxi Citrus Research Institute   (China)

^c UNIVERSITY OF IOWA   (United States)

^d AGRICULTURAL RESEARCH SERVICE   (United States)

^e NATIONAL ENGINEERING RESEARCH CENTER FOR NON FOOD BIOREFINERY   (China)

Author keywords

Candidatus Liberibacter asiaticus; Citrus; Huanglongbing; TaqMan PCR

Indexed keywords

ANALYZER; ARTICLE; BACTERIUM IDENTIFICATION; CANDIDATUS LIBERIBACTER ASIATICUS; CITRUS FRUIT; CONTAMINATION; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; FLUORESCENCE; GRAM NEGATIVE BACTERIUM; HIGH THROUGHPUT SCREENING; HUANGLONGBING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT PATHOGEN INTERACTION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RELIABILITY; SENSITIVITY AND SPECIFICITY; SIGNAL DETECTION; TEMPERATURE SENSITIVITY;

CITRUS; DNA PRIMERS; OLIGONUCLEOTIDE PROBES; POLYMERASE CHAIN REACTION; RHIZOBIACEAE; SENSITIVITY AND SPECIFICITY;

BACTERIA (MICROORGANISMS); CANDIDATUS LIBERIBACTER ASIATICUS; CITRUS; HEXAPODA;

EID: 77649235902     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2010.01.014     Document Type: Article

References (44)

1. Ali A., Auschill T.M., Braun G., Hellwig E., and Arweiler N.B. Overestimation of Streptococcus mutans prevalence by nested PCR detection of the 16 S rRNA gene. J. Med. Microbiol. 55 (2006) 109-113
2. Anderson T.P., Beynon K.A., and Murdoch D.R. Comparison of real-time PCR and conventional hemi-nested PCR for the detection of Bordetella pertussis in nasopharyngeal samples. Clin. Microbiol. Infect. 9 (2003) 746-749
3. Bastianel C., Garnier-Semancik M., Renaudin J., Bové J.M., and Eveillard S. Diversity of "Candidatus Liberibacter asiaticus," based on the omp gene sequence. Appl. Environ. Microbiol. 71 (2005) 6473-6478
4. Beattie G.A.C., Holford P., Mabberley D.J., Haigh A.M., and Broadbent P. On the Origins of Citrus, Huanglongbing, Diaphorina citri and Trioza erytreae International Conference of Huanglongbing. Orlando, USA 25-57 (2008)
5. Benyon L., Zhou L., Weathersbee A., and Duan Y. Nested PCR is essential for the detection of extremely low titer of 'Candidatus Liberibacter asiaticus' from citrus and its vector psyllid Diaphorina citri. Phytopathology 98 (2008) S21
6. Bové J.M. Huanglongbing A destructive, newly-emerging, century-old disease of citrus. J. Plant Pathol. 88 (2006) 7-37
7. Bové J.M., Calavan E.C., Capoor S.P., Cortez R.E., and Schwarz R.E. Influence of temperature on symptoms of California stubborn, South African greening, Indian citrus decline and Philippines leaf mottling diseases. Proc. 6th Conf. IOCV, Univ. Calif., Div. Agr. Sci., Riverside. CA (1974) 12-15
8. Catling H.D. The bionomics of the South African citrus Psylla Trioza-erytreae (Del Guercio) (Homoptera Psyllidae) Part 1. The influence of the flushing rhythm of citrus-D and factors which regulate flushing. J. Ent. Soc. S. Afr. 32 (1969) 191-208
9. Coletta-Filho H.D., Targon M.L.P.N., Takita M.A., De Negri Jr. J.D., Pompeu J., M.A., and M. First report of the causal agent of Huanglongbing "Candidatus Liberibacter asiaticus" in Brazil. Plant Dis. 88 (2004) 1382
10. Deng X., Zhou G., Li H., Chen J., and Civerolo L.E. Nested-PCR detection and sequence confirmation of 'Candidatus Liberibacter asiaticus' from Murraya paniculata in Guangdong, China. Dis. Notes 91 (2007) 1051
11. Ding F., Yi G., and Wang G. Research on the PCR and Nested 2 PCR Detection of Citrus Huanglongbing. Acta Horticult. Sin. 31 (2004) 803-806
12. Ding F., Wang G., Yi G., Zhong Y., Zeng J., and Zhou B. Infection of Wampee and lemon by the citrus huanglongbing pathogen 'Candidatrus Liberibacter asiaticus' in China. J. Plant Pathol. 87 (2005) 207-212
13. Doddapaneni H., Liao H., Lin H., Bai X., Zhao X., Civerolo E.L., Irey M., Coletta-Filho H., and Pietersen G. Comparative Phylogenomics and Multi-gene Cluster Analyses of the Citrus Huanglongbing (HLB)-associated Bacterium 'Candidatus Liberibacter' (2008) BMC research notes. 1,72
14. Duan Y., Zhou L., Hall D.G., Li W., Doddapaneni H., Lin H., Liu L., Vahling C.M., Gabriel D.W., Williams K.P., Dickerman A., Sun Y., and Gottwald T. Complete genome sequence of citrus huanglongbing bacterium, 'Candidatus Liberibacter asiaticus' obtained through metagenomics. Mol. Plant Microbe Interact. 22 (2009) 1011-1020
15. Farrell D.J. Evaluation of Amplocor Neisseria gonorrhoeae PCR using cppB nested PCR and 16S rRNA PCR. J. Clin. Microbiol. (1999) 386-390
16. Forsman A., Uzameckis D., Ronnblom L., Baecklund E., Aleskog A., Bindra A., Pipkorn R., Lejniece S., Kozireva S., Murovska M., and Blomberg J. Single-tube nested quantitative PCR a rational and sensitive technique for detection of retroviral DNA. Application to RERV-H/HRV-5 and confirmation of its rabbit origin. J. Virol. Methods 111 (2003) 1-11
17. Gottwald T.R., Dixon W., Parnell S.R., T., and R. Huanglongbing: the dragon arrives in the USA. Huanglongbing-greening International Workshop Proceedings (2006) 13-14
18. Gottwald T.R., Da Graca J.V., and Bassanezi R.B. Citrus huanglongbing the pathogen and its epidemiology, and impact. Plant Healthy Progress (2007)
19. Halbert S.E. The discovery of huanglongbing in Florida. Proceedings of 2nd International Citrus Canker and Huanglongbing Research Workshop Florida Citrus Mutual, Orlando H-3 (2005)
20. Halbert, S. E., and Manjunath, K. L. 2004. Asian citrus psyllid (Sternorrhyncha: Psyllidae) and greening disease of citrus, a literature review and assessment of risk in Florida. Florida Entomologist 87, 330-353.
21. Hocquellet A., Toorawa P., Bové J.M., and Garnier M. Detection and identification of the two "Candidatus Liberobacter sp" associated with citrus huanglongbing by PCR amplification of ribosomal protein genes of the beta operon. Mol. Cell Probes 13 (1999) 373-379
22. Hung T.H., Wu M.L., J., and S.H. Development of a rapid method for the diagnosis of citrus greening disease using the polymerase chain reaction. J. Phytopathol. 147 (1999) 599-604
23. Kunimasa K., Truc Nguyen T.N., Lan Bui T.N., Hong Le T.T., and Masatoshi O. Quantification of DNA of citrus huanglongbing pathogen in diseased leaves using competitive PCR. J. Gen. Plant Pathol. 72 (2006) 355-359
24. Li T., and Ke C. Detection of bearing rate of Liberobacter asiaticum in citrus psylla and its host plant Murraya paniculata by nested PCR. Acta Phytopathol. Sin. 29 (2002) 31-35
25. Li W., Hartung J.S., and Levy L. Quantitative real-time PCR for detection and identification of 'Candidatus Liberibacter species' associated with citrus huanglongbing. J. Microbiol. Methods 66 (2006) 104-115
26. Lin K.H. Yellow shoot of citrus in Chinese. Acta Phytopathol. Sin. 2 (1956) 1-12
27. Lin H., Doddapaneni H., Bai X., Yao J., Zhao X., and Civerolo E.L. Acquisition of uncharacterized sequences from 'Candidatus Liberibacter', an unculturable bacterium, using an improved genomic walking method. Mol. Cell Probes. 22 (2008) 30-37
28. Llop P., Bonaterra A., Penalver J., and Lopez M.M. Development of a highly sensitive nested-PCR procedure using a single closed tube for detection of Erwinia amylovora in asymptomatic plant material. Appl. Environ. Microbiol. 66 (2000) 2071-2078
29. Manjunath K.L., Halbert S.E., Ramadugu C., Webb S., and Lee R.F. Detection of 'Candidatus Liberibacter asiaticus' in Diaphorina citri and its importance in the management of Citrus huanglongbing in Florida. Phytopathology 98 (2008) 387-396
30. Martínez, Y., Llauger, R., Batista, L., Luis, M., Iglesia, A., Collazo, C., Peña, I., Casín, J. C., Cueto, J., and Tablada, L. M. 2008. First report of 'Candidatus Liberibacter asiaticus' associated with Huanglongbing in Cuba. BS Plant Pathology 18, August 2008 to January 2009.
31. Matos L., Hilf M.E., and Camejo J. First report of 'Candidatus Liberibacter asiaticus' associated with citrus Huanglongbing in the Dominican Republic. Dis. Notes 93 (2009) 668
32. McClean A.P.D. The efficiency of citrus Psylla Trioza-erytreae as a vector of greening disease of citrus. Phytophylactica 6 (1974) 45-54
33. McClean A.P.D., and Oberholzer P.C.J. Citrus psylla, a vector of the greening disease of sweet orange. S. Afr. J. Agri. Sci. 8 (1965) 297-298
34. NAPPO, 2009. Detection of Huanglongbing 'Candidatus Liberibacter asiaticus' in the municipality of Tizimin, Yucatan, Mexico. Phytosanitary alert system. Official pest reports, http://www.pestalert.org/oprDetail.cfm?oprID=384.
35. Okuda M., Kawano S., Murayama Y., and Iwanami T. Conditions for loop-mediated isothermal amplification LAMP and a nonmacerating DNA extraction method to assay for huanglongbing citrus greening disease. J. Jpn. Phytopathol. 74 (2008) 316-320
36. Rolain J.-M., Fenollar F., and Raoult D. False positive PCR detection of Tropheryma whipplei in the saliva of healthy people. BMC Microbiol. (2007) 10.1186/1471-2180-7-48
37. Sechler A., Schuenzel E.L., Cooke P., Donnua S., Thaveechai N., Postnikova E., Stone A.L., Schneider W.L., Damsteegt V.D., and Schaad N.W. Cultivation of 'Candidatus Liberibacter asiaticus', 'Ca. L. africanus', and 'Ca. L. americanus' associated with huanglongbing. Phytopathology 99 (2009) 480-486
38. Teixeira D.d.C., Danet J.L., Eveillard S., Martins E.C., Junior W.C.d.J., Yamamoto P.T., Lopes S.A., Bassanezi R.B., Ayres A.J., Saillard C., and Bové J.M. Citrus huanglongbing in Sao Paulo State, Brazil PCR detection of the 'Candidatus' Liberibacter species associated with the disease. Mol. Cell Probes 19 (2005) 173-179
39. Teixeira D.C., Saillard C., Couture C., Martins E.C., Wulff N.A., Eveillard-Jagoueix S., Yamamoto P.T., Ayres A.J., and Bové J.M. Distribution and quantification of Candidatus Liberibacter americanus, agent of huanglongbing disease of citrus in Sao Paulo State, Brasil, in leaves of an affected sweet orange tree as determined by PCR. Mol. Cell Probes 22 (2008) 139-150
40. Tjhie J., van Belle A., Dessens-Kroon M., and van Soolingen D. Misidentification and diagnostic delay caused by a false-positive amplified Mycobacterium tuberculosis direct test in an immunocompetent patient with a Mycobacterium celatum infection. J. Clin. Microbiol. 39 (2001) 2311-2312
41. Varma A., Ahlawat Y.S., Chakraborty N.K., Garnier M., and Bové J. Detection of the greening BLO by electron microscopy, DNA hybridization and ELISA in citrus leaves with and without mottle from various regions in India. Proceedings of 12th Conference IOCV, IOCV, Riverside (1993) 280-286
42. Villechanoux S., Garnier M., and Bové J.M. Purification of the bacterium-like organism associated with greening disease of citrus by immunoaffinity chromatography and monoclonal antibodies. Curr. Mocrobiol. 21 (1990) 175-180
43. Villechanoux S., Garnier M., Renaudin J., and Bové J.M. Detection of several strains of the bacterium-like organism of citrus greening disease by DNA probes. Curr. Microbiol. 24 (1992) 89-96
44. Villechanoux S., Garnier M., Laigret F., Renaudin J., and Bové J.M. The genome of the non-cultured, bacterial-like organism associated with citrus greening disease contains the nusG-rplKAJL-rpoBC gene cluster and the gene for a bacteriophage type DNA polymerase. Curr. Microbiol. 26 (1993) 161-166