Direct interaction between Utp8p and Utp9p contributes to rRNA processing in budding yeast

Biochemical and Biophysical Research Communications

Volumn 393, Issue 2, 2010, Pages 297-302

  Huang, Yu Ching ^a   Tseng, Shun Fu ^a   Tsai, Hung Ji ^a   Lenzmeier, Brian A ^b   Teng, Shu Chun ^a  

^a NATIONAL TAIWAN UNIVERSITY   (Taiwan)

^b BUENA VISTA UNIVERSITY   (United States)

Author keywords

Pre rRNA process; SSU complex; tRNA; U3 snoRNA; Utp8; Utp9

Indexed keywords

FUNGAL PROTEIN; RIBOSOME RNA; RNA 18S; SMALL NUCLEOLAR RNA; SMALL SUBUNIT RIBOSOMAL RNA; UNCLASSIFIED DRUG; UTP8P PROTEIN; UTP9P PROTEIN;

ARTICLE; BIOSYNTHESIS; CONTROLLED STUDY; FUNGAL STRAIN; GENE INTERACTION; GENE MUTATION; IN VIVO STUDY; NONHUMAN; PRIORITY JOURNAL; RNA PROCESSING; YEAST; YEAST CELL;

NUCLEAR PROTEINS; RNA PRECURSORS; RNA, RIBOSOMAL; RNA, RIBOSOMAL, 18S; RNA, SMALL NUCLEOLAR; RNA-BINDING PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

SACCHAROMYCES CEREVISIAE; SACCHAROMYCETALES;

EID: 77649190927     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2010.02.003     Document Type: Article

References (24)

1. Kressler D., Linder P., and De La Cruz J. Protein trans-acting factors involved in ribosome biogenesis in Saccharomyces cerevisiae. Mol. Cell. Biol. 19 (1999) 7897-7912
2. Dragon F., Gallagher J.E., Compagnone-Post P.A., Mitchell B.M., Porwancher K.A., Wehner K.A., Wormsley S., Settlage R.E., Shabanowitz J., Osheim Y., Beyer A.L., Hunt D.F., and Baserga S.J. A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis. Nature 417 (2002) 967-970
3. Beltrame M., Henry Y., and Tollervey D. Mutational analysis of an essential binding site for the U3 snoRNA in the 5′ external transcribed spacer of yeast pre-rRNA. Nucleic Acids Res. 22 (1994) 5139-5147
4. Beltrame M., and Tollervey D. Base pairing between U3 and the pre-ribosomal RNA is required for 18S rRNA synthesis. EMBO J. 14 (1995) 4350-4356
5. Gallagher J.E., Dunbar D.A., Granneman S., Mitchell B.M., Osheim Y., Beyer A.L., and Baserga S.J. RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components. Genes Dev. 18 (2004) 2506-2517
6. Krogan N.J., Peng W.T., Cagney G., Robinson M.D., Haw R., Zhong G., Guo X., Zhang X., Canadien V., Richards D.P., Beattie B.K., Lalev A., Zhang W., Davierwala A.P., Mnaimneh S., Starostine A., Tikuisis A.P., Grigull J., Datta N., Bray J.E., Hughes T.R., Emili A., and Greenblatt J.F. High-definition macromolecular composition of yeast RNA-processing complexes. Mol. Cell 13 (2004) 225-239
7. Arts G.J., Fornerod M., and Mattaj I.W. Identification of a nuclear export receptor for tRNA. Curr. Biol. 8 (1998) 305-314
8. Hellmuth K., Lau D.M., Bischoff F.R., Kunzler M., Hurt E., and Simos G. Yeast Los1p has properties of an exportin-like nucleocytoplasmic transport factor for tRNA. Mol. Cell. Biol. 18 (1998) 6374-6386
9. Kutay U., Lipowsky G., Izaurralde E., Bischoff F.R., Schwarzmaier P., Hartmann E., and Gorlich D. Identification of a tRNA-specific nuclear export receptor. Mol. Cell 1 (1998) 359-369
10. Takano A., Endo T., and Yoshihisa T. tRNA actively shuttles between the nucleus and cytosol in yeast. Science 309 (2005) 140-142
11. Sarkar S., and Hopper A.K. TRNA nuclear export in Saccharomyces cerevisiae: in situ hybridization analysis. Mol. Biol. Cell 9 (1998) 3041-3055
12. Eswara M.B., McGuire A.T., Pierce J.B., and Mangroo D. Utp9p facilitates Msn5p-mediated nuclear reexport of retrograded tRNAs in Saccharomyces cerevisiae. Mol. Biol. Cell 20 (2009) 5007-5025
13. Strub B.R., Eswara M.B., Pierce J.B., and Mangroo D. Utp8p is a nucleolar tRNA-binding protein that forms a complex with components of the nuclear tRNA export machinery in Saccharomyces cerevisiae. Mol. Biol. Cell 18 (2007) 3845-3859
14. Steiner-Mosonyi M., Leslie D.M., Dehghani H., Aitchison J.D., and Mangroo D. Utp8p is an essential intranuclear component of the nuclear tRNA export machinery of Saccharomyces cerevisiae. J. Biol. Chem. 278 (2003) 32236-32245
15. James P., Halladay J., and Craig E.A. Genomic libraries and a host strain designed for highly efficient two-hybrid selection in yeast. Genetics 144 (1996) 1425-1436
16. Ausubel F.M., Brent R., Kingston R.E., Moore D.D., Seidman J.G., Smith J.A., and Struhl K. Current Protocols in Molecular Biology (1995), John Wiley & Sons, Inc.
17. Lee S.J., and Baserga S.J. Imp3p and Imp4p, two specific components of the U3 small nucleolar ribonucleoprotein that are essential for pre-18S rRNA processing. Mol. Cell. Biol. 19 (1999) 5441-5452
18. Jones D.T. Protein secondary structure prediction based on position-specific scoring matrices. J. Mol. Biol. 292 (1999) 195-202
19. Spee J.H., De Vos W.M., and Kuipers O.P. Efficient random mutagenesis method with adjustable mutation frequency by use of PCR and dITP. Nucleic Acids Res. 21 (1993) 777-778
20. Muhlrad D., Hunter R., and Parker R. A rapid method for localized mutagenesis of yeast genes. Yeast 8 (1992) 79-82
21. Tollervey D. A yeast small nuclear RNA is required for normal processing of pre-ribosomal RNA. EMBO J. 6 (1987) 4169-4175
22. Hughes J.M., and Ares Jr. M. Depletion of U3 small nucleolar RNA inhibits cleavage in the 5′ external transcribed spacer of yeast pre-ribosomal RNA and impairs formation of 18S ribosomal RNA. EMBO J. 10 (1991) 4231-4239
23. Gallagher J.E., and Baserga S.J. Two-hybrid Mpp10p interaction-defective Imp4 proteins are not interaction defective in vivo but do confer specific pre-rRNA processing defects in Saccharomyces cerevisiae. Nucleic Acids Res. 32 (2004) 1404-1413
24. Guthrie C., Nashimoto H., and Nomura M. Structure and function of E. coli ribosomes. 8. Cold-sensitive mutants defective in ribosome assembly. Proc. Natl. Acad. Sci. USA 63 (1969) 384-391