Optimized microplate β-1,3-glucanase assay system for Trichoderma spp. screening

Journal of Microbiological Methods

Volumn 81, Issue 1, 2010, Pages 6-10

  Ramada, Marcelo Henrique Soller ^a   Lopes, Fabyano Álvares Cardoso ^a   Ulhoa, Cirano José ^a   Silva, Roberto do Nascimento ^a  

^a FEDERAL UNIVERSITY OF GOIÁS   (Brazil)

Author keywords

1,3 glucanase; Biocontrol; Laminarin; Microplate assay; Trichoderma

Indexed keywords

1,3 BETA GLUCANASE;

ARTICLE; BACTERIUM ISOLATE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME MECHANISM; INTERMETHOD COMPARISON; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; RELIABILITY; REPRODUCIBILITY; SENSITIVITY ANALYSIS; TRICHODERMA;

ANIMALS; CLINICAL LABORATORY TECHNIQUES; GLUCAN 1,3-BETA-GLUCOSIDASE; MASS SCREENING; MYCOLOGY; SENSITIVITY AND SPECIFICITY; TRICHODERMA;

FUNGI; TRICHODERMA;

EID: 77649128623     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2010.01.010     Document Type: Article

References (25)

1. Adams D.J. Fungal cell wall chitinases and glucanases. Microbiology 150 (2004) 2029-2035
2. Almeida F.B., Cerqueira F.M., Silva R.N., Ulhoa C.J., and Lima A.L. Mycoparasitism studies of Trichoderma harzianum strains against Rhizoctonia solani: evaluation of coiling and hydrolytic enzyme production. Biotechnol. Lett. 29 (2007) 1189-1193
3. Bara M.T.F., Lima A.L., and Ulhoa C.J. Purification and characterization of an a exo-β-1, 3-glucanase produced by Trichoderma asperellum. FEMS Microbiol. Lett. 219 (2003) 81-85
4. Beffa R., and Meins Jr. F. Pathogenesis-related functions of plant β-1, 3-glucanase investigated by antisense transformation - a review. Gene 179 (1996) 97-103
5. Benitez T., Rincon A.M., Limon M.C., and Codon A.C. Biocontrol mechanisms of Trichoderma strains. Int. Microbiol. 7 (2004) 249-260
6. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976) 680-685
7. Buchner P., Rochat C., Wuillèm S., and Boutin J.P. Characterization of a tissue-specific and developmentally regulated β-1, 3-glucanase gene in pea (Pisum sativum). Plant Mol. Biol. 49 2002 (2002) 171-186
8. Cohen-Kupiec R., Broglie K.E., Friesam D., Broglie R.M., and Chet I. Molecular characterization of a novel β-1, 3-exoglucanase related to mycoparasitism of Trichoderma harzianum. Gene 226 (1999) 147-154
9. Cuadrado A.B.M., Fontaine T., Esteban P.F., Dedo J.E., Redondo M.M., Rey F., Latgé J.P., and Aldana C.R.V. Characterization of the endo-β-1, 3-glucanase activity of S. cerevisiae Eng2 and other members of the GH81 family. Fungal Genet. Biol. 45 (2008) 542-553
10. Genta F.A., Dumont A.F., Marana S.R., Terra W.R., and Ferreira C. The interplay of processivity, substrate inhibition and a secondary substrate binding site of an insect exo-β-1, 3-glucanase. Biochim. Biophys. Acta 1774 (2007) 1079-1091
11. Gueguen Y., Voorhorst W.G.B., Oost J., and Vos W.M. Molecular and Biochemical Characterization of an Endo-β-1, 3-glucanase of the Hyperthermophilic Archaeon Pyrococcus furiosus. J. Biol. Chem. 272 (1997) 31258-31264
12. Howell C.R. Mechanisms employed by Trichoderma species in the biological control of plant diseases: the history and evolution of current concepts. Plant Dis. 87 (2003) 4-10
13. Kubicek C.P., Mach R.L., Peterbauer C.K., and Lorito M. Trichoderma: from genes to biocontrol. J. Plant Pathol. 83 (2001) 11-23
14. Marcello C.M., Steindorff A.S., Silva S.P., Silva R.N., Bataus L.A.M., and Ulhoa C.J. Expression analysis of the exo-β-1, 3-glucanase from the mycoparasitic fungus Trichoderma asperellum. Microbiol. Res. 165 (2010) 75-81
15. Martin K., McDougall B.M., McIlroy S., Jayus, Chen J., and Seviour R.J. Biochemistry and molecular biology of exocellular fungal β-(1,3)- and β-(1,6)-glucanases. FEMS Microbiol. Rev. 31 (2007) 168-192
16. Miller G.L. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31 (1959) 426-428
17. Monteiro V.N., and Ulhoa C.J. Biochemical characterization of a β-1, 3-glucanase from Trichoderma koningii induced by cell wall of Rhizoctonia solani. Curr. Microbiol. 52 (2006) 92-96
18. Noronha E.F., Kipnis A., Junqueira-Kipnis A.P., and Ulhoa C.J. Regulation of a 36-kDa β-1, 3-glucanase synthesis in Trichoderma harzianum. FEMS Microbiol. Lett. 188 (2000) 19-22
19. Pitson S.M., Seviour R.J., and McDougall B.M. Noncellulolytic fungal β-glucanases: their physiology and regulation. Enzyme Microb. Technol. 15 (1993) 178-192
20. Prapagdee B., Kuekulvong C., and Mongkolsuk S. Antifungal potential of extracellular metabolites produced by Streptomyces hygroscopicus against phytopathogenic fungi. Int. J. Biol. Sci. 4 (2008) 330-337
21. Ramot O., Cohen-Kupiec R., and Chet I. Regulation of β-1, 3-glucanase by carbon starvation in the mycoparasite Trichoderma harzianum. Mycol. Res. 104 (2000) 415-420
22. Silva R.N., Monteiro V.N., Alcanfor J.D.X., Assis E.M., and Asquieri E.R. Comparison methods for the determination of reducers sugar and total in honey. Ciênc. Tecnol. Aliment. 23 (2003) 337-341
23. Watanabe T., Kasahara N., Aida K., and Tanaka H. Three N-terminal domains of β-1, 3-glucanase A1 are involved in binding to insoluble β-1, 3-glucan. J. Bacteriol. 174 (1992) 186-190
24. Xu Z., Shih M.C., and Poulton J.E. An extracellular exo-β-(1, 3)-glucanase from Pichia pastoris: purification, characterization, molecular cloning, and functional expression. Protein Expr. Purif. 47 (2006) 118-127
25. Zheng Y., and Wozniak C.A. Adaptation of a beta-1, 3-glucanase assay to microplate format. Biotechniques 22 (1997) 922-926