An improved purification approach with high cell viability and low cell loss for cryopreserved hepatocytes

Cryobiology

Volumn 60, Issue 2, 2010, Pages 238-239

  Meng, Fan ying ^a,b,c   Chen, Zhi shui ^a,b,c   Han, Meng ^a,b,c   Hu, Xin peng ^a,b,c   Zhou, Ping ^a,b,c  

^a HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b BEIJING UNIVERSITY OF POSTS AND TELECOMMUNICATIONS   (China)

^c BEIJING HOSPITAL   (China)

Author keywords

Cryopreservation; Density gradient centrifugation; Porcine hepatocytes

Indexed keywords

LIQUID NITROGEN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL COUNT; CELL CULTURE; CELL DEATH; CELL ISOLATION; CELL LOSS; CELL SIZE; CELL SUSPENSION; CELL VIABILITY; CRYOPRESERVATION; CULTURE MEDIUM; DENSITY GRADIENT CENTRIFUGATION; FREEZING; INCUBATION TEMPERATURE; LIVER CELL; NONHUMAN; PRIORITY JOURNAL; PURIFICATION; THAWING;

ANIMALS; CELL COUNT; CELL DEATH; CELL SEPARATION; CELL SURVIVAL; CENTRIFUGATION, DENSITY GRADIENT; CRYOPRESERVATION; FICOLL; HEPATOCYTES; SWINE;

SUIDAE; SUS;

EID: 77349110423     PISSN: 00112240     EISSN: 10902392     Source Type: Journal    
DOI: 10.1016/j.cryobiol.2009.11.003     Document Type: Article

References (9)

1. Adams R.M., Wang M., Crane A.M., Brown B., Darlington G.J., and Ledley F.D. Effective cryopreservation and long-term storage of primary human hepatocytes with recovery of viability, differentiation, and replicative potential. Cell Transplant. 4 (1995) 579-586
2. Alexandre E., Viollon-Abadie C., David P., Gandillet A., Coassolo P., Heyd B., Mantion G., Wolf P., Bachellier P., Jaeck D., and Richert L. Cryopreservation of adult human hepatocytes obtained from resected liver biopsies. Cryobiology 44 (2002) 103-113
3. Guillouzo A., Rialland L., Fautrel A., and Guyomard C. Survival and function of isolated hepatocytes after cryopreservation. Chem. Biol. Interact. 121 (1999) 7-16
4. Kobayashi N., Fujiwara T., Westerman K.A., Inoue Y., Sakaguchi M., Noguchi H., Miyazaki M., Cai J., Tanaka N., Fox I.J., and Leboulch P. Prevention of acute liver failure in rats with reversibly immortalized human hepatocytes. Science 287 (2000) 1258-1262
5. Koebe H.G., Dunn J.C., Toner M., Sterling L.M., Hubel A., Cravalho E.G., Yarmush M.L., and Tompkins R.G. A new approach to the cryopreservation of hepatocytes in a sandwich culture configuration. Cryobiology 27 (1990) 576-584
6. Koebe H.G., Dahnhardt C., Muller-Hocker J., Wagner H., and Schildberg F.W. Cryopreservation of porcine hepatocyte cultures. Cryobiology 33 (1996) 127-141
7. Maruyama M., Totsugawa T., Kunieda T., Okitsu T., Shibata N., Takesue M., Kurabayashi Y., Oshita M., Nakaji S., Kodama M., Tanaka N., and Kobayashi N. Hepatocyte isolation and transplantation in the pig. Cell Transplant. 12 (2003) 593-598
8. Terry C., Dhawan A., Mitry R.R., and Hughes R.D. Cryopreservation of isolated human hepatocytes for transplantation: state of the art. Cryobiology 53 (2006) 149-159
9. Terry C., Dhawan A., Mitry R.R., Lehec S.C., and Hughes R.D. Preincubation of rat and human hepatocytes with cytoprotectants prior to cryopreservation can improve viability and function upon thawing. Liver Transpl. 12 (2006) 165-177