Neonatal rat primary microglia: Isolation, culturing, and selected applications

Current Protocols in Toxicology

Volumn , Issue SUPPL. 43, 2010, Pages

  Ni, Mingwei ^a   Aschner, Michael ^a  

^a VANDERBILT UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Culture; In vitro; Isolation; Microglia

Indexed keywords

CELL ACTIVATION; CELL ISOLATION; CULTURE MEDIUM; IN VITRO STUDY; MICROGLIA; NERVE CELL CULTURE; NEUROMODULATION; NEUROTOXICOLOGY; NONHUMAN; PRIORITY JOURNAL; REPRODUCIBILITY; REVIEW;

ANIMALS; ANIMALS, NEWBORN; ASTROCYTES; BIOLOGICAL MARKERS; BRAIN; CELL CULTURE TECHNIQUES; IMMUNOHISTOCHEMISTRY; MICROGLIA; RATS;

RATTUS; RODENTIA;

EID: 77249179222     PISSN: 19349254     EISSN: 19349262     Source Type: Journal    
DOI: 10.1002/0471140856.tx1217s43     Document Type: Review

References (28)

1. Allen, N.J. and Barres, B.A. 2005. Signaling between glia and neurons: Focus on synaptic plasticity. Curr. Opin. Neurobiol. 15:542-548.
2. Aschner, M. and Allen, J.W. 2000. Astrocytes in methylmercury, ammonia, methionine sulfoximine and alcohol-induced neurotoxicity. Neurotoxicology 21:573-579.
3. Barger, S.W. and Basile, A.S. 2001. Activation of microglia by secreted amyloid precursor protein evokes release of glutamate by cystine exchange and attenuates synaptic function. J. Neurochem. 76:846-854.
4. Castellano, B., Gonzalez, B., Jensen, M.B., Pedersen, E.B., Finsen, B.R., and Zimmer, J. 1991. A double staining technique for simultaneous demonstration of astrocytes and microglia in brain sections and astroglial cell cultures. J. Histochem. Cytochem. 39:561-568.
5. Chang, J.Y. 2007. Methylmercury causes glial IL-6 release. Neurosci. Lett. 416:217-220.
6. Cole, R. and de Vellis, J. 1992. Astrocyte and oligodendrocyte cultures. In Protocols for NeuralCell Culture. (S. Federoff and A. Richardson, eds.) pp. 65-80. Humana, Totowa, N.J.
7. Dheen, S.T., Kaur, C., and Ling, E.A. 2007. Microglial activation and its implications in the brain diseases. Curr. Med. Chem. 14:1189-1197.
8. Frank, M.G., Wieseler-Frank, J.L., Watkins, L.R., and Maier, S.F. 2006. Rapid isolation of highly enriched and quiescent microglia from adult rat hippocampus: Immunophenotypic and functional characteristics. J. Neurosci. Methods 151:121-130.
9. Garg, T.K. and Chang, J.Y. 2006. Methylmercury causes oxidative stress and cytotoxicity in microglia: Attenuation by 15-deoxy-delta 12, 14-prostaglandin J2. J. Neuroimmunol. 171:17-28.
10. Hamilton, J.A., Hillard, C.J., Spector, A.A., and Watkins, P.A. 2007. Brain uptake and utilization of fatty acids, lipids and lipoproteins: Application to neurological disorders. J. Mol. Neurosci. 33:2-11.
11. Hassan, N.F., Campbell, D.E., Rifat, S., and Douglas, S.D. 1991a. Isolation and characterization of human fetal brain-derived microglia in in vitro culture. Neuroscience 41:149-158.
12. Hassan, N.F., Prakash, K., Chehimi, J., McCawley, L.J., and Douglas, S.D. 1991b. Isolation and characterization of newborn rabbit brain-derived microglia. Clin. Immunol. Immunopathol. 59:426-435.
13. Hassan, N.F., Rifat, S., Campbell, D.E., McCawley, L.J., and Douglas, S.D. 1991c. Isolation and flow cytometric characterization of newborn mouse brain-derived microglia maintained in vitro. J. Leukoc. Biol. 50:86-92.
14. Long, T.C., Tajuba, J., Sama, P., Saleh, N., Swartz, C., Parker, J., Hester, S., Lowry, G.V., and Veronisi, B. 2007. Nanosize titanium dioxide stimulates reactive oxygen species in brain microglia and damages neurons in vitro. Environ. Health Perspect. 115:1631-1637.
15. McCarthy, K.D. and de Vellis, J. 1980. Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue. J. Cell Biol. 85:890-902.
16. Migeon, B.R., Axelman, J., and Stetten, G. 1988. Clonal evolution in human lymphoblast cultures. Am. J. Hum. Genet. 42: 742-747.
17. Park, L.C., Zhang, H., and Gibson, G.E. 2001. Co-culture with astrocytes or microglia protects metabolically impaired neurons. Mech. Ageing Dev. 123:21-27.
18. Pocock, J.M. and Kettenmann, H. 2007. Neurotransmitter receptors on microglia. Trends Neurosci. 30:527-535.
19. Ransohoff, R.M. and Perry, V.H. 2009. Microglial physiology: Unique stimuli, specialized responses. Annu. Rev. Immunol. 27:119-145.
20. Rogers, J., Mastroeni, D., Leonard, B., Joyce, J., and Grover, A. 2007. Neuroinflammation in Alzheimer's disease and Parkinson's disease: Are microglia pathogenic in either disorder? Int. Rev. Neurobiol. 82:235-246.
21. Schwabe, T., Bainton, R.J., Fetter, R.D., Heberlein, U., and Gaul, U. 2005. GPCR signaling is required for blood-brain barrier formation in drosophila. Cell 123:133-144.
22. Shie, F.S., Nivison, M., Hsu, P.C., and Montine, T.J. 2009. Modulation of microglial innate immunity in Alzheimer's disease by activation of peroxisome proliferator-activated receptor gamma. Curr. Med. Chem. 16:643-651.
23. Vernadakis, A. 1988. Neuron-glia interrelations. Int. Rev. Neurobiol. 30:149-224.
24. Wang, Y., Cui, X., Tai, G., Ge, J., Li, N., Chen, F., You, F., and Lliu, Z. 2009. A critical role of activin A in maturation of mouse peritoneal macrophages in vitro and in vivo. Cell Mol. Immunol. 6:387-392.
25. Wilson, P.D. 1986. Use of cultured renal tubular cells in the study of cell injury. Miner Electrolyte Metab. 12:71-83.
26. Yang, I., Han, S.J., Kaur, G., Crane, C., and Parsa, A.T. 2009. The role of microglia in central nervous system immunity and glioma immunology. J. Clin. Neurosci. (In press).
27. Ye, S.M. and Johnson, R.W. 1999. Increased interleukin-6 expression by microglia from brain of aged mice. J. Neuroimmunol. 93:139-148.
28. Yin, Z., Aschner, J.L., dos Santos, A.P., and Aschner, M. 2008. Mitochondrial-dependent manganese neurotoxicity in rat primary astrocyte cultures. Brain Res. 1203:1-11.