Heterologous expression, purification, and biochemical characterization of a greenbug (Schizaphis graminum) acetylcholinesterase encoded by a paralogous gene (ace-1)

Journal of Biochemical and Molecular Toxicology

Volumn 24, Issue 1, 2010, Pages 51-59

  Zhao, Picheng ^a   Zhu, Kun Yan ^b   Jiang, Haobo ^a  

^a OKLAHOMA STATE UNIVERSITY   (United States)

^b KANSAS STATE UNIVERSITY   (United States)

Author keywords

Carbamate; Cholinergic synapse; Greenbug; Insecticide resistance; Organophosphate

Indexed keywords

1,5 BIS(4 ALLYLDIMETHYLAMMONIUMPHENYL)PENTAN 3 ONE DIBROMIDE; ACETYL(BETA METHYL)THIOCHOLINE IODIDE; ACETYLCHOLINESTERASE; ACETYLTHIOCHOLINE IODIDE; CARBARIL; DIMER; IODINE DERIVATIVE; MALAOXON; PARAOXON; PHYSOSTIGMINE; POLYACRYLAMIDE GEL; PROFENAMINE; PROPIONYLTHIOCHOLINE IODIDE; S BUTYRYLTHIOCHOLINE IODIDE; UNCLASSIFIED DRUG;

ARTICLE; BACULOVIRUS; CHOLINERGIC TRANSMISSION; DIMERIZATION; DISULFIDE BOND; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; HETEROLOGOUS EXPRESSION; IC 50; INSECT; MICHAELIS CONSTANT; NONHUMAN; PROTEIN SECRETION; REGULATORY MECHANISM; SCHIZAPHIS GRAMINUM; STRUCTURE ANALYSIS; VIRUS RECOMBINANT;

ACETYLCHOLINESTERASE; ACETYLTHIOCHOLINE; ANIMALS; APHIDS; CATALYTIC DOMAIN; CHOLINE; CHOLINESTERASE INHIBITORS; DIMERIZATION; ENZYME STABILITY; GENES, INSECT; HYDROGEN-ION CONCENTRATION; INSECT PROTEINS; KINETICS; MOLECULAR WEIGHT; RECOMBINANT FUSION PROTEINS; SUBSTRATE SPECIFICITY; TEMPERATURE; TRANSFECTION;

HEXAPODA; SCHIZAPHIS GRAMINUM;

EID: 77249141703     PISSN: 10956670     EISSN: 10990461     Source Type: Journal    
DOI: 10.1002/jbt.20311     Document Type: Article

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